ENSG00000177302

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000321105 ENSG00000177302 HEK293_OSMI2_2hA HEK293_TMG_2hB TOP3A protein_coding protein_coding 23.06479 23.96741 21.85585 1.588875 0.5796907 -0.1329961 11.2015428 15.1985676 8.4679968 0.20321111 0.1448461 -0.8430890 0.48095000 0.63860000 0.38830000 -0.25030000 2.412974e-05 3.187785e-09 FALSE TRUE
ENST00000469739 ENSG00000177302 HEK293_OSMI2_2hA HEK293_TMG_2hB TOP3A protein_coding retained_intron 23.06479 23.96741 21.85585 1.588875 0.5796907 -0.1329961 1.1934561 1.3689689 0.8643108 0.28266213 0.2072357 -0.6573718 0.05147083 0.05920000 0.03940000 -0.01980000 6.711738e-01 3.187785e-09 FALSE TRUE
ENST00000542570 ENSG00000177302 HEK293_OSMI2_2hA HEK293_TMG_2hB TOP3A protein_coding protein_coding 23.06479 23.96741 21.85585 1.588875 0.5796907 -0.1329961 1.9523130 1.2469970 1.6817630 1.24699695 0.8496449 0.4285464 0.08650000 0.04646667 0.07513333 0.02866667 5.526458e-01 3.187785e-09 FALSE TRUE
ENST00000580095 ENSG00000177302 HEK293_OSMI2_2hA HEK293_TMG_2hB TOP3A protein_coding protein_coding 23.06479 23.96741 21.85585 1.588875 0.5796907 -0.1329961 0.6246826 0.0000000 2.0266847 0.00000000 0.9755401 7.6700789 0.02952917 0.00000000 0.09480000 0.09480000 8.002574e-07 3.187785e-09 FALSE TRUE
ENST00000583804 ENSG00000177302 HEK293_OSMI2_2hA HEK293_TMG_2hB TOP3A protein_coding retained_intron 23.06479 23.96741 21.85585 1.588875 0.5796907 -0.1329961 1.1582986 0.3589176 1.7491588 0.06873333 0.1603950 2.2535151 0.05080833 0.01473333 0.08030000 0.06556667 3.187785e-09 3.187785e-09 FALSE FALSE
ENST00000584582 ENSG00000177302 HEK293_OSMI2_2hA HEK293_TMG_2hB TOP3A protein_coding nonsense_mediated_decay 23.06479 23.96741 21.85585 1.588875 0.5796907 -0.1329961 1.9212265 2.9618006 0.8236529 1.15397102 0.4195086 -1.8338187 0.07953750 0.12273333 0.03850000 -0.08423333 5.180326e-01 3.187785e-09 FALSE TRUE
MSTRG.13793.9 ENSG00000177302 HEK293_OSMI2_2hA HEK293_TMG_2hB TOP3A protein_coding   23.06479 23.96741 21.85585 1.588875 0.5796907 -0.1329961 1.3418697 1.1877344 1.3608122 0.47522147 0.1960297 0.1947229 0.05945000 0.04983333 0.06270000 0.01286667 7.855831e-01 3.187785e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000177302 E001 13.0722721 0.0538343088 5.329805e-01 6.652489e-01 17 18271404 18271427 24 - 1.067 1.166 0.355
ENSG00000177302 E002 48.9709451 0.0220265914 1.783550e-02 4.678530e-02 17 18271428 18271571 144 - 1.518 1.746 0.776
ENSG00000177302 E003 50.3732542 0.0115778116 1.506453e-03 5.657060e-03 17 18271572 18271614 43 - 1.512 1.763 0.853
ENSG00000177302 E004 57.3790621 0.0062239270 1.136646e-04 5.846319e-04 17 18271615 18271673 59 - 1.564 1.819 0.866
ENSG00000177302 E005 421.0456232 0.0053483238 7.475219e-06 5.110823e-05 17 18271674 18272837 1164 - 2.475 2.658 0.608
ENSG00000177302 E006 206.2457108 0.0003302391 2.412735e-02 6.007562e-02 17 18272838 18273243 406 - 2.250 2.317 0.223
ENSG00000177302 E007 132.2582241 0.0002796483 4.344204e-02 9.736467e-02 17 18273244 18273336 93 - 2.054 2.126 0.243
ENSG00000177302 E008 271.9452951 0.0001815105 2.688497e-03 9.339635e-03 17 18273337 18273920 584 - 2.361 2.437 0.251
ENSG00000177302 E009 40.9104746 0.0217713938 4.034880e-02 9.166408e-02 17 18273921 18273966 46 - 1.465 1.656 0.651
ENSG00000177302 E010 110.1804259 0.0003079903 6.421963e-05 3.520826e-04 17 18273967 18274227 261 - 1.914 2.067 0.514
ENSG00000177302 E011 407.6018846 0.0016599071 4.147394e-08 4.547467e-07 17 18274228 18274723 496 - 2.483 2.636 0.508
ENSG00000177302 E012 155.5570305 0.0004063211 7.412921e-07 6.334486e-06 17 18274724 18274794 71 - 2.057 2.220 0.545
ENSG00000177302 E013 102.2494683 0.0006529256 1.577813e-05 1.001749e-04 17 18274795 18274803 9 - 1.866 2.042 0.593
ENSG00000177302 E014 245.2204319 0.0003875287 3.100459e-05 1.838527e-04 17 18274804 18274980 177 - 2.291 2.403 0.374
ENSG00000177302 E015 501.6368793 0.0003518030 1.257919e-09 1.829066e-08 17 18277675 18278357 683 - 2.596 2.714 0.392
ENSG00000177302 E016 0.9317929 0.0543305299 1.835380e-01 3.056471e-01 17 18280175 18280535 361 - 0.421 0.181 -1.667
ENSG00000177302 E017 161.5172932 0.0002332356 1.293780e-03 4.955273e-03 17 18280536 18280658 123 - 2.115 2.218 0.343
ENSG00000177302 E018 178.9192812 0.0002430290 2.760563e-01 4.154627e-01 17 18282698 18282790 93 - 2.205 2.242 0.123
ENSG00000177302 E019 145.0332136 0.0002330392 4.672657e-01 6.067769e-01 17 18282791 18282841 51 - 2.150 2.135 -0.053
ENSG00000177302 E020 240.1984715 0.0003025903 1.402416e-02 3.828016e-02 17 18285142 18285307 166 - 2.392 2.339 -0.177
ENSG00000177302 E021 112.9542108 0.0005289492 7.628400e-01 8.463506e-01 17 18285407 18285427 21 - 2.036 2.032 -0.014
ENSG00000177302 E022 176.9096416 0.0002719475 8.134043e-02 1.616510e-01 17 18285428 18285520 93 - 2.253 2.211 -0.141
ENSG00000177302 E023 2.5087914 0.0061017191 8.992706e-01 9.397325e-01 17 18290540 18290556 17 - 0.539 0.522 -0.077
ENSG00000177302 E024 172.3114484 0.0096094595 1.101952e-02 3.120688e-02 17 18290557 18290686 130 - 2.298 2.164 -0.448
ENSG00000177302 E025 198.8293465 0.0001907722 6.025063e-07 5.246465e-06 17 18290842 18291027 186 - 2.352 2.230 -0.406
ENSG00000177302 E026 196.9592048 0.0004506784 1.029484e-02 2.949276e-02 17 18292645 18292852 208 - 2.313 2.250 -0.211
ENSG00000177302 E027 133.1170253 0.0009258573 2.672615e-04 1.246324e-03 17 18294703 18294785 83 - 2.176 2.060 -0.390
ENSG00000177302 E028 125.2108026 0.0002907142 1.978593e-05 1.228334e-04 17 18299559 18299633 75 - 2.162 2.031 -0.439
ENSG00000177302 E029 134.5572651 0.0005082153 1.227353e-07 1.225587e-06 17 18301885 18301985 101 - 2.209 2.047 -0.542
ENSG00000177302 E030 158.9810174 0.0034164008 2.587680e-04 1.211667e-03 17 18302264 18302434 171 - 2.266 2.130 -0.453
ENSG00000177302 E031 85.6900304 0.0097457410 1.590870e-01 2.738948e-01 17 18302580 18302603 24 - 1.963 1.887 -0.256
ENSG00000177302 E032 142.0587655 0.0017918061 1.852085e-04 9.000408e-04 17 18302604 18302685 82 - 2.210 2.084 -0.423
ENSG00000177302 E033 97.6312220 0.0003915779 1.211850e-06 9.887223e-06 17 18302686 18302723 38 - 2.074 1.903 -0.574
ENSG00000177302 E034 14.3093748 0.0019195669 7.079317e-12 1.511502e-10 17 18302724 18303090 367 - 1.460 0.842 -2.225
ENSG00000177302 E035 153.4393678 0.0021342312 5.826443e-06 4.085886e-05 17 18305112 18305220 109 - 2.261 2.106 -0.517
ENSG00000177302 E036 3.1117221 0.0051569038 1.056380e-03 4.154535e-03 17 18306550 18306587 38 - 0.854 0.360 -2.247
ENSG00000177302 E037 8.9871207 0.0020700791 1.822864e-06 1.430792e-05 17 18306588 18306890 303 - 1.240 0.732 -1.897
ENSG00000177302 E038 107.5971980 0.0011459400 2.472696e-03 8.682138e-03 17 18306891 18306933 43 - 2.078 1.971 -0.360
ENSG00000177302 E039 92.8695409 0.0033620730 8.563914e-03 2.520010e-02 17 18306934 18306962 29 - 2.019 1.907 -0.379
ENSG00000177302 E040 65.2777126 0.0041923829 7.529108e-03 2.258768e-02 17 18306963 18306966 4 - 1.880 1.745 -0.458
ENSG00000177302 E041 6.5699221 0.0025316551 3.656908e-02 8.465489e-02 17 18306967 18307159 193 - 1.000 0.753 -0.951
ENSG00000177302 E042 3.1270711 0.0052430040 7.423247e-01 8.312406e-01 17 18307473 18307525 53 - 0.631 0.585 -0.203
ENSG00000177302 E043 1.2147488 0.0113697263 8.128733e-01 8.813594e-01 17 18307526 18307531 6 - 0.348 0.309 -0.245
ENSG00000177302 E044 1.8038586 0.0096538347 9.695382e-02 1.858299e-01 17 18307532 18307570 39 - 0.587 0.309 -1.468
ENSG00000177302 E045 4.6404852 0.0033889188 1.509694e-01 2.629731e-01 17 18308313 18308350 38 - 0.854 0.665 -0.762
ENSG00000177302 E046 91.4759061 0.0027588056 3.112899e-03 1.059806e-02 17 18308351 18308412 62 - 2.019 1.895 -0.417
ENSG00000177302 E047 51.7258803 0.0006244345 4.399552e-04 1.927928e-03 17 18308413 18308424 12 - 1.801 1.633 -0.570
ENSG00000177302 E048 0.5149242 0.2071265135 9.085754e-01 9.459052e-01 17 18308682 18308881 200 - 0.150 0.182 0.338
ENSG00000177302 E049 90.4347200 0.0003435417 3.918651e-07 3.538665e-06 17 18308882 18308941 60 - 2.049 1.865 -0.620
ENSG00000177302 E050 134.4065560 0.0085762498 6.295868e-02 1.316410e-01 17 18314599 18315007 409 - 2.165 2.075 -0.303