ENSG00000176853

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000334705 ENSG00000176853 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM91A1 protein_coding protein_coding 11.92259 3.493174 20.98071 0.6796126 0.3770053 2.583017 5.2463991 2.40120003 13.6592229 0.43206277 1.1080067 2.503108 0.55099167 0.69096667 0.65230000 -0.03866667 8.786061e-01 3.074896e-07 FALSE TRUE
ENST00000518333 ENSG00000176853 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM91A1 protein_coding retained_intron 11.92259 3.493174 20.98071 0.6796126 0.3770053 2.583017 0.2326704 0.83202357 0.3194098 0.33817672 0.1133071 -1.353977 0.05481667 0.22210000 0.01533333 -0.20676667 3.074896e-07 3.074896e-07 FALSE FALSE
ENST00000519721 ENSG00000176853 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM91A1 protein_coding nonsense_mediated_decay 11.92259 3.493174 20.98071 0.6796126 0.3770053 2.583017 1.2370512 0.00000000 0.2744549 0.00000000 0.1690909 4.830128 0.06729583 0.00000000 0.01283333 0.01283333 6.153257e-01 3.074896e-07 FALSE TRUE
ENST00000521166 ENSG00000176853 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM91A1 protein_coding protein_coding 11.92259 3.493174 20.98071 0.6796126 0.3770053 2.583017 0.9479012 0.04947511 1.8938125 0.01020571 0.1603351 5.000462 0.05087500 0.01583333 0.09056667 0.07473333 2.197619e-04 3.074896e-07 FALSE TRUE
MSTRG.32055.2 ENSG00000176853 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM91A1 protein_coding   11.92259 3.493174 20.98071 0.6796126 0.3770053 2.583017 2.0573301 0.00000000 2.1109700 0.00000000 1.0649264 7.728580 0.09783750 0.00000000 0.10010000 0.10010000 3.966080e-01 3.074896e-07 FALSE TRUE
MSTRG.32055.5 ENSG00000176853 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM91A1 protein_coding   11.92259 3.493174 20.98071 0.6796126 0.3770053 2.583017 1.8249758 0.08933255 2.3557808 0.04797262 0.7844216 4.573906 0.09962500 0.03193333 0.11120000 0.07926667 2.510046e-01 3.074896e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000176853 E001 1.3380404 0.3209041748 3.945305e-01 5.383575e-01 8 123768439 123768450 12 + 0.291 0.000 -12.501
ENSG00000176853 E002 2.5119937 0.0255282437 6.191469e-02 1.298407e-01 8 123768451 123768455 5 + 0.447 0.000 -13.324
ENSG00000176853 E003 47.6776330 0.0020244106 6.265359e-07 5.436262e-06 8 123768456 123768645 190 + 1.521 1.204 -1.104
ENSG00000176853 E004 55.1102090 0.0005936599 1.362277e-10 2.351520e-09 8 123768646 123768774 129 + 1.588 1.146 -1.538
ENSG00000176853 E005 1.8102039 0.0077700138 6.053743e-01 7.257802e-01 8 123772959 123773032 74 + 0.336 0.301 -0.222
ENSG00000176853 E006 28.1742657 0.0025152234 7.557935e-08 7.857689e-07 8 123774080 123774081 2 + 1.314 0.699 -2.293
ENSG00000176853 E007 51.9291538 0.0004435752 5.967190e-07 5.200176e-06 8 123774082 123774164 83 + 1.555 1.279 -0.954
ENSG00000176853 E008 74.2897296 0.0005725828 3.117191e-09 4.215450e-08 8 123775147 123775298 152 + 1.708 1.431 -0.945
ENSG00000176853 E009 49.8612981 0.0009817919 6.225478e-06 4.337298e-05 8 123777265 123777322 58 + 1.538 1.301 -0.819
ENSG00000176853 E010 48.3982315 0.0007432988 1.440779e-05 9.225691e-05 8 123778025 123778092 68 + 1.523 1.301 -0.767
ENSG00000176853 E011 68.4509272 0.0044257551 7.808690e-07 6.638841e-06 8 123778659 123778772 114 + 1.671 1.415 -0.875
ENSG00000176853 E012 54.8843686 0.0003980864 2.779371e-08 3.149561e-07 8 123779985 123780026 42 + 1.581 1.255 -1.126
ENSG00000176853 E013 57.6542400 0.0003962465 1.400266e-08 1.680133e-07 8 123780027 123780075 49 + 1.602 1.279 -1.116
ENSG00000176853 E014 0.1451727 0.0440066413 8.465139e-01   8 123780076 123780082 7 + 0.044 0.000 -9.236
ENSG00000176853 E015 45.1742433 0.0004543205 3.242034e-05 1.912720e-04 8 123780480 123780542 63 + 1.493 1.278 -0.742
ENSG00000176853 E016 67.0174402 0.0004130798 1.710583e-04 8.396333e-04 8 123784470 123784576 107 + 1.648 1.544 -0.354
ENSG00000176853 E017 0.0000000       8 123784577 123784795 219 +      
ENSG00000176853 E018 53.3821396 0.0013593873 4.200566e-02 9.473497e-02 8 123785081 123785119 39 + 1.540 1.544 0.013
ENSG00000176853 E019 80.9628120 0.0008511842 7.751767e-04 3.169827e-03 8 123785629 123785741 113 + 1.724 1.672 -0.178
ENSG00000176853 E020 0.1515154 0.0443146241 8.486426e-01   8 123786004 123786151 148 + 0.044 0.000 -9.237
ENSG00000176853 E021 93.7844204 0.0004067741 4.242742e-04 1.867463e-03 8 123786495 123786610 116 + 1.789 1.748 -0.139
ENSG00000176853 E022 102.0585967 0.0011017267 8.721249e-05 4.619512e-04 8 123787261 123787373 113 + 1.827 1.763 -0.218
ENSG00000176853 E023 84.5591117 0.0003436119 1.100564e-04 5.682086e-04 8 123787664 123787750 87 + 1.748 1.672 -0.259
ENSG00000176853 E024 78.7192712 0.0003095135 7.097898e-04 2.934113e-03 8 123789613 123789745 133 + 1.714 1.662 -0.175
ENSG00000176853 E025 67.2777438 0.0004054302 2.562842e-03 8.950719e-03 8 123798090 123798238 149 + 1.646 1.602 -0.149
ENSG00000176853 E026 86.7678339 0.0003046614 2.671297e-04 1.245770e-03 8 123799520 123799654 135 + 1.756 1.699 -0.195
ENSG00000176853 E027 81.3103522 0.0003270808 8.324539e-03 2.460387e-02 8 123799772 123799885 114 + 1.722 1.724 0.007
ENSG00000176853 E028 75.4984651 0.0003414573 8.302658e-04 3.367330e-03 8 123805267 123805339 73 + 1.697 1.643 -0.182
ENSG00000176853 E029 104.8816738 0.0017615344 1.192966e-02 3.336393e-02 8 123806080 123806229 150 + 1.828 1.845 0.057
ENSG00000176853 E030 88.8285802 0.0003249947 1.672824e-01 2.846647e-01 8 123808272 123808376 105 + 1.749 1.832 0.281
ENSG00000176853 E031 1.6422292 0.0577884896 6.926935e-01 7.938628e-01 8 123808759 123808892 134 + 0.314 0.300 -0.092
ENSG00000176853 E032 52.6625438 0.0006015551 3.959249e-01 5.397181e-01 8 123808893 123808906 14 + 1.525 1.623 0.333
ENSG00000176853 E033 49.3228117 0.0017472684 8.725158e-01 9.220066e-01 8 123808907 123808928 22 + 1.487 1.653 0.567
ENSG00000176853 E034 67.4804910 0.0004033877 6.868532e-01 7.892991e-01 8 123808929 123809016 88 + 1.618 1.799 0.614
ENSG00000176853 E035 63.9726369 0.0003795655 4.815333e-01 6.197702e-01 8 123810282 123810351 70 + 1.593 1.792 0.676
ENSG00000176853 E036 23.3509277 0.0050839799 3.253064e-01 4.687467e-01 8 123810352 123811455 1104 + 1.191 1.231 0.141
ENSG00000176853 E037 3.4546086 0.0673444666 9.055527e-01 9.439075e-01 8 123812056 123812177 122 + 0.480 0.605 0.586
ENSG00000176853 E038 671.3479157 0.0079945534 2.273273e-16 9.366350e-15 8 123812519 123815193 2675 + 2.553 2.993 1.463
ENSG00000176853 E039 70.1174181 0.0265581104 3.963086e-27 5.174327e-25 8 123815194 123815452 259 + 1.283 2.407 3.806