ENSG00000177169

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000321867 ENSG00000177169 HEK293_OSMI2_2hA HEK293_TMG_2hB ULK1 protein_coding protein_coding 21.18216 27.56214 15.07206 2.519548 0.4464583 -0.8703781 9.266060 10.305708 7.0883312 1.6269742 0.4107448 -0.53929100 0.4484125 0.37326667 0.46956667 0.0963000 0.3840204401 0.0008627371 FALSE TRUE
ENST00000540568 ENSG00000177169 HEK293_OSMI2_2hA HEK293_TMG_2hB ULK1 protein_coding retained_intron 21.18216 27.56214 15.07206 2.519548 0.4464583 -0.8703781 2.285588 2.224765 2.2622824 0.1455987 0.1058450 0.02401899 0.1137708 0.08126667 0.15056667 0.0693000 0.0008627371 0.0008627371 FALSE TRUE
ENST00000540647 ENSG00000177169 HEK293_OSMI2_2hA HEK293_TMG_2hB ULK1 protein_coding retained_intron 21.18216 27.56214 15.07206 2.519548 0.4464583 -0.8703781 7.685625 12.648404 4.2253444 1.2554594 0.3776086 -1.57954437 0.3404792 0.46183333 0.27996667 -0.1818667 0.0141646677 0.0008627371 FALSE TRUE
MSTRG.8325.2 ENSG00000177169 HEK293_OSMI2_2hA HEK293_TMG_2hB ULK1 protein_coding   21.18216 27.56214 15.07206 2.519548 0.4464583 -0.8703781 1.136875 1.988698 0.7958675 0.7634728 0.4064315 -1.31044614 0.0558750 0.06933333 0.05423333 -0.0151000 0.7853453890 0.0008627371 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000177169 E001 17.9196804 0.0016130059 2.245118e-03 7.983536e-03 12 131894622 131894778 157 + 1.378 1.154 -0.788
ENSG00000177169 E002 53.4023571 0.0070654017 2.753184e-03 9.531320e-03 12 131894779 131895112 334 + 1.795 1.639 -0.527
ENSG00000177169 E003 67.5561179 0.0075906272 2.248260e-02 5.668962e-02 12 131895601 131895693 93 + 1.867 1.762 -0.354
ENSG00000177169 E004 61.7618863 0.0041377259 6.660250e-03 2.033997e-02 12 131895783 131895824 42 + 1.838 1.719 -0.400
ENSG00000177169 E005 2.8940598 0.1104399365 2.190636e-01 3.492610e-01 12 131906490 131906891 402 + 0.688 0.478 -0.950
ENSG00000177169 E006 51.8788250 0.0004916418 5.539155e-03 1.738875e-02 12 131906892 131906924 33 + 1.760 1.648 -0.382
ENSG00000177169 E007 62.2456188 0.0038295050 7.647381e-02 1.538564e-01 12 131907495 131907531 37 + 1.810 1.743 -0.226
ENSG00000177169 E008 0.1451727 0.0436356640 2.637815e-01   12 131908619 131908643 25 + 0.156 0.000 -20.103
ENSG00000177169 E009 101.6289162 0.0027329757 2.017290e-03 7.280113e-03 12 131908644 131908817 174 + 2.044 1.940 -0.350
ENSG00000177169 E010 64.6663285 0.0005148811 7.947795e-04 3.241101e-03 12 131908898 131908971 74 + 1.862 1.736 -0.423
ENSG00000177169 E011 69.8879323 0.0054338423 3.291314e-01 4.726359e-01 12 131909136 131909237 102 + 1.835 1.810 -0.085
ENSG00000177169 E012 65.4802509 0.0005495572 2.146212e-02 5.456384e-02 12 131909775 131909833 59 + 1.840 1.763 -0.262
ENSG00000177169 E013 80.9919558 0.0034508450 5.529485e-03 1.736415e-02 12 131909919 131910001 83 + 1.944 1.839 -0.350
ENSG00000177169 E014 67.6598272 0.0100492311 1.892241e-01 3.127600e-01 12 131910254 131910304 51 + 1.835 1.783 -0.174
ENSG00000177169 E015 84.8217556 0.0094031850 4.201427e-01 5.633672e-01 12 131910712 131910800 89 + 1.905 1.893 -0.041
ENSG00000177169 E016 99.2973062 0.0139025201 2.191485e-01 3.493435e-01 12 131911942 131912089 148 + 1.993 1.952 -0.137
ENSG00000177169 E017 57.6960422 0.0057434784 1.327825e-01 2.379778e-01 12 131913198 131913258 61 + 1.767 1.709 -0.196
ENSG00000177169 E018 73.4922269 0.0047369186 3.855189e-01 5.297163e-01 12 131913747 131913836 90 + 1.843 1.828 -0.052
ENSG00000177169 E019 98.4649622 0.0091272700 5.741643e-01 7.000193e-01 12 131914352 131914477 126 + 1.963 1.962 -0.004
ENSG00000177169 E020 49.3202604 0.0169262696 8.785057e-01 9.259655e-01 12 131915083 131915100 18 + 1.637 1.682 0.152
ENSG00000177169 E021 101.1326536 0.0042469075 2.909630e-01 4.317813e-01 12 131915101 131915231 131 + 1.915 1.998 0.280
ENSG00000177169 E022 85.0555878 0.0004663206 6.371111e-01 7.510581e-01 12 131915335 131915421 87 + 1.862 1.909 0.159
ENSG00000177169 E023 60.5626390 0.0131805058 8.695537e-01 9.201459e-01 12 131915891 131915942 52 + 1.734 1.759 0.087
ENSG00000177169 E024 112.5195911 0.0049875625 6.085962e-01 7.282926e-01 12 131915943 131916159 217 + 1.979 2.036 0.192
ENSG00000177169 E025 0.1515154 0.0426307004 2.639166e-01   12 131916160 131916397 238 + 0.156 0.000 -20.104
ENSG00000177169 E026 139.7016458 0.0002205429 5.265699e-02 1.138854e-01 12 131916398 131916591 194 + 2.046 2.137 0.303
ENSG00000177169 E027 123.5176861 0.0002500856 3.938229e-01 5.377194e-01 12 131916953 131917062 110 + 2.018 2.075 0.190
ENSG00000177169 E028 111.3635811 0.0003051593 1.144370e-01 2.120173e-01 12 131917411 131917554 144 + 1.956 2.041 0.284
ENSG00000177169 E029 21.6527339 0.0104813612 1.875537e-03 6.840214e-03 12 131918302 131918496 195 + 1.463 1.221 -0.844
ENSG00000177169 E030 132.1626852 0.0003010941 1.266822e-01 2.295247e-01 12 131918497 131918681 185 + 2.031 2.109 0.264
ENSG00000177169 E031 86.3886990 0.0048727617 8.733827e-01 9.226350e-01 12 131919212 131919240 29 + 1.889 1.911 0.074
ENSG00000177169 E032 148.7408327 0.0002669712 8.763281e-01 9.246220e-01 12 131919241 131919384 144 + 2.122 2.144 0.076
ENSG00000177169 E033 2.8193015 0.0189849017 5.610155e-05 3.122013e-04 12 131919385 131919471 87 + 0.874 0.240 -3.138
ENSG00000177169 E034 141.6858575 0.0003088290 8.045082e-01 8.756415e-01 12 131919472 131919590 119 + 2.103 2.122 0.066
ENSG00000177169 E035 4.1630997 0.0097182145 7.681453e-03 2.298013e-02 12 131919591 131919978 388 + 0.898 0.538 -1.495
ENSG00000177169 E036 110.6431360 0.0003197711 5.007753e-01 6.369925e-01 12 131919979 131920066 88 + 1.977 2.028 0.174
ENSG00000177169 E037 111.3071059 0.0025089233 3.180235e-01 4.609445e-01 12 131920067 131920136 70 + 1.967 2.038 0.241
ENSG00000177169 E038 55.3492193 0.0004672969 1.165134e-10 2.033639e-09 12 131920137 131921099 963 + 1.887 1.607 -0.945
ENSG00000177169 E039 159.0655333 0.0011762644 4.633782e-01 6.031718e-01 12 131921100 131921235 136 + 2.133 2.186 0.177
ENSG00000177169 E040 1269.3896091 0.0075054541 2.911458e-03 1.000375e-02 12 131921306 131923150 1845 + 2.979 3.111 0.438