ENSG00000178694

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000314622 ENSG00000178694 HEK293_OSMI2_2hA HEK293_TMG_2hB NSUN3 protein_coding protein_coding 5.593024 1.944538 8.468042 0.07212114 0.3240407 2.116903 1.9836205 0.8454359 3.1191029 0.03821178 0.07122163 1.8710174 0.37466667 0.4353000 0.36896667 -0.06633333 4.649020e-01 4.255112e-15 FALSE TRUE
ENST00000461625 ENSG00000178694 HEK293_OSMI2_2hA HEK293_TMG_2hB NSUN3 protein_coding processed_transcript 5.593024 1.944538 8.468042 0.07212114 0.3240407 2.116903 0.4493439 0.3356965 0.9472787 0.18109109 0.27345880 1.4694329 0.08700833 0.1709333 0.11330000 -0.05763333 9.862882e-01 4.255112e-15   FALSE
ENST00000468555 ENSG00000178694 HEK293_OSMI2_2hA HEK293_TMG_2hB NSUN3 protein_coding processed_transcript 5.593024 1.944538 8.468042 0.07212114 0.3240407 2.116903 0.4453194 0.0000000 0.6487110 0.00000000 0.21018482 6.0415738 0.05990417 0.0000000 0.07836667 0.07836667 1.359297e-02 4.255112e-15   FALSE
ENST00000477077 ENSG00000178694 HEK293_OSMI2_2hA HEK293_TMG_2hB NSUN3 protein_coding processed_transcript 5.593024 1.944538 8.468042 0.07212114 0.3240407 2.116903 0.4920192 0.6395992 0.3762572 0.11981416 0.13189741 -0.7499882 0.14731667 0.3306333 0.04353333 -0.28710000 2.727895e-04 4.255112e-15   FALSE
ENST00000483378 ENSG00000178694 HEK293_OSMI2_2hA HEK293_TMG_2hB NSUN3 protein_coding nonsense_mediated_decay 5.593024 1.944538 8.468042 0.07212114 0.3240407 2.116903 1.4967934 0.0000000 2.8670482 0.00000000 0.30422000 8.1684456 0.19054583 0.0000000 0.33683333 0.33683333 4.255112e-15 4.255112e-15   FALSE
MSTRG.23320.9 ENSG00000178694 HEK293_OSMI2_2hA HEK293_TMG_2hB NSUN3 protein_coding   5.593024 1.944538 8.468042 0.07212114 0.3240407 2.116903 0.2983810 0.0000000 0.0000000 0.00000000 0.00000000 0.0000000 0.05009583 0.0000000 0.00000000 0.00000000   4.255112e-15 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000178694 E001 1.5855339 0.0088109866 3.162900e-01 4.590425e-01 3 94062980 94062989 10 + 0.334 0.504 0.921
ENSG00000178694 E002 1.6962512 0.0085774172 8.875080e-01 9.320783e-01 3 94062990 94062997 8 + 0.406 0.422 0.091
ENSG00000178694 E003 3.9031497 0.0082990510 4.640194e-01 6.037731e-01 3 94062998 94063010 13 + 0.704 0.573 -0.566
ENSG00000178694 E004 13.4271353 0.0018285374 2.877968e-02 6.955877e-02 3 94063011 94063060 50 + 1.190 0.938 -0.916
ENSG00000178694 E005 26.7505459 0.0007866169 1.709490e-01 2.894119e-01 3 94063061 94063138 78 + 1.445 1.328 -0.404
ENSG00000178694 E006 9.9935334 0.0017942665 5.179292e-02 1.123500e-01 3 94063812 94063975 164 + 0.941 1.134 0.708
ENSG00000178694 E007 1.8499422 0.0711396947 4.388352e-01 5.806683e-01 3 94063976 94063979 4 + 0.371 0.507 0.714
ENSG00000178694 E008 4.9132107 0.0033769781 1.239844e-03 4.775924e-03 3 94063980 94064124 145 + 0.569 0.990 1.699
ENSG00000178694 E009 3.8489426 0.0071025254 1.388167e-05 8.922879e-05 3 94064125 94064403 279 + 0.371 0.990 2.697
ENSG00000178694 E010 33.4257607 0.0007490120 1.623613e-02 4.324498e-02 3 94064437 94064546 110 + 1.551 1.371 -0.621
ENSG00000178694 E011 0.2955422 0.0281941989 4.287670e-01   3 94076342 94076598 257 + 0.077 0.190 1.504
ENSG00000178694 E012 1.2995567 0.1398965746 9.408642e-01 9.668358e-01 3 94081609 94082173 565 + 0.335 0.324 -0.066
ENSG00000178694 E013 43.9800813 0.0007248003 3.315993e-01 4.752396e-01 3 94084107 94084354 248 + 1.612 1.652 0.133
ENSG00000178694 E014 24.9351902 0.0014067462 4.879239e-01 6.255589e-01 3 94084355 94084450 96 + 1.373 1.409 0.126
ENSG00000178694 E015 0.3666179 0.0318274425 4.227265e-01 5.658646e-01 3 94084451 94085382 932 + 0.077 0.190 1.510
ENSG00000178694 E016 29.6972156 0.0007016086 2.303991e-01 3.629383e-01 3 94094140 94094294 155 + 1.435 1.501 0.225
ENSG00000178694 E017 33.8400006 0.0006080771 7.709539e-01 8.521389e-01 3 94095033 94095154 122 + 1.519 1.486 -0.115
ENSG00000178694 E018 50.4055328 0.0022459028 2.230644e-01 3.540397e-01 3 94126211 94126444 234 + 1.663 1.715 0.178
ENSG00000178694 E019 230.5638864 0.0114224555 3.772825e-01 5.216217e-01 3 94126445 94131618 5174 + 2.344 2.283 -0.203
ENSG00000178694 E020 8.7690198 0.0249704405 2.716627e-01 4.104322e-01 3 94131619 94131832 214 + 0.921 1.054 0.494