ENSG00000176896

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000380600 ENSG00000176896 HEK293_OSMI2_2hA HEK293_TMG_2hB TCEANC protein_coding protein_coding 1.847253 0.876522 2.944544 0.1139211 0.1417824 1.736707 0.30390856 0.03399551 0.70302127 0.033995513 0.208351561 4.01851682 0.14416667 0.03560000 0.243366667 0.20776667 7.553430e-02 8.061236e-08 FALSE TRUE
ENST00000463321 ENSG00000176896 HEK293_OSMI2_2hA HEK293_TMG_2hB TCEANC protein_coding processed_transcript 1.847253 0.876522 2.944544 0.1139211 0.1417824 1.736707 0.12754463 0.03886436 0.22389455 0.038864363 0.127862749 2.25900366 0.06986667 0.04070000 0.073266667 0.03256667 8.599189e-01 8.061236e-08   FALSE
ENST00000467590 ENSG00000176896 HEK293_OSMI2_2hA HEK293_TMG_2hB TCEANC protein_coding nonsense_mediated_decay 1.847253 0.876522 2.944544 0.1139211 0.1417824 1.736707 0.14633864 0.11974044 0.11359835 0.006278747 0.002469809 -0.06996876 0.09856667 0.14413333 0.038666667 -0.10546667 2.461213e-02 8.061236e-08 TRUE TRUE
ENST00000544987 ENSG00000176896 HEK293_OSMI2_2hA HEK293_TMG_2hB TCEANC protein_coding protein_coding 1.847253 0.876522 2.944544 0.1139211 0.1417824 1.736707 0.08691986 0.04071670 0.03048502 0.040716701 0.030485022 -0.32507262 0.05429167 0.06243333 0.009466667 -0.05296667 7.896891e-01 8.061236e-08 FALSE TRUE
ENST00000696126 ENSG00000176896 HEK293_OSMI2_2hA HEK293_TMG_2hB TCEANC protein_coding protein_coding 1.847253 0.876522 2.944544 0.1139211 0.1417824 1.736707 0.49856634 0.00000000 0.88836520 0.000000000 0.099021260 6.48923014 0.19220833 0.00000000 0.300133333 0.30013333 8.061236e-08 8.061236e-08 FALSE FALSE
ENST00000696127 ENSG00000176896 HEK293_OSMI2_2hA HEK293_TMG_2hB TCEANC protein_coding protein_coding 1.847253 0.876522 2.944544 0.1139211 0.1417824 1.736707 0.07816820 0.20771594 0.20076187 0.207715939 0.117940608 -0.04683311 0.05662500 0.20313333 0.065500000 -0.13763333 9.757439e-01 8.061236e-08 FALSE TRUE
ENST00000696128 ENSG00000176896 HEK293_OSMI2_2hA HEK293_TMG_2hB TCEANC protein_coding protein_coding 1.847253 0.876522 2.944544 0.1139211 0.1417824 1.736707 0.31281398 0.27305787 0.47532728 0.052792742 0.237724734 0.77786092 0.19417083 0.33526667 0.169533333 -0.16573333 6.503723e-01 8.061236e-08 FALSE TRUE
ENST00000696129 ENSG00000176896 HEK293_OSMI2_2hA HEK293_TMG_2hB TCEANC protein_coding protein_coding 1.847253 0.876522 2.944544 0.1139211 0.1417824 1.736707 0.14817918 0.16243120 0.13706257 0.067712723 0.074021633 -0.22959076 0.12155833 0.17870000 0.046766667 -0.13193333 2.625274e-01 8.061236e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000176896 E001 0.6060617 0.8484601462 6.482919e-01 7.598272e-01 X 13653112 13653129 18 + 0.264 0.000 -10.719
ENSG00000176896 E002 0.7533107 0.2633149411 2.343072e-01 3.675299e-01 X 13653130 13653140 11 + 0.309 0.000 -13.224
ENSG00000176896 E003 3.9461390 0.0040594697 1.142841e-01 2.117856e-01 X 13653141 13653178 38 + 0.743 0.485 -1.145
ENSG00000176896 E004 5.1423401 0.0036155063 1.686906e-01 2.864641e-01 X 13653179 13653185 7 + 0.818 0.611 -0.855
ENSG00000176896 E005 5.1423401 0.0036155063 1.686906e-01 2.864641e-01 X 13653186 13653188 3 + 0.818 0.611 -0.855
ENSG00000176896 E006 0.2966881 0.0290785164 6.035195e-01   X 13653189 13653218 30 + 0.150 0.000 -12.213
ENSG00000176896 E007 0.7448917 0.0620995942 1.523700e-01 2.649110e-01 X 13653219 13653312 94 + 0.308 0.000 -13.349
ENSG00000176896 E008 8.6143385 0.0024929295 7.407272e-02 1.500437e-01 X 13655289 13655373 85 + 1.012 0.788 -0.855
ENSG00000176896 E009 7.1957591 0.0030716202 1.615098e-01 2.770738e-01 X 13659652 13659768 117 + 0.936 0.750 -0.724
ENSG00000176896 E010 5.0813930 0.0036755638 5.744264e-01 7.002409e-01 X 13660866 13661027 162 + 0.789 0.708 -0.330
ENSG00000176896 E011 2.7658299 0.0057006528 5.416252e-01 6.725874e-01 X 13661028 13661030 3 + 0.590 0.485 -0.493
ENSG00000176896 E012 6.7654393 0.0024658071 2.150457e-01 3.444931e-01 X 13661031 13661122 92 + 0.915 0.750 -0.645
ENSG00000176896 E013 17.6123557 0.0019333477 1.131037e-01 2.100639e-01 X 13662501 13662743 243 + 1.282 1.141 -0.501
ENSG00000176896 E014 32.0286635 0.0006451296 2.980404e-01 4.394271e-01 X 13662744 13663468 725 + 1.516 1.450 -0.225
ENSG00000176896 E015 7.5206229 0.0025183251 7.427762e-01 8.315953e-01 X 13663469 13663560 92 + 0.926 0.885 -0.156
ENSG00000176896 E016 41.1666348 0.0108647759 4.155785e-01 5.589483e-01 X 13663561 13664429 869 + 1.586 1.640 0.183
ENSG00000176896 E017 31.4434955 0.0008624403 7.713648e-07 6.566288e-06 X 13664430 13665409 980 + 1.378 1.659 0.962
ENSG00000176896 E018 1.5522376 0.0281248659 5.500923e-01 6.797600e-01 X 13666076 13666316 241 + 0.423 0.307 -0.685
ENSG00000176896 E019 2.6743286 0.5442780827 7.507463e-01 8.373464e-01 X 13666317 13666470 154 + 0.543 0.513 -0.140
ENSG00000176896 E020 4.8143397 0.0059260788 3.418496e-01 4.857956e-01 X 13667684 13668383 700 + 0.692 0.823 0.526
ENSG00000176896 E021 1.2867695 0.0146531414 2.097499e-01 3.382491e-01 X 13680489 13680598 110 + 0.261 0.485 1.314
ENSG00000176896 E022 2.6205660 0.0058018033 2.196768e-02 5.560663e-02 X 13681139 13681964 826 + 0.388 0.750 1.676