ENSG00000178385

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000343987 ENSG00000178385 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHM3 protein_coding processed_transcript 2.825411 2.164325 3.008767 0.1717558 0.2983346 0.4733917 0.8286746 1.1635503 0.4740858 0.1301946 0.2546997 -1.277545 0.308325 0.5380333 0.1435333 -0.3945000 1.451672e-01 2.837294e-06   FALSE
ENST00000427836 ENSG00000178385 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHM3 protein_coding protein_coding 2.825411 2.164325 3.008767 0.1717558 0.2983346 0.4733917 1.7713925 0.9462601 2.0241556 0.1117789 0.0860938 1.088955 0.618875 0.4369000 0.6916667 0.2547667 1.720585e-01 2.837294e-06 FALSE TRUE
MSTRG.19860.4 ENSG00000178385 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHM3 protein_coding   2.825411 2.164325 3.008767 0.1717558 0.2983346 0.4733917 0.1569277 0.0000000 0.4967512 0.0000000 0.1436336 5.663206 0.049525 0.0000000 0.1605333 0.1605333 2.837294e-06 2.837294e-06 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000178385 E001 0.7728880 0.0164226625 1.770217e-02 4.649157e-02 2 207821288 207821289 2 - 0.000 0.410 11.284
ENSG00000178385 E002 40.9629054 0.0032093439 6.134694e-30 1.029406e-27 2 207821290 207821866 577 - 1.120 1.885 2.637
ENSG00000178385 E003 31.5154324 0.0047609241 2.316857e-13 6.259579e-12 2 207821867 207822084 218 - 1.186 1.732 1.885
ENSG00000178385 E004 319.5328963 0.0019019519 2.492382e-06 1.899644e-05 2 207822085 207826943 4859 - 2.451 2.561 0.369
ENSG00000178385 E005 5.5345779 0.0038779024 1.399025e-01 2.478896e-01 2 207826944 207826997 54 - 0.890 0.724 -0.653
ENSG00000178385 E006 21.6131271 0.0053970864 5.402007e-02 1.162987e-01 2 207826998 207827483 486 - 1.409 1.287 -0.424
ENSG00000178385 E007 8.7015802 0.0019205748 1.354923e-01 2.418054e-01 2 207827484 207827516 33 - 1.042 0.906 -0.507
ENSG00000178385 E008 28.8319132 0.0007408751 3.735508e-03 1.239860e-02 2 207827517 207828222 706 - 1.538 1.382 -0.539
ENSG00000178385 E009 15.2715536 0.0023865651 5.308796e-09 6.893794e-08 2 207828223 207828496 274 - 1.380 0.837 -1.970
ENSG00000178385 E010 0.0000000       2 207851238 207851737 500 -      
ENSG00000178385 E011 0.1515154 0.0433786922 6.449980e-01   2 207852783 207852835 53 - 0.104 0.000 -11.268
ENSG00000178385 E012 12.1446413 0.0014183686 2.709464e-06 2.048688e-05 2 207861105 207861262 158 - 1.269 0.812 -1.682
ENSG00000178385 E013 2.4281758 0.0104879263 3.931407e-03 1.294977e-02 2 207900041 207901307 1267 - 0.258 0.755 2.528
ENSG00000178385 E014 6.7319144 0.0024742974 2.072908e-03 7.449750e-03 2 207908514 207908577 64 - 1.020 0.655 -1.427
ENSG00000178385 E015 0.1451727 0.0432998765 6.454650e-01   2 207929897 207929940 44 - 0.104 0.000 -11.268
ENSG00000178385 E016 10.5619594 0.0018602695 2.550075e-02 6.291481e-02 2 207930926 207931119 194 - 1.146 0.946 -0.731
ENSG00000178385 E017 12.2553056 0.0015427760 1.459358e-03 5.504976e-03 2 207946367 207946512 146 - 1.230 0.946 -1.029
ENSG00000178385 E018 43.2622257 0.0005446813 7.035074e-13 1.769482e-11 2 207976651 207977586 936 - 1.776 1.409 -1.252
ENSG00000178385 E019 43.6973325 0.0005050660 1.778058e-13 4.883975e-12 2 208001030 208001957 928 - 1.784 1.409 -1.279
ENSG00000178385 E020 8.5729701 0.0045614641 2.225448e-02 5.620195e-02 2 208025389 208025540 152 - 1.073 0.837 -0.882