ENSG00000178974

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000313833 ENSG00000178974 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO34 protein_coding protein_coding 11.98373 5.716925 17.0209 0.3389863 0.5963811 1.572322 9.0151104 4.6503686 11.9048776 0.4157298 0.6165739 1.3542482 0.76118333 0.8152667 0.70356667 -0.11170000 5.155997e-01 9.53812e-07 FALSE TRUE
ENST00000554940 ENSG00000178974 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO34 protein_coding processed_transcript 11.98373 5.716925 17.0209 0.3389863 0.5963811 1.572322 0.8187217 0.0000000 2.2436365 0.0000000 0.8285759 7.8161111 0.04433333 0.0000000 0.12900000 0.12900000 9.538120e-07 9.53812e-07 FALSE TRUE
MSTRG.9587.7 ENSG00000178974 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO34 protein_coding   11.98373 5.716925 17.0209 0.3389863 0.5963811 1.572322 0.3939105 0.5577199 0.2859733 0.3820246 0.2859733 -0.9397124 0.05498333 0.0956000 0.01573333 -0.07986667 4.694264e-01 9.53812e-07 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000178974 E001 0.0000000       14 55271344 55271369 26 +      
ENSG00000178974 E002 0.0000000       14 55271370 55271370 1 +      
ENSG00000178974 E003 0.1515154 0.0431366063 1.000000e+00   14 55271371 55271380 10 + 0.081 0.000 -9.728
ENSG00000178974 E004 3.1160928 0.0050746833 3.225460e-04 1.471072e-03 14 55271381 55271420 40 + 0.724 0.000 -15.661
ENSG00000178974 E005 3.5192424 0.0047093572 1.844288e-02 4.810479e-02 14 55271421 55271421 1 + 0.724 0.309 -2.051
ENSG00000178974 E006 3.9944126 0.0041258353 1.111157e-01 2.071802e-01 14 55271422 55271422 1 + 0.740 0.487 -1.118
ENSG00000178974 E007 19.7185517 0.0096402198 2.614391e-01 3.990851e-01 14 55271423 55271537 115 + 1.318 1.219 -0.350
ENSG00000178974 E008 3.0907047 0.3688291774 5.703479e-01 6.968451e-01 14 55271827 55271984 158 + 0.608 0.510 -0.452
ENSG00000178974 E009 0.6245948 0.1821575649 1.831819e-02 4.782774e-02 14 55272154 55272210 57 + 0.000 0.414 13.153
ENSG00000178974 E010 0.1515154 0.0431366063 1.000000e+00   14 55272211 55272281 71 + 0.081 0.000 -11.068
ENSG00000178974 E011 0.0000000       14 55272282 55272305 24 +      
ENSG00000178974 E012 0.2965864 0.0701847794 5.576048e-02   14 55278713 55278829 117 + 0.000 0.307 12.814
ENSG00000178974 E013 0.2934659 0.0283710183 5.090064e-01   14 55298041 55298105 65 + 0.081 0.181 1.339
ENSG00000178974 E014 0.0000000       14 55318183 55318269 87 +      
ENSG00000178974 E015 2.5401288 0.0060857315 4.627045e-01 6.025597e-01 14 55332817 55332945 129 + 0.484 0.614 0.604
ENSG00000178974 E016 16.4025653 0.0015044983 8.672071e-02 1.701559e-01 14 55350381 55350402 22 + 1.259 1.111 -0.528
ENSG00000178974 E017 58.8029982 0.0003958240 5.756182e-04 2.442966e-03 14 55350403 55350550 148 + 1.796 1.633 -0.553
ENSG00000178974 E018 102.8343557 0.0003988371 6.062501e-05 3.345522e-04 14 55350551 55350788 238 + 2.032 1.893 -0.469
ENSG00000178974 E019 598.1522430 0.0002079382 6.959418e-06 4.795900e-05 14 55350789 55352870 2082 + 2.762 2.726 -0.121
ENSG00000178974 E020 182.0579218 0.0011615261 5.698637e-17 2.524765e-15 14 55352871 55353611 741 + 2.146 2.388 0.811
ENSG00000178974 E021 0.1817044 0.0400526450 1.915577e-01   14 55354366 55354500 135 + 0.000 0.181 12.009
ENSG00000178974 E022 3.7086153 0.2955334226 5.916741e-02 1.252062e-01 14 55354501 55354585 85 + 0.387 0.943 2.435
ENSG00000178974 E023 6.1863109 0.2257252440 9.874731e-03 2.845705e-02 14 55355064 55355222 159 + 0.514 1.149 2.532
ENSG00000178974 E024 1.0351338 0.0411118393 2.863944e-01 4.267786e-01 14 55361733 55361918 186 + 0.208 0.408 1.346
ENSG00000178974 E025 4.9684700 0.0036926028 2.455428e-01 3.806906e-01 14 55367147 55368128 982 + 0.689 0.858 0.678
ENSG00000178974 E026 6.2937043 0.1011940199 2.426734e-04 1.144508e-03 14 55369861 55370053 193 + 0.484 1.175 2.768