ENSG00000178295

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000317402 ENSG00000178295 HEK293_OSMI2_2hA HEK293_TMG_2hB GEN1 protein_coding protein_coding 5.509817 1.172432 8.18942 0.2220669 0.6766277 2.793765 0.72234355 0.23195410 1.0080942 0.23195410 0.5404457 2.0730658 0.113645833 0.1445333 0.12920000 -0.01533333 0.76288026 0.02089902 FALSE TRUE
ENST00000381254 ENSG00000178295 HEK293_OSMI2_2hA HEK293_TMG_2hB GEN1 protein_coding protein_coding 5.509817 1.172432 8.18942 0.2220669 0.6766277 2.793765 0.97203310 0.07471177 2.0501301 0.07471177 0.6061067 4.6040293 0.199100000 0.0716000 0.24473333 0.17313333 0.24515668 0.02089902 FALSE TRUE
ENST00000524465 ENSG00000178295 HEK293_OSMI2_2hA HEK293_TMG_2hB GEN1 protein_coding protein_coding 5.509817 1.172432 8.18942 0.2220669 0.6766277 2.793765 1.03820428 0.00000000 1.6041287 0.00000000 0.8028932 7.3346118 0.112787500 0.0000000 0.21353333 0.21353333 0.42576623 0.02089902 FALSE FALSE
ENST00000528873 ENSG00000178295 HEK293_OSMI2_2hA HEK293_TMG_2hB GEN1 protein_coding processed_transcript 5.509817 1.172432 8.18942 0.2220669 0.6766277 2.793765 0.10427594 0.33362796 0.0000000 0.18075371 0.0000000 -5.1027755 0.066345833 0.2747000 0.00000000 -0.27470000 0.02089902 0.02089902 FALSE TRUE
ENST00000534451 ENSG00000178295 HEK293_OSMI2_2hA HEK293_TMG_2hB GEN1 protein_coding protein_coding 5.509817 1.172432 8.18942 0.2220669 0.6766277 2.793765 0.71380850 0.00000000 0.6697309 0.00000000 0.6697309 6.0868918 0.071833333 0.0000000 0.07020000 0.07020000 1.00000000 0.02089902 FALSE FALSE
ENST00000534669 ENSG00000178295 HEK293_OSMI2_2hA HEK293_TMG_2hB GEN1 protein_coding retained_intron 5.509817 1.172432 8.18942 0.2220669 0.6766277 2.793765 0.06401159 0.00000000 0.5120927 0.00000000 0.5120927 5.7062342 0.006708333 0.0000000 0.05366667 0.05366667 1.00000000 0.02089902 FALSE TRUE
ENST00000614478 ENSG00000178295 HEK293_OSMI2_2hA HEK293_TMG_2hB GEN1 protein_coding processed_transcript 5.509817 1.172432 8.18942 0.2220669 0.6766277 2.793765 0.71231033 0.21174621 1.2165001 0.03890133 0.1190736 2.4675660 0.140350000 0.1827000 0.14820000 -0.03450000 0.73790467 0.02089902   FALSE
MSTRG.18001.12 ENSG00000178295 HEK293_OSMI2_2hA HEK293_TMG_2hB GEN1 protein_coding   5.509817 1.172432 8.18942 0.2220669 0.6766277 2.793765 0.13041705 0.07753981 0.1553796 0.07753981 0.1553796 0.9177701 0.027470833 0.0743000 0.02060000 -0.05370000 0.78038827 0.02089902 FALSE TRUE
MSTRG.18001.8 ENSG00000178295 HEK293_OSMI2_2hA HEK293_TMG_2hB GEN1 protein_coding   5.509817 1.172432 8.18942 0.2220669 0.6766277 2.793765 0.12264177 0.09193881 0.0000000 0.09193881 0.0000000 -3.3496315 0.081037500 0.0881000 0.00000000 -0.08810000 0.43290958 0.02089902 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000178295 E001 0.1451727 0.0427212985 1.000000e+00   2 17753857 17753857 1 + 0.059 0.000 -7.045
ENSG00000178295 E002 0.1451727 0.0427212985 1.000000e+00   2 17753858 17753865 8 + 0.059 0.000 -8.888
ENSG00000178295 E003 0.8386677 0.2614970012 2.594189e-01 3.968067e-01 2 17753866 17753884 19 + 0.157 0.395 1.767
ENSG00000178295 E004 0.9869609 0.1246334442 8.302718e-02 1.642896e-01 2 17753885 17753886 2 + 0.157 0.503 2.325
ENSG00000178295 E005 1.6544195 0.0091320969 8.957008e-02 1.744648e-01 2 17753887 17753894 8 + 0.273 0.592 1.735
ENSG00000178295 E006 1.8027127 0.0076823287 3.018886e-02 7.233985e-02 2 17753895 17753900 6 + 0.273 0.666 2.056
ENSG00000178295 E007 1.9542281 0.0074080623 4.705270e-02 1.039174e-01 2 17753901 17753903 3 + 0.305 0.666 1.833
ENSG00000178295 E008 2.7689358 0.0055516349 7.311157e-02 1.485018e-01 2 17753904 17753942 39 + 0.415 0.729 1.445
ENSG00000178295 E009 3.2065302 0.0051635277 1.579801e-01 2.724446e-01 2 17753943 17753967 25 + 0.483 0.729 1.097
ENSG00000178295 E010 2.2971161 0.0344483372 7.187582e-01 8.137510e-01 2 17753968 17754137 170 + 0.462 0.390 -0.382
ENSG00000178295 E011 1.8457921 0.0398148591 4.065490e-01 5.501746e-01 2 17754138 17754146 9 + 0.415 0.238 -1.137
ENSG00000178295 E012 2.7701981 0.0056397107 4.851707e-01 6.231004e-01 2 17754147 17754159 13 + 0.523 0.390 -0.681
ENSG00000178295 E013 15.9835803 0.0104329048 4.006284e-02 9.114669e-02 2 17754160 17754345 186 + 1.176 0.917 -0.944
ENSG00000178295 E014 27.1634524 0.0007748846 8.334928e-01 8.956935e-01 2 17754346 17755758 1413 + 1.345 1.372 0.093
ENSG00000178295 E015 3.6426452 0.0049404347 5.281430e-02 1.141612e-01 2 17759912 17759928 17 + 0.639 0.237 -2.205
ENSG00000178295 E016 8.8497077 0.0019305418 2.310343e-02 5.796264e-02 2 17759929 17759931 3 + 0.948 0.592 -1.436
ENSG00000178295 E017 23.2154718 0.0076072283 2.646133e-04 1.235464e-03 2 17759932 17760104 173 + 1.342 0.918 -1.526
ENSG00000178295 E018 16.4680404 0.0040407653 1.624926e-05 1.028778e-04 2 17761396 17761460 65 + 1.216 0.592 -2.408
ENSG00000178295 E019 25.1429869 0.0010068212 5.507407e-04 2.349491e-03 2 17761461 17761582 122 + 1.367 1.019 -1.238
ENSG00000178295 E020 32.6215211 0.0006133486 8.918850e-05 4.711794e-04 2 17764897 17765073 177 + 1.475 1.126 -1.223
ENSG00000178295 E021 0.3030308 0.2872576975 1.000000e+00   2 17765074 17765144 71 + 0.111 0.000 -9.323
ENSG00000178295 E022 29.5234494 0.0007082391 7.948087e-03 2.364877e-02 2 17766579 17766689 111 + 1.421 1.191 -0.803
ENSG00000178295 E023 25.3638263 0.0007793720 1.824813e-01 3.043342e-01 2 17768738 17768811 74 + 1.342 1.230 -0.392
ENSG00000178295 E024 3.6956482 0.0042783594 9.651421e-01 9.820621e-01 2 17770996 17771075 80 + 0.593 0.592 -0.004
ENSG00000178295 E025 3.5742080 0.0045073614 5.634167e-01 6.908762e-01 2 17771076 17771098 23 + 0.559 0.666 0.471
ENSG00000178295 E026 26.9727800 0.0027838989 3.776330e-02 8.689068e-02 2 17771196 17771287 92 + 1.378 1.192 -0.653
ENSG00000178295 E027 16.7613395 0.0015787708 6.707212e-02 1.384947e-01 2 17772634 17772647 14 + 1.188 0.988 -0.726
ENSG00000178295 E028 32.5968626 0.0006701041 9.336862e-03 2.712571e-02 2 17772648 17772784 137 + 1.460 1.248 -0.735
ENSG00000178295 E029 19.0652097 0.0012693171 3.337112e-01 4.774500e-01 2 17773096 17773132 37 + 1.216 1.126 -0.321
ENSG00000178295 E030 22.2339171 0.0024574219 4.394990e-01 5.812584e-01 2 17773219 17773299 81 + 1.277 1.212 -0.231
ENSG00000178295 E031 30.1447618 0.0076492355 1.383544e-01 2.458133e-01 2 17774271 17774401 131 + 1.417 1.283 -0.464
ENSG00000178295 E032 0.2987644 0.0271191590 1.000000e+00   2 17774402 17774472 71 + 0.111 0.000 -9.956
ENSG00000178295 E033 29.0136904 0.0007018084 1.057373e-01 1.992165e-01 2 17778002 17778063 62 + 1.396 1.266 -0.454
ENSG00000178295 E034 40.8688001 0.0005669601 7.588101e-03 2.274090e-02 2 17779978 17780121 144 + 1.551 1.358 -0.663
ENSG00000178295 E035 487.5378546 0.0005224128 9.728537e-24 9.374579e-22 2 17780621 17788946 8326 + 2.550 2.701 0.502