ENSG00000176731

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000612809 ENSG00000176731 HEK293_OSMI2_2hA HEK293_TMG_2hB RBIS protein_coding protein_coding 85.88469 69.51624 111.1561 5.951877 2.086045 0.6770871 25.970624 18.223930 40.272412 2.828371 1.3439542 1.1435245 0.2803333 0.2592333 0.36213333 0.10290000 0.0061397157 0.0001045127 FALSE TRUE
ENST00000614462 ENSG00000176731 HEK293_OSMI2_2hA HEK293_TMG_2hB RBIS protein_coding protein_coding 85.88469 69.51624 111.1561 5.951877 2.086045 0.6770871 9.484984 10.662775 7.378623 1.930746 0.5313170 -0.5305578 0.1146875 0.1511000 0.06663333 -0.08446667 0.0001045127 0.0001045127   FALSE
ENST00000619594 ENSG00000176731 HEK293_OSMI2_2hA HEK293_TMG_2hB RBIS protein_coding protein_coding 85.88469 69.51624 111.1561 5.951877 2.086045 0.6770871 31.677850 28.156798 43.441179 1.754164 0.3594835 0.6253997 0.3681167 0.4067333 0.39096667 -0.01576667 0.7766200994 0.0001045127 FALSE FALSE
MSTRG.31758.9 ENSG00000176731 HEK293_OSMI2_2hA HEK293_TMG_2hB RBIS protein_coding   85.88469 69.51624 111.1561 5.951877 2.086045 0.6770871 9.414764 7.254755 8.187173 1.660590 0.7109846 0.1742123 0.1183708 0.1074333 0.07353333 -0.03390000 0.6325071743 0.0001045127   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000176731 E001 2.805571 5.227457e-03 6.087513e-02 1.281102e-01 8 85214048 85214076 29 - 0.699 0.395 -1.432
ENSG00000176731 E002 13.069773 1.350118e-03 8.286732e-04 3.361735e-03 8 85214077 85214486 410 - 1.279 0.961 -1.144
ENSG00000176731 E003 2.147474 9.268442e-03 9.420771e-01 9.676307e-01 8 85214487 85214501 15 - 0.517 0.508 -0.043
ENSG00000176731 E004 7.522885 2.225810e-03 8.772550e-07 7.372608e-06 8 85214502 85214502 1 - 0.553 1.156 2.373
ENSG00000176731 E005 31.122387 4.905550e-02 6.482694e-01 7.598197e-01 8 85214503 85214525 23 - 1.533 1.486 -0.161
ENSG00000176731 E006 215.467748 2.147832e-04 1.325148e-01 2.375749e-01 8 85214526 85214555 30 - 2.322 2.360 0.126
ENSG00000176731 E007 277.533985 3.184655e-04 1.323220e-01 2.373346e-01 8 85214556 85214585 30 - 2.433 2.468 0.115
ENSG00000176731 E008 512.945603 1.657123e-04 1.958851e-03 7.097335e-03 8 85214586 85214631 46 - 2.690 2.740 0.164
ENSG00000176731 E009 531.162793 1.429201e-04 3.924846e-02 8.963798e-02 8 85214921 85215014 94 - 2.743 2.713 -0.100
ENSG00000176731 E010 450.296953 1.366739e-04 3.418233e-02 8.011060e-02 8 85215015 85215037 23 - 2.673 2.640 -0.112
ENSG00000176731 E011 7.943461 2.125464e-03 8.589093e-01 9.129704e-01 8 85215038 85215134 97 - 0.940 0.962 0.079
ENSG00000176731 E012 11.826017 3.576299e-02 8.452356e-04 3.420794e-03 8 85216813 85217159 347 - 1.272 0.795 -1.756
ENSG00000176731 E013 4.474881 4.000528e-03 2.997637e-03 1.025726e-02 8 85217160 85217292 133 - 0.895 0.455 -1.888
ENSG00000176731 E014 6.268740 6.839927e-02 4.064143e-03 1.332168e-02 8 85217293 85217385 93 - 1.042 0.508 -2.174
ENSG00000176731 E015 967.323245 9.515409e-05 1.387325e-01 2.463511e-01 8 85217386 85217502 117 - 2.981 2.998 0.055
ENSG00000176731 E016 21.500944 9.285739e-04 1.995472e-01 3.256646e-01 8 85217503 85217702 200 - 1.393 1.299 -0.328
ENSG00000176731 E017 6.776697 2.031201e-02 2.554264e-01 3.923123e-01 8 85218626 85218670 45 - 0.954 0.793 -0.618
ENSG00000176731 E018 5.301803 1.888995e-02 6.836220e-01 7.867757e-01 8 85218671 85218706 36 - 0.827 0.765 -0.245
ENSG00000176731 E019 9.228041 1.799730e-03 8.238475e-01 8.890148e-01 8 85218707 85218824 118 - 1.018 0.995 -0.085
ENSG00000176731 E020 23.999232 1.570300e-03 4.718923e-02 1.041711e-01 8 85218825 85219235 411 - 1.458 1.315 -0.497
ENSG00000176731 E021 12.692534 1.790483e-02 7.509930e-04 3.082713e-03 8 85219236 85219322 87 - 1.285 0.885 -1.454
ENSG00000176731 E022 67.308095 4.704172e-04 1.731573e-06 1.365644e-05 8 85219323 85219360 38 - 1.927 1.717 -0.707
ENSG00000176731 E023 45.955161 4.913806e-04 3.131803e-05 1.854965e-04 8 85219361 85219363 3 - 1.765 1.545 -0.746
ENSG00000176731 E024 620.939248 1.527204e-04 8.612487e-02 1.691945e-01 8 85220306 85220370 65 - 2.784 2.808 0.082
ENSG00000176731 E025 132.514464 1.130724e-02 1.488020e-01 2.600462e-01 8 85220371 85220421 51 - 2.087 2.161 0.248
ENSG00000176731 E026 3.176982 5.133531e-01 9.297065e-01 9.597220e-01 8 85220491 85220570 80 - 0.646 0.611 -0.155