ENSG00000177427

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000323019 ENSG00000177427 HEK293_OSMI2_2hA HEK293_TMG_2hB MIEF2 protein_coding protein_coding 12.65967 16.79512 8.224788 0.9719128 0.6080958 -1.029097 6.9899733 10.084841 4.0919216 0.6896158 0.4187401 -1.2992463 0.54688750 0.60000000 0.50166667 -0.09833333 4.385868e-01 2.267157e-07 FALSE  
ENST00000395703 ENSG00000177427 HEK293_OSMI2_2hA HEK293_TMG_2hB MIEF2 protein_coding protein_coding 12.65967 16.79512 8.224788 0.9719128 0.6080958 -1.029097 2.9227151 3.114906 2.1308493 0.5432936 0.3321665 -0.5456293 0.23758333 0.18340000 0.25610000 0.07270000 2.951436e-01 2.267157e-07 FALSE  
ENST00000395704 ENSG00000177427 HEK293_OSMI2_2hA HEK293_TMG_2hB MIEF2 protein_coding protein_coding 12.65967 16.79512 8.224788 0.9719128 0.6080958 -1.029097 0.5766025 1.830071 0.3211715 0.2238917 0.1996653 -2.4741109 0.04281250 0.11113333 0.04120000 -0.06993333 3.475607e-01 2.267157e-07 FALSE  
ENST00000395706 ENSG00000177427 HEK293_OSMI2_2hA HEK293_TMG_2hB MIEF2 protein_coding protein_coding 12.65967 16.79512 8.224788 0.9719128 0.6080958 -1.029097 0.2359934 0.000000 0.4027197 0.0000000 0.1046971 5.3670904 0.02047917 0.00000000 0.05006667 0.05006667 2.267157e-07 2.267157e-07 FALSE  
ENST00000578621 ENSG00000177427 HEK293_OSMI2_2hA HEK293_TMG_2hB MIEF2 protein_coding protein_coding 12.65967 16.79512 8.224788 0.9719128 0.6080958 -1.029097 1.5474354 1.659981 1.1694205 0.3211536 0.3078441 -0.5017531 0.12688750 0.09916667 0.13846667 0.03930000 5.769838e-01 2.267157e-07 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000177427 E001 3.9895810 0.2733130218 0.420674956 0.56386141 17 18260597 18260603 7 + 0.488 0.681 0.872
ENSG00000177427 E002 5.8600585 0.1840231393 0.488375363 0.62595138 17 18260604 18260615 12 + 0.666 0.808 0.579
ENSG00000177427 E003 13.0562829 0.0049573880 0.650392232 0.76144569 17 18260616 18260644 29 + 1.037 1.100 0.228
ENSG00000177427 E004 19.3541005 0.0013007217 0.681998048 0.78557348 17 18260645 18260660 16 + 1.207 1.257 0.176
ENSG00000177427 E005 18.2543354 0.0013333723 0.778683551 0.85760033 17 18260661 18260661 1 + 1.193 1.232 0.138
ENSG00000177427 E006 24.5745914 0.0009411236 0.974307349 0.98797461 17 18260662 18260673 12 + 1.329 1.345 0.057
ENSG00000177427 E007 54.1418041 0.0004845856 0.824529582 0.88945795 17 18260674 18260737 64 + 1.651 1.677 0.089
ENSG00000177427 E008 2.0210480 0.0365075192 0.986861876 0.99584009 17 18261110 18261161 52 + 0.409 0.422 0.070
ENSG00000177427 E009 93.4686061 0.0003890100 0.699115404 0.79894144 17 18262714 18262867 154 + 1.881 1.911 0.102
ENSG00000177427 E010 0.5212538 0.0212303377 0.812338946 0.88097807 17 18262868 18263085 218 + 0.182 0.148 -0.358
ENSG00000177427 E011 81.3676123 0.0004407353 0.762285382 0.84589379 17 18263086 18263181 96 + 1.844 1.845 0.004
ENSG00000177427 E012 74.5157565 0.0039817279 0.737945417 0.82796658 17 18263182 18263248 67 + 1.810 1.806 -0.012
ENSG00000177427 E013 16.3341530 0.0243525668 0.031564043 0.07500236 17 18263249 18263415 167 + 1.329 1.104 -0.795
ENSG00000177427 E014 15.6655881 0.0012824078 0.033656909 0.07907289 17 18263416 18263546 131 + 1.284 1.107 -0.629
ENSG00000177427 E015 10.3428120 0.0018112129 0.100312138 0.19095684 17 18263673 18263709 37 + 1.113 0.951 -0.596
ENSG00000177427 E016 38.6071906 0.0006742086 0.057346312 0.12206839 17 18263710 18263712 3 + 1.603 1.501 -0.348
ENSG00000177427 E017 74.9425271 0.0024602704 0.114711629 0.21241312 17 18263713 18263783 71 + 1.860 1.796 -0.214
ENSG00000177427 E018 360.0849282 0.0002825014 0.007954673 0.02366206 17 18263784 18264764 981 + 2.515 2.479 -0.119
ENSG00000177427 E019 283.3476169 0.0056201519 0.179279170 0.30022011 17 18264765 18265245 481 + 2.334 2.404 0.233
ENSG00000177427 E020 249.6535705 0.0051214718 0.018011038 0.04715697 17 18265246 18266552 1307 + 2.248 2.357 0.364