ENSG00000176953

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000320805 ENSG00000176953 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC2IP protein_coding protein_coding 33.94876 32.56768 33.23126 0.7290525 0.4227416 0.02909114 11.5622217 15.5152991 11.937010 2.0056352 0.3723007 -0.37797143 0.35232083 0.47453333 0.35910000 -0.115433333 2.634942e-01 3.862632e-05 FALSE TRUE
ENST00000565752 ENSG00000176953 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC2IP protein_coding retained_intron 33.94876 32.56768 33.23126 0.7290525 0.4227416 0.02909114 2.4279739 1.2553125 2.720337 0.3917944 0.1320030 1.10958540 0.07008333 0.03880000 0.08196667 0.043166667 1.597981e-01 3.862632e-05 FALSE TRUE
ENST00000568148 ENSG00000176953 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC2IP protein_coding protein_coding 33.94876 32.56768 33.23126 0.7290525 0.4227416 0.02909114 5.3377255 4.4450006 4.316246 0.1413794 0.2327618 -0.04231008 0.15359583 0.13680000 0.12986667 -0.006933333 8.671459e-01 3.862632e-05 FALSE TRUE
ENST00000568998 ENSG00000176953 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC2IP protein_coding retained_intron 33.94876 32.56768 33.23126 0.7290525 0.4227416 0.02909114 5.7129870 2.6287997 6.369137 0.3778804 0.5132581 1.27347934 0.16330000 0.08026667 0.19153333 0.111266667 4.953434e-04 3.862632e-05 FALSE FALSE
MSTRG.12371.1 ENSG00000176953 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC2IP protein_coding   33.94876 32.56768 33.23126 0.7290525 0.4227416 0.02909114 1.9587488 0.4903967 1.144607 0.2821628 0.3455088 1.20625777 0.05724167 0.01516667 0.03460000 0.019433333 5.584337e-01 3.862632e-05   FALSE
MSTRG.12371.11 ENSG00000176953 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC2IP protein_coding   33.94876 32.56768 33.23126 0.7290525 0.4227416 0.02909114 4.8284787 3.8704025 5.234752 0.5388994 0.5370879 0.43466828 0.13981250 0.11873333 0.15776667 0.039033333 4.576539e-01 3.862632e-05 FALSE TRUE
MSTRG.12371.4 ENSG00000176953 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC2IP protein_coding   33.94876 32.56768 33.23126 0.7290525 0.4227416 0.02909114 0.4323348 2.3944939 0.000000 1.5693438 0.0000000 -7.90958946 0.01350417 0.07576667 0.00000000 -0.075766667 3.862632e-05 3.862632e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000176953 E001 1.5112360 0.0202704184 6.650984e-01 7.728030e-01 16 28950694 28950806 113 + 0.447 0.392 -0.293
ENSG00000176953 E002 0.4417591 0.1043446289 7.645954e-01 8.476261e-01 16 28950807 28950834 28 + 0.133 0.199 0.697
ENSG00000176953 E003 0.2966881 0.0272339726 1.367217e-01   16 28950937 28950987 51 + 0.235 0.000 -15.253
ENSG00000176953 E004 3.2923056 0.0090315509 2.103577e-01 3.389701e-01 16 28950988 28951006 19 + 0.725 0.562 -0.704
ENSG00000176953 E005 19.3152051 0.0009655711 4.132913e-01 5.568010e-01 16 28951007 28951051 45 + 1.317 1.285 -0.114
ENSG00000176953 E006 45.3296393 0.0104591155 5.459854e-01 6.762352e-01 16 28951052 28951105 54 + 1.659 1.648 -0.036
ENSG00000176953 E007 205.0093432 0.0061866808 1.157967e-01 2.139434e-01 16 28951106 28951398 293 + 2.326 2.289 -0.125
ENSG00000176953 E008 151.0876846 0.0059683759 6.565096e-01 7.660512e-01 16 28952132 28952204 73 + 2.166 2.177 0.038
ENSG00000176953 E009 31.4785847 0.0057224867 1.633330e-02 4.346163e-02 16 28952205 28952643 439 + 1.576 1.437 -0.477
ENSG00000176953 E010 100.4337250 0.0003564241 4.102306e-02 9.292397e-02 16 28954565 28954566 2 + 2.022 1.982 -0.136
ENSG00000176953 E011 154.8928929 0.0002782742 2.471516e-02 6.129352e-02 16 28954567 28954605 39 + 2.203 2.170 -0.108
ENSG00000176953 E012 208.3560890 0.0001910511 2.923863e-02 7.046844e-02 16 28954606 28954682 77 + 2.324 2.303 -0.071
ENSG00000176953 E013 42.7348256 0.0012570127 8.998140e-12 1.888832e-10 16 28955249 28955404 156 + 1.801 1.454 -1.181
ENSG00000176953 E014 34.9908377 0.0204422276 2.599182e-04 1.216541e-03 16 28955920 28955977 58 + 1.699 1.395 -1.037
ENSG00000176953 E015 195.7568503 0.0017794262 3.733148e-01 5.177672e-01 16 28955978 28956058 81 + 2.282 2.287 0.015
ENSG00000176953 E016 248.0330372 0.0020783074 7.892822e-01 8.651189e-01 16 28956151 28956337 187 + 2.361 2.402 0.137
ENSG00000176953 E017 57.8529605 0.0158630726 1.315973e-08 1.586730e-07 16 28956338 28956712 375 + 1.952 1.547 -1.370
ENSG00000176953 E018 39.2726499 0.0011381740 2.643280e-02 6.481258e-02 16 28958390 28958582 193 + 1.648 1.554 -0.322
ENSG00000176953 E019 38.8007113 0.0005596091 1.463544e-04 7.313935e-04 16 28958583 28958716 134 + 1.688 1.508 -0.614
ENSG00000176953 E020 269.6907217 0.0002079280 1.333597e-01 2.387912e-01 16 28958717 28958861 145 + 2.382 2.448 0.220
ENSG00000176953 E021 285.0830826 0.0001978939 1.727227e-02 4.554942e-02 16 28958991 28959100 110 + 2.394 2.479 0.281
ENSG00000176953 E022 2312.2086047 0.0038861246 4.456784e-03 1.442065e-02 16 28963705 28967092 3388 + 3.307 3.387 0.266