ENSG00000177853

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000316045 ENSG00000177853 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF518A protein_coding protein_coding 5.136344 1.196316 8.900449 0.2955296 0.1505785 2.884891 1.1996728 0.27197131 2.4806639 0.15107102 0.12247912 3.1429101 0.22009583 0.32383333 0.27903333 -0.04480000 9.293183e-01 9.810613e-06 FALSE TRUE
ENST00000484770 ENSG00000177853 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF518A protein_coding retained_intron 5.136344 1.196316 8.900449 0.2955296 0.1505785 2.884891 0.1445014 0.17674289 0.1352742 0.13301542 0.07822117 -0.3622752 0.04264583 0.11153333 0.01490000 -0.09663333 4.410599e-01 9.810613e-06   FALSE
ENST00000534948 ENSG00000177853 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF518A protein_coding retained_intron 5.136344 1.196316 8.900449 0.2955296 0.1505785 2.884891 0.7690560 0.02477642 0.6512648 0.02477642 0.10156590 4.2490468 0.17059583 0.01416667 0.07323333 0.05906667 1.324754e-01 9.810613e-06 FALSE TRUE
ENST00000614149 ENSG00000177853 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF518A protein_coding protein_coding 5.136344 1.196316 8.900449 0.2955296 0.1505785 2.884891 0.4963213 0.07349101 0.8682888 0.04398723 0.27916182 3.3950025 0.07555417 0.08133333 0.09793333 0.01660000 8.116855e-01 9.810613e-06 FALSE TRUE
ENST00000624776 ENSG00000177853 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF518A protein_coding protein_coding 5.136344 1.196316 8.900449 0.2955296 0.1505785 2.884891 0.7714749 0.47246378 1.3137088 0.29051628 0.27886048 1.4560933 0.23380000 0.31713333 0.14663333 -0.17050000 7.350625e-01 9.810613e-06 FALSE TRUE
MSTRG.4421.6 ENSG00000177853 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF518A protein_coding   5.136344 1.196316 8.900449 0.2955296 0.1505785 2.884891 0.7891112 0.00000000 1.3034741 0.00000000 0.07439008 7.0372440 0.09445833 0.00000000 0.14653333 0.14653333 9.810613e-06 9.810613e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000177853 E001 0.0000000       10 96129058 96129369 312 +      
ENSG00000177853 E002 0.0000000       10 96129370 96129652 283 +      
ENSG00000177853 E003 0.0000000       10 96129653 96129714 62 +      
ENSG00000177853 E004 1.0520752 0.2749764938 5.644521e-01 6.917473e-01 10 96129715 96129734 20 + 0.261 0.000 -9.736
ENSG00000177853 E005 8.6099493 0.0019706841 7.770354e-01 8.564722e-01 10 96129735 96129992 258 + 0.843 0.809 -0.133
ENSG00000177853 E006 1.1918355 0.1493533623 9.497664e-01 9.725705e-01 10 96129993 96130055 63 + 0.260 0.278 0.131
ENSG00000177853 E007 2.3615224 0.0291143050 4.765924e-01 6.152111e-01 10 96130056 96130071 16 + 0.440 0.280 -0.955
ENSG00000177853 E008 3.9751464 0.0545235496 1.729891e-01 2.920801e-01 10 96130072 96130118 47 + 0.606 0.279 -1.749
ENSG00000177853 E009 7.7804929 0.0021893337 6.311955e-01 7.462567e-01 10 96130297 96130678 382 + 0.813 0.743 -0.279
ENSG00000177853 E010 6.6639863 0.0195797387 6.055094e-01 7.258825e-01 10 96130679 96130752 74 + 0.754 0.666 -0.364
ENSG00000177853 E011 1.4717847 0.2951413949 4.599371e-01 6.000034e-01 10 96130753 96130822 70 + 0.259 0.459 1.204
ENSG00000177853 E012 17.8358013 0.0094372814 1.605853e-01 2.759093e-01 10 96132586 96132670 85 + 1.141 0.962 -0.656
ENSG00000177853 E013 1.2586568 0.0108647759 6.252781e-02 1.308976e-01 10 96132671 96132909 239 + 0.200 0.570 2.216
ENSG00000177853 E014 3.6496533 0.0176205433 6.045550e-01 7.250556e-01 10 96133556 96133582 27 + 0.553 0.449 -0.503
ENSG00000177853 E015 16.6771154 0.0010711739 2.135076e-01 3.426667e-01 10 96133583 96133648 66 + 1.111 0.962 -0.549
ENSG00000177853 E016 2.2980345 0.0067842498 5.174715e-01 6.518527e-01 10 96135170 96135183 14 + 0.421 0.280 -0.853
ENSG00000177853 E017 3.9255019 0.0040068759 5.009305e-01 6.371452e-01 10 96135184 96135289 106 + 0.580 0.449 -0.631
ENSG00000177853 E018 9.1700186 0.0018355818 2.400310e-03 8.462012e-03 10 96151416 96151566 151 + 0.910 0.280 -2.977
ENSG00000177853 E019 13.7523726 0.0012586887 5.836756e-05 3.233583e-04 10 96155326 96155342 17 + 1.070 0.280 -3.570
ENSG00000177853 E020 18.2112076 0.0027582862 3.178829e-03 1.079363e-02 10 96155343 96155376 34 + 1.167 0.743 -1.594
ENSG00000177853 E021 2.9549978 0.0053228345 8.698449e-01 9.202879e-01 10 96155377 96155398 22 + 0.475 0.449 -0.132
ENSG00000177853 E022 4.2020039 0.0043195848 1.244486e-01 2.263177e-01 10 96155399 96155923 525 + 0.619 0.280 -1.800
ENSG00000177853 E023 22.4848074 0.0008685012 1.827646e-04 8.897438e-04 10 96155924 96156012 89 + 1.257 0.743 -1.914
ENSG00000177853 E024 12.3113686 0.0072427098 2.604940e-04 1.218725e-03 10 96156013 96156047 35 + 1.025 0.280 -3.405
ENSG00000177853 E025 252.9528384 0.0004600389 4.867243e-22 3.898878e-20 10 96156197 96158869 2673 + 2.272 1.931 -1.141
ENSG00000177853 E026 21.1956790 0.0352432304 1.015214e-01 1.927899e-01 10 96158870 96158953 84 + 1.215 0.964 -0.908
ENSG00000177853 E027 169.3036453 0.0017312194 5.211028e-02 1.129186e-01 10 96158954 96160402 1449 + 2.072 2.005 -0.227
ENSG00000177853 E028 280.9388488 0.0008021709 3.443299e-50 2.124829e-47 10 96160403 96163761 3359 + 2.218 2.562 1.148
ENSG00000177853 E029 0.0000000       10 96197121 96197166 46 +      
ENSG00000177853 E030 0.1482932 0.0422645271 4.348222e-02   10 96203574 96203628 55 + 0.000 0.280 11.714
ENSG00000177853 E031 2.6508436 0.0058580455 1.932995e-04 9.351143e-04 10 96203920 96205288 1369 + 0.287 0.916 2.955