ENSG00000177666

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000336615 ENSG00000177666 HEK293_OSMI2_2hA HEK293_TMG_2hB PNPLA2 protein_coding protein_coding 85.72701 131.8268 55.1137 8.124518 1.996857 -1.258008 39.865307 71.397139 23.990566 4.2445922 0.8246997 -1.5729999 0.43744167 0.54176667 0.4371667 -0.10460000 4.155771e-02 1.259149e-09 FALSE TRUE
ENST00000525250 ENSG00000177666 HEK293_OSMI2_2hA HEK293_TMG_2hB PNPLA2 protein_coding retained_intron 85.72701 131.8268 55.1137 8.124518 1.996857 -1.258008 6.371132 4.076246 5.479066 0.6298760 0.6464961 0.4257846 0.08915833 0.03073333 0.0988000 0.06806667 1.414082e-08 1.259149e-09 FALSE TRUE
ENST00000526083 ENSG00000177666 HEK293_OSMI2_2hA HEK293_TMG_2hB PNPLA2 protein_coding retained_intron 85.72701 131.8268 55.1137 8.124518 1.996857 -1.258008 30.917290 51.027217 17.110487 2.3386759 1.7176490 -1.5758259 0.35480833 0.38786667 0.3097667 -0.07810000 1.213718e-01 1.259149e-09   FALSE
ENST00000531923 ENSG00000177666 HEK293_OSMI2_2hA HEK293_TMG_2hB PNPLA2 protein_coding retained_intron 85.72701 131.8268 55.1137 8.124518 1.996857 -1.258008 4.573663 2.821672 4.960281 0.6558645 0.4449119 0.8116734 0.06511250 0.02096667 0.0897000 0.06873333 1.259149e-09 1.259149e-09   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000177666 E001 1.460053 0.3532533347 9.584922e-01 9.779956e-01 11 818914 818958 45 + 0.324 0.340 0.100
ENSG00000177666 E002 1.249090 0.0136135747 8.832814e-01 9.293367e-01 11 819556 819573 18 + 0.325 0.291 -0.221
ENSG00000177666 E003 519.550217 0.0132334910 1.568487e-01 2.709101e-01 11 819574 819905 332 + 2.696 2.615 -0.270
ENSG00000177666 E004 180.603177 0.0093276828 1.078391e-23 1.033374e-21 11 820835 821627 793 + 2.531 1.982 -1.836
ENSG00000177666 E005 726.711142 0.0010981132 3.278492e-03 1.108692e-02 11 821628 821773 146 + 2.833 2.762 -0.236
ENSG00000177666 E006 812.648294 0.0001228443 6.815288e-05 3.712294e-04 11 821774 821860 87 + 2.878 2.809 -0.229
ENSG00000177666 E007 736.578227 0.0001597185 5.787771e-04 2.455383e-03 11 821958 822015 58 + 2.832 2.768 -0.214
ENSG00000177666 E008 505.380823 0.0003688946 1.337343e-02 3.678805e-02 11 822016 822023 8 + 2.665 2.606 -0.197
ENSG00000177666 E009 48.787992 0.0037294010 9.111360e-24 8.797756e-22 11 822024 822396 373 + 1.988 1.396 -2.009
ENSG00000177666 E010 1235.382184 0.0001174158 4.530212e-01 5.938192e-01 11 822397 822606 210 + 3.023 3.003 -0.066
ENSG00000177666 E011 428.669297 0.0004249878 1.053947e-02 3.006537e-02 11 823527 823542 16 + 2.514 2.560 0.154
ENSG00000177666 E012 500.373347 0.0001723116 1.418745e-06 1.140141e-05 11 823543 823587 45 + 2.549 2.633 0.279
ENSG00000177666 E013 10.268330 0.0209344458 5.407758e-04 2.312654e-03 11 823588 823647 60 + 1.261 0.830 -1.581
ENSG00000177666 E014 11.024315 0.0118569820 4.138640e-02 9.360038e-02 11 823648 823693 46 + 1.173 0.928 -0.895
ENSG00000177666 E015 895.607018 0.0001873577 1.884728e-08 2.208275e-07 11 823694 823855 162 + 2.809 2.883 0.246
ENSG00000177666 E016 24.050701 0.0008864605 1.927895e-09 2.708646e-08 11 823856 823997 142 + 1.619 1.179 -1.526
ENSG00000177666 E017 909.864008 0.0001418617 1.130781e-09 1.660258e-08 11 823998 824130 133 + 2.812 2.891 0.260
ENSG00000177666 E018 913.360730 0.0005890883 5.353157e-04 2.291893e-03 11 824314 824436 123 + 2.836 2.887 0.170
ENSG00000177666 E019 7.581493 0.0085061289 3.023383e-01 4.442078e-01 11 824437 824437 1 + 0.970 0.827 -0.545
ENSG00000177666 E020 484.158383 0.0009839843 2.331531e-03 8.248703e-03 11 824523 824556 34 + 2.548 2.614 0.218
ENSG00000177666 E021 1683.139408 0.0003270778 7.978652e-07 6.766682e-06 11 824557 826143 1587 + 3.102 3.153 0.171