ENSG00000177885

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000316804 ENSG00000177885 HEK293_OSMI2_2hA HEK293_TMG_2hB GRB2 protein_coding protein_coding 77.69708 100.3568 70.26804 5.730113 3.098041 -0.5141361 60.990221 67.52807 62.2231 7.416541 1.491959 -0.1180187 0.7854958 0.6806333 0.8881 0.2074667 9.576339e-02 2.174506e-19 FALSE TRUE
ENST00000392564 ENSG00000177885 HEK293_OSMI2_2hA HEK293_TMG_2hB GRB2 protein_coding protein_coding 77.69708 100.3568 70.26804 5.730113 3.098041 -0.5141361 9.934295 29.74795 0.0000 10.031718 0.000000 -11.5390597 0.1208375 0.2895000 0.0000 -0.2895000 2.174506e-19 2.174506e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000177885 E001 1.5532541 0.0472971418 2.874290e-01 4.279223e-01 17 75289729 75289849 121 - 0.490 0.305 -1.038
ENSG00000177885 E002 57.6317994 0.0051972488 5.781179e-09 7.463725e-08 17 75318076 75318120 45 - 1.444 1.848 1.375
ENSG00000177885 E003 167.1232965 0.0005010147 3.096728e-20 2.029986e-18 17 75318121 75318178 58 - 1.965 2.290 1.089
ENSG00000177885 E004 492.9682231 0.0006354836 1.190766e-16 5.070513e-15 17 75318179 75318297 119 - 2.533 2.728 0.652
ENSG00000177885 E005 1699.3318196 0.0040947985 4.243429e-05 2.431360e-04 17 75318298 75318754 457 - 3.106 3.248 0.471
ENSG00000177885 E006 553.5461322 0.0018250308 1.215353e-04 6.208740e-04 17 75318755 75318796 42 - 2.637 2.758 0.401
ENSG00000177885 E007 2137.9558218 0.0014593712 1.806458e-18 9.640224e-17 17 75318797 75319478 682 - 3.180 3.357 0.589
ENSG00000177885 E008 891.0900130 0.0014134886 7.467787e-01 8.344441e-01 17 75319479 75319830 352 - 2.910 2.933 0.076
ENSG00000177885 E009 667.1394013 0.0008584452 4.001807e-04 1.775065e-03 17 75319831 75320010 180 - 2.834 2.782 -0.173
ENSG00000177885 E010 1042.6533203 0.0002353943 1.007706e-01 1.916352e-01 17 75320011 75320340 330 - 2.997 2.994 -0.009
ENSG00000177885 E011 259.5761615 0.0010127076 9.235697e-05 4.860749e-04 17 75320341 75320344 4 - 2.449 2.363 -0.289
ENSG00000177885 E012 978.3516152 0.0001111129 1.727535e-13 4.752778e-12 17 75320345 75320550 206 - 3.014 2.944 -0.230
ENSG00000177885 E013 427.3166043 0.0001427276 5.833567e-05 3.232478e-04 17 75320551 75320553 3 - 2.644 2.592 -0.175
ENSG00000177885 E014 862.2257945 0.0001283393 2.018796e-08 2.352266e-07 17 75321659 75321794 136 - 2.948 2.895 -0.177
ENSG00000177885 E015 534.4695798 0.0004985318 1.170650e-02 3.283382e-02 17 75321795 75321827 33 - 2.724 2.695 -0.096
ENSG00000177885 E016 828.4501802 0.0012076531 1.262542e-04 6.424000e-04 17 75325898 75326020 123 - 2.934 2.872 -0.206
ENSG00000177885 E017 0.5117019 0.0243967742 2.076796e-01 3.356962e-01 17 75326239 75326338 100 - 0.000 0.245 10.175
ENSG00000177885 E018 720.3226943 0.0014231234 1.944475e-08 2.273498e-07 17 75332700 75332797 98 - 2.902 2.795 -0.356
ENSG00000177885 E019 0.0000000       17 75387490 75387794 305 -      
ENSG00000177885 E020 1.5187246 0.0100265995 1.375793e-01 2.447129e-01 17 75393404 75393550 147 - 0.546 0.303 -1.314
ENSG00000177885 E021 780.4378234 0.0053169450 9.571487e-08 9.753064e-07 17 75393551 75393765 215 - 2.978 2.799 -0.596
ENSG00000177885 E022 6.2497501 0.0223356284 2.373057e-01 3.710567e-01 17 75393766 75393864 99 - 0.931 0.783 -0.572
ENSG00000177885 E023 0.4428904 0.5915137900 5.393204e-01 6.706170e-01 17 75393919 75394033 115 - 0.000 0.187 9.683
ENSG00000177885 E024 1.5050942 0.1093924071 3.615752e-01 5.059799e-01 17 75404841 75405488 648 - 0.490 0.306 -1.032
ENSG00000177885 E025 279.7058829 0.0153382869 5.773041e-06 4.051737e-05 17 75405489 75405709 221 - 2.584 2.311 -0.911