ENSG00000177963

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000526104 ENSG00000177963 HEK293_OSMI2_2hA HEK293_TMG_2hB RIC8A protein_coding protein_coding 84.47571 135.7504 54.68562 5.43007 0.9351447 -1.311565 17.816577 24.098543 15.449798 2.3932349 1.06232879 -0.6410230 0.22134583 0.17733333 0.28320000 0.10586667 0.020946457 4.530342e-10 FALSE TRUE
ENST00000526557 ENSG00000177963 HEK293_OSMI2_2hA HEK293_TMG_2hB RIC8A protein_coding retained_intron 84.47571 135.7504 54.68562 5.43007 0.9351447 -1.311565 4.634583 4.793948 2.744623 1.0174994 0.38665634 -0.8023658 0.05910417 0.03500000 0.05043333 0.01543333 0.514607921 4.530342e-10 FALSE TRUE
ENST00000532241 ENSG00000177963 HEK293_OSMI2_2hA HEK293_TMG_2hB RIC8A protein_coding retained_intron 84.47571 135.7504 54.68562 5.43007 0.9351447 -1.311565 5.332045 4.836355 3.641874 0.8912425 0.14607604 -0.4082629 0.07102917 0.03523333 0.06666667 0.03143333 0.006299874 4.530342e-10 FALSE FALSE
MSTRG.4877.1 ENSG00000177963 HEK293_OSMI2_2hA HEK293_TMG_2hB RIC8A protein_coding   84.47571 135.7504 54.68562 5.43007 0.9351447 -1.311565 4.327694 6.591490 2.403152 1.0403353 0.05417017 -1.4518728 0.05267083 0.04836667 0.04396667 -0.00440000 0.900977260 4.530342e-10 FALSE TRUE
MSTRG.4877.10 ENSG00000177963 HEK293_OSMI2_2hA HEK293_TMG_2hB RIC8A protein_coding   84.47571 135.7504 54.68562 5.43007 0.9351447 -1.311565 8.911663 15.548457 3.581778 0.5819218 2.51452815 -2.1149290 0.09891667 0.11513333 0.06646667 -0.04866667 0.654064927 4.530342e-10 FALSE TRUE
MSTRG.4877.8 ENSG00000177963 HEK293_OSMI2_2hA HEK293_TMG_2hB RIC8A protein_coding   84.47571 135.7504 54.68562 5.43007 0.9351447 -1.311565 19.397532 34.356871 9.622852 3.1296803 2.77775568 -1.8349836 0.22442083 0.25236667 0.17520000 -0.07716667 0.493524507 4.530342e-10 FALSE TRUE
MSTRG.4877.9 ENSG00000177963 HEK293_OSMI2_2hA HEK293_TMG_2hB RIC8A protein_coding   84.47571 135.7504 54.68562 5.43007 0.9351447 -1.311565 11.632800 25.860148 6.696681 2.7065748 0.46079132 -1.9476174 0.12175833 0.19033333 0.12226667 -0.06806667 0.030965200 4.530342e-10 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000177963 E001 49.7950303 6.541637e-03 2.552031e-01 3.920377e-01 11 207454 207707 254 + 1.558 1.643 0.291
ENSG00000177963 E002 557.8773219 3.006516e-03 2.202895e-05 1.352295e-04 11 207708 208529 822 + 2.763 2.639 -0.415
ENSG00000177963 E003 263.9830514 2.131169e-03 4.967057e-10 7.777453e-09 11 208530 208799 270 + 2.488 2.292 -0.654
ENSG00000177963 E004 80.7647246 1.018396e-02 3.200347e-02 7.586572e-02 11 208800 208802 3 + 1.934 1.800 -0.449
ENSG00000177963 E005 93.1594816 6.475005e-03 7.256434e-03 2.189237e-02 11 208803 208810 8 + 2.002 1.858 -0.485
ENSG00000177963 E006 100.2285456 1.829056e-03 5.829375e-05 3.230627e-04 11 208811 208812 2 + 2.051 1.880 -0.574
ENSG00000177963 E007 105.4319854 1.086715e-03 1.021086e-05 6.773165e-05 11 208813 208815 3 + 2.075 1.900 -0.588
ENSG00000177963 E008 197.4105236 2.109222e-04 1.812242e-03 6.637997e-03 11 208816 208836 21 + 2.284 2.197 -0.293
ENSG00000177963 E009 199.1011114 2.060392e-04 2.080483e-03 7.473857e-03 11 208837 208840 4 + 2.288 2.202 -0.287
ENSG00000177963 E010 240.1629386 6.421029e-04 9.663775e-05 5.062979e-04 11 208841 208854 14 + 2.383 2.277 -0.355
ENSG00000177963 E011 459.3512792 1.895800e-03 4.445187e-01 5.860724e-01 11 208855 208938 84 + 2.599 2.582 -0.057
ENSG00000177963 E012 0.3289534 2.959524e-02 4.582067e-01   11 209069 209079 11 + 0.188 0.078 -1.464
ENSG00000177963 E013 5.1475344 3.720694e-03 9.959685e-07 8.266154e-06 11 209080 209174 95 + 1.111 0.471 -2.602
ENSG00000177963 E014 15.9250221 4.173471e-02 9.505380e-03 2.755107e-02 11 209175 209258 84 + 1.385 1.058 -1.161
ENSG00000177963 E015 16.8605543 3.145768e-02 6.848785e-01 7.877180e-01 11 209259 209270 12 + 1.223 1.171 -0.184
ENSG00000177963 E016 434.5032027 3.873757e-03 1.740424e-01 2.934349e-01 11 209271 209318 48 + 2.597 2.553 -0.148
ENSG00000177963 E017 3.8837058 1.096943e-01 3.278381e-03 1.108674e-02 11 209319 209335 17 + 0.986 0.413 -2.451
ENSG00000177963 E018 7.2696697 3.419204e-02 2.043074e-02 5.239808e-02 11 209336 209406 71 + 1.073 0.744 -1.251
ENSG00000177963 E019 521.0358818 1.028219e-03 8.421236e-01 9.015828e-01 11 209407 209478 72 + 2.633 2.642 0.028
ENSG00000177963 E020 784.5028766 1.314558e-03 7.833836e-01 8.609605e-01 11 209479 209629 151 + 2.818 2.817 0.000
ENSG00000177963 E021 625.5191324 1.147237e-03 4.110601e-01 5.546509e-01 11 209630 209744 115 + 2.701 2.725 0.079
ENSG00000177963 E022 962.0116170 7.746262e-04 9.488325e-01 9.719294e-01 11 209745 210000 256 + 2.902 2.908 0.020
ENSG00000177963 E023 0.5106578 2.678517e-01 7.840815e-01 8.614982e-01 11 210364 210406 43 + 0.188 0.144 -0.456
ENSG00000177963 E024 2.6379808 4.656077e-02 9.080101e-01 9.456001e-01 11 210407 210445 39 + 0.500 0.523 0.112
ENSG00000177963 E025 1.9038000 8.500538e-02 1.910564e-01 3.150712e-01 11 210446 210570 125 + 0.188 0.470 1.854
ENSG00000177963 E026 317.4518509 1.841938e-04 7.481835e-01 8.354964e-01 11 210571 210574 4 + 2.429 2.424 -0.019
ENSG00000177963 E027 634.4833747 9.918714e-05 4.041996e-01 5.478992e-01 11 210575 210662 88 + 2.734 2.722 -0.039
ENSG00000177963 E028 10.5499942 4.421038e-03 6.747400e-05 3.679441e-04 11 210663 211198 536 + 1.275 0.861 -1.510
ENSG00000177963 E029 784.8231876 1.010155e-03 6.502885e-01 7.613659e-01 11 211199 211349 151 + 2.809 2.823 0.045
ENSG00000177963 E030 44.0363466 7.357573e-04 3.283317e-26 3.984718e-24 11 211350 212415 1066 + 1.937 1.372 -1.923
ENSG00000177963 E031 503.1824138 1.395251e-04 3.214644e-01 4.647152e-01 11 212416 212467 52 + 2.609 2.630 0.069
ENSG00000177963 E032 494.9622472 2.078693e-04 5.961693e-02 1.259731e-01 11 212468 212511 44 + 2.588 2.627 0.130
ENSG00000177963 E033 8.2734741 2.407869e-03 8.735352e-07 7.346561e-06 11 212512 212596 85 + 1.250 0.709 -2.028
ENSG00000177963 E034 12.1207874 1.502585e-03 3.797711e-03 1.257564e-02 11 212597 212614 18 + 1.250 0.965 -1.027
ENSG00000177963 E035 714.2402673 9.966279e-05 9.524450e-05 4.997171e-04 11 212615 212727 113 + 2.726 2.790 0.215
ENSG00000177963 E036 501.8531696 1.156104e-04 4.787499e-03 1.533922e-02 11 212728 212759 32 + 2.579 2.634 0.185
ENSG00000177963 E037 46.8373909 4.806298e-04 2.762333e-07 2.574992e-06 11 212760 212836 77 + 1.797 1.520 -0.940
ENSG00000177963 E038 549.9170030 1.334588e-04 1.119145e-01 2.083776e-01 11 212837 212869 33 + 2.638 2.669 0.103
ENSG00000177963 E039 757.7359750 2.821113e-04 1.822020e-03 6.670331e-03 11 212870 212981 112 + 2.758 2.814 0.185
ENSG00000177963 E040 29.8147607 1.161421e-02 1.041711e-07 1.054384e-06 11 212982 213035 54 + 1.701 1.271 -1.478
ENSG00000177963 E041 59.0008613 1.303983e-02 3.178753e-07 2.926025e-06 11 213036 213298 263 + 1.950 1.590 -1.216
ENSG00000177963 E042 835.8245173 2.058793e-04 8.287736e-06 5.610258e-05 11 213299 213418 120 + 2.787 2.860 0.242
ENSG00000177963 E043 13.3801565 4.684698e-02 4.827203e-02 1.061158e-01 11 213419 213448 30 + 1.263 1.003 -0.937
ENSG00000177963 E044 12.5611864 1.150568e-02 1.359952e-03 5.176484e-03 11 213449 213497 49 + 1.288 0.948 -1.223
ENSG00000177963 E045 16.0191801 1.115860e-03 1.388194e-08 1.666387e-07 11 213498 213780 283 + 1.472 0.992 -1.701
ENSG00000177963 E046 19.3822339 2.837393e-03 4.770854e-03 1.529210e-02 11 214206 214229 24 + 1.404 1.168 -0.827
ENSG00000177963 E047 546.3565727 1.389705e-04 4.283697e-05 2.452430e-04 11 214230 214287 58 + 2.598 2.676 0.260
ENSG00000177963 E048 2222.9473566 1.400564e-03 1.994728e-07 1.911168e-06 11 214288 215113 826 + 3.193 3.293 0.334