ENSG00000177463

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000323373 ENSG00000177463 HEK293_OSMI2_2hA HEK293_TMG_2hB NR2C2 protein_coding protein_coding 8.732579 4.966933 11.07937 0.3921004 0.3984612 1.155849 0.6476962 0.4924437 0.2477736 0.20091708 0.13117695 -0.9628574 0.07187083 0.09933333 0.02156667 -0.07776667 2.894151e-01 1.354036e-07 FALSE TRUE
ENST00000393102 ENSG00000177463 HEK293_OSMI2_2hA HEK293_TMG_2hB NR2C2 protein_coding protein_coding 8.732579 4.966933 11.07937 0.3921004 0.3984612 1.155849 0.7293302 0.4110635 0.6200093 0.41106353 0.62000934 0.5813353 0.08909583 0.07200000 0.06030000 -0.01170000 9.282161e-01 1.354036e-07 FALSE TRUE
ENST00000406272 ENSG00000177463 HEK293_OSMI2_2hA HEK293_TMG_2hB NR2C2 protein_coding protein_coding 8.732579 4.966933 11.07937 0.3921004 0.3984612 1.155849 1.5962381 0.4588934 0.0000000 0.45889337 0.00000000 -5.5511880 0.18179583 0.10483333 0.00000000 -0.10483333 6.861595e-01 1.354036e-07 FALSE TRUE
ENST00000425241 ENSG00000177463 HEK293_OSMI2_2hA HEK293_TMG_2hB NR2C2 protein_coding protein_coding 8.732579 4.966933 11.07937 0.3921004 0.3984612 1.155849 1.8087737 1.3487638 3.9658308 0.28131406 0.13652478 1.5489617 0.21171667 0.26903333 0.35923333 0.09020000 4.143549e-01 1.354036e-07 FALSE TRUE
ENST00000435454 ENSG00000177463 HEK293_OSMI2_2hA HEK293_TMG_2hB NR2C2 protein_coding protein_coding 8.732579 4.966933 11.07937 0.3921004 0.3984612 1.155849 0.1902714 0.0000000 0.7065406 0.00000000 0.07410457 6.1629766 0.01987917 0.00000000 0.06363333 0.06363333 1.354036e-07 1.354036e-07   FALSE
ENST00000437120 ENSG00000177463 HEK293_OSMI2_2hA HEK293_TMG_2hB NR2C2 protein_coding protein_coding 8.732579 4.966933 11.07937 0.3921004 0.3984612 1.155849 0.2613060 0.3728756 0.0000000 0.37287564 0.00000000 -5.2588040 0.03128750 0.06530000 0.00000000 -0.06530000 6.651850e-01 1.354036e-07 FALSE FALSE
ENST00000475707 ENSG00000177463 HEK293_OSMI2_2hA HEK293_TMG_2hB NR2C2 protein_coding retained_intron 8.732579 4.966933 11.07937 0.3921004 0.3984612 1.155849 0.4483428 0.2211991 0.5643156 0.02227634 0.06287803 1.3127083 0.05095417 0.04566667 0.05136667 0.00570000 9.315359e-01 1.354036e-07 TRUE TRUE
ENST00000617312 ENSG00000177463 HEK293_OSMI2_2hA HEK293_TMG_2hB NR2C2 protein_coding protein_coding 8.732579 4.966933 11.07937 0.3921004 0.3984612 1.155849 0.5431920 0.0000000 1.6923199 0.00000000 0.84779816 7.4113584 0.05398333 0.00000000 0.14750000 0.14750000 2.587900e-01 1.354036e-07 FALSE TRUE
MSTRG.22528.5 ENSG00000177463 HEK293_OSMI2_2hA HEK293_TMG_2hB NR2C2 protein_coding   8.732579 4.966933 11.07937 0.3921004 0.3984612 1.155849 1.0010624 0.5989456 1.2316222 0.30220542 0.62128915 1.0278410 0.10464583 0.11960000 0.10726667 -0.01233333 9.998496e-01 1.354036e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000177463 E001 0.0000000       3 14947583 14947583 1 +      
ENSG00000177463 E002 7.2360176 0.0749176370 0.302907730 0.444826011 3 14947584 14947704 121 + 0.977 0.768 -0.803
ENSG00000177463 E003 7.0204200 0.0277575837 0.528021392 0.660940803 3 14947705 14947728 24 + 0.929 0.828 -0.384
ENSG00000177463 E004 13.3721759 0.0024535515 0.003068862 0.010469022 3 14947729 14947836 108 + 1.241 0.928 -1.135
ENSG00000177463 E005 8.8702154 0.0019360405 0.001337742 0.005102401 3 14947837 14947848 12 + 1.100 0.695 -1.551
ENSG00000177463 E006 11.4981458 0.0015503256 0.002558874 0.008938673 3 14947849 14947871 23 + 1.190 0.854 -1.236
ENSG00000177463 E007 14.3083663 0.0015315615 0.319458506 0.462539020 3 14947872 14947906 35 + 1.209 1.109 -0.358
ENSG00000177463 E008 0.1817044 0.0397448633 0.266039681   3 14948632 14948727 96 + 0.000 0.158 10.236
ENSG00000177463 E009 1.5154904 0.0130651482 0.737465030 0.827627013 3 14949239 14949306 68 + 0.430 0.365 -0.361
ENSG00000177463 E010 6.2889328 0.0037214587 0.437915532 0.579799589 3 14959157 14959196 40 + 0.903 0.797 -0.411
ENSG00000177463 E011 11.1466828 0.0015331183 0.396851555 0.540612059 3 14959536 14959641 106 + 1.047 1.123 0.278
ENSG00000177463 E012 0.0000000       3 14962407 14962408 2 +      
ENSG00000177463 E013 12.1186660 0.0050268591 0.194860449 0.319824366 3 14962409 14962492 84 + 1.056 1.178 0.438
ENSG00000177463 E014 25.1212583 0.0263808246 0.700688248 0.800103515 3 15003876 15003914 39 + 1.392 1.427 0.121
ENSG00000177463 E015 39.0003595 0.0178515368 0.718330924 0.813461945 3 15003915 15003986 72 + 1.582 1.606 0.083
ENSG00000177463 E016 13.7958305 0.0037043345 0.549652371 0.679358239 3 15004557 15004613 57 + 1.176 1.110 -0.236
ENSG00000177463 E017 74.1067321 0.0055835938 0.368435290 0.512850553 3 15013589 15013789 201 + 1.847 1.889 0.142
ENSG00000177463 E018 59.9180885 0.0019630343 0.524544212 0.658039143 3 15016152 15016254 103 + 1.763 1.787 0.081
ENSG00000177463 E019 84.0128972 0.0017940490 0.597956947 0.719633770 3 15020753 15020932 180 + 1.914 1.929 0.050
ENSG00000177463 E020 0.0000000       3 15020933 15020935 3 +      
ENSG00000177463 E021 80.8595793 0.0003764508 0.999887403 1.000000000 3 15023200 15023347 148 + 1.906 1.898 -0.027
ENSG00000177463 E022 68.5235087 0.0005242934 0.369010057 0.513439351 3 15024115 15024208 94 + 1.819 1.850 0.105
ENSG00000177463 E023 9.5139132 0.0122574118 0.002361393 0.008342510 3 15025565 15027009 1445 + 0.847 1.189 1.261
ENSG00000177463 E024 84.5742779 0.0010725404 0.774700243 0.854793571 3 15028586 15028719 134 + 1.920 1.924 0.013
ENSG00000177463 E025 86.4806413 0.0003395929 0.406504454 0.550140597 3 15030275 15030452 178 + 1.946 1.906 -0.136
ENSG00000177463 E026 49.9011889 0.0009495060 0.737250561 0.827477380 3 15032379 15032426 48 + 1.703 1.677 -0.087
ENSG00000177463 E027 53.3792498 0.0004329597 0.799329319 0.872009430 3 15032427 15032500 74 + 1.725 1.730 0.016
ENSG00000177463 E028 3.0440841 0.0601158841 0.024524987 0.060900033 3 15034324 15034652 329 + 0.728 0.274 -2.307
ENSG00000177463 E029 2.0120693 0.0550069776 0.033633913 0.079032583 3 15034653 15034669 17 + 0.591 0.159 -2.718
ENSG00000177463 E030 68.6854600 0.0003804776 0.042061008 0.094839641 3 15034670 15034809 140 + 1.799 1.879 0.268
ENSG00000177463 E031 57.7300334 0.0004520192 0.006507684 0.019942132 3 15038000 15038137 138 + 1.709 1.828 0.402
ENSG00000177463 E032 12.9630906 0.0016192811 0.690857631 0.792434780 3 15038138 15039121 984 + 1.154 1.109 -0.163
ENSG00000177463 E033 58.5274737 0.0004217736 0.281156035 0.421043969 3 15039122 15039227 106 + 1.750 1.792 0.143
ENSG00000177463 E034 1103.7931763 0.0024653882 0.253199726 0.389698014 3 15042834 15049273 6440 + 3.044 3.020 -0.078
ENSG00000177463 E035 1.7221843 0.0081333665 0.026380836 0.064711971 3 15053361 15053450 90 + 0.236 0.610 2.087
ENSG00000177463 E036 1.4632625 0.4361227375 0.243532309 0.378325901 3 15053451 15053600 150 + 0.235 0.523 1.699