ENSG00000176658

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000318217 ENSG00000176658 HEK293_OSMI2_2hA HEK293_TMG_2hB MYO1D protein_coding protein_coding 11.88173 12.23811 11.87447 0.1632908 0.2039581 -0.04348245 10.4673987 9.93775430 10.9132917 0.99998716 0.03877584 0.1349649 0.89071667 0.8141000 0.91966667 0.10556667 0.8034554292 0.0001293097 FALSE  
ENST00000394649 ENSG00000176658 HEK293_OSMI2_2hA HEK293_TMG_2hB MYO1D protein_coding protein_coding 11.88173 12.23811 11.87447 0.1632908 0.2039581 -0.04348245 0.3485381 1.43820028 0.0000000 0.76338921 0.00000000 -7.1781173 0.02456667 0.1169333 0.00000000 -0.11693333 0.1565482075 0.0001293097 FALSE  
ENST00000577352 ENSG00000176658 HEK293_OSMI2_2hA HEK293_TMG_2hB MYO1D protein_coding retained_intron 11.88173 12.23811 11.87447 0.1632908 0.2039581 -0.04348245 0.5743601 0.75711083 0.0000000 0.75711083 0.00000000 -6.2613631 0.04322083 0.0603000 0.00000000 -0.06030000 0.8260943563 0.0001293097 FALSE  
ENST00000579584 ENSG00000176658 HEK293_OSMI2_2hA HEK293_TMG_2hB MYO1D protein_coding protein_coding 11.88173 12.23811 11.87447 0.1632908 0.2039581 -0.04348245 0.3683883 0.06147917 0.7711242 0.04193438 0.16019753 3.4499571 0.03000417 0.0051000 0.06453333 0.05943333 0.0001293097 0.0001293097 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000176658 E001 0.4448795 0.4250632056 2.343831e-01 3.676211e-01 17 32492522 32492608 87 - 0.000 0.290 10.955
ENSG00000176658 E002 725.7673249 0.0064336490 2.872123e-10 4.689030e-09 17 32492609 32494452 1844 - 2.702 2.964 0.871
ENSG00000176658 E003 130.9445400 0.0002954038 4.839859e-06 3.458030e-05 17 32494453 32494718 266 - 2.009 2.201 0.643
ENSG00000176658 E004 113.7062898 0.0012162405 9.363899e-04 3.741353e-03 17 32494719 32494915 197 - 1.959 2.128 0.567
ENSG00000176658 E005 0.1515154 0.0424475024 4.637749e-01   17 32496956 32497073 118 - 0.120 0.000 -13.456
ENSG00000176658 E006 2.0136505 0.3038306194 4.664100e-01 6.059449e-01 17 32594203 32594340 138 - 0.548 0.381 -0.850
ENSG00000176658 E007 3.6377950 0.0054961745 1.467051e-02 3.974892e-02 17 32594341 32594566 226 - 0.806 0.477 -1.434
ENSG00000176658 E008 0.5546650 0.0212860684 6.031441e-01 7.239908e-01 17 32602552 32602711 160 - 0.120 0.222 1.070
ENSG00000176658 E009 131.5091615 0.0032758416 4.069708e-01 5.506038e-01 17 32605087 32605241 155 - 2.075 2.157 0.273
ENSG00000176658 E010 90.1236847 0.0003436540 9.678074e-01 9.838927e-01 17 32638722 32638774 53 - 1.934 1.980 0.156
ENSG00000176658 E011 86.6483513 0.0003417078 2.272854e-01 3.591520e-01 17 32638775 32638835 61 - 1.942 1.944 0.008
ENSG00000176658 E012 69.1595279 0.0072564159 3.535766e-01 4.978300e-01 17 32653843 32653863 21 - 1.851 1.846 -0.016
ENSG00000176658 E013 98.7983630 0.0004052201 5.203900e-01 6.543543e-01 17 32653864 32653947 84 - 1.983 2.007 0.082
ENSG00000176658 E014 114.5876054 0.0019095688 8.793850e-02 1.719887e-01 17 32654477 32654621 145 - 1.995 2.108 0.377
ENSG00000176658 E015 0.1817044 0.0392387098 5.127344e-01   17 32658522 32658978 457 - 0.000 0.125 12.877
ENSG00000176658 E016 0.0000000       17 32658979 32659114 136 -      
ENSG00000176658 E017 142.5304115 0.0026954319 1.040541e-01 1.966828e-01 17 32659115 32659338 224 - 2.094 2.202 0.362
ENSG00000176658 E018 0.0000000       17 32659339 32659406 68 -      
ENSG00000176658 E019 0.0000000       17 32659425 32659451 27 -      
ENSG00000176658 E020 0.2998086 0.0288136999 9.618573e-01   17 32684175 32684284 110 - 0.120 0.125 0.064
ENSG00000176658 E021 137.1224460 0.0002421607 1.816741e-01 3.033332e-01 17 32711988 32712195 208 - 2.135 2.143 0.026
ENSG00000176658 E022 82.5299676 0.0003198414 9.185157e-02 1.780099e-01 17 32721023 32721105 83 - 1.931 1.913 -0.058
ENSG00000176658 E023 60.6919790 0.0004784152 3.361346e-01 4.799531e-01 17 32721106 32721144 39 - 1.786 1.790 0.013
ENSG00000176658 E024 57.5332725 0.0119935631 9.426541e-02 1.817879e-01 17 32721145 32721189 45 - 1.801 1.733 -0.231
ENSG00000176658 E025 0.0000000       17 32727587 32727619 33 -      
ENSG00000176658 E026 0.2903454 0.2851637166 2.517566e-01   17 32734725 32734905 181 - 0.213 0.000 -13.518
ENSG00000176658 E027 71.3141951 0.0068357919 5.012143e-01 6.373705e-01 17 32738253 32738357 105 - 1.853 1.861 0.027
ENSG00000176658 E028 42.8076465 0.0026085592 4.565718e-01 5.969812e-01 17 32738358 32738385 28 - 1.639 1.643 0.013
ENSG00000176658 E029 48.6400102 0.0162401398 9.248301e-01 9.566561e-01 17 32745211 32745285 75 - 1.675 1.709 0.115
ENSG00000176658 E030 0.0000000       17 32745522 32745689 168 -      
ENSG00000176658 E031 67.6323077 0.0039019323 6.947574e-01 7.955064e-01 17 32748936 32749006 71 - 1.797 1.862 0.221
ENSG00000176658 E032 104.8204651 0.0005114357 1.054326e-01 1.987555e-01 17 32755492 32755662 171 - 2.028 2.018 -0.031
ENSG00000176658 E033 0.2998086 0.0288136999 9.618573e-01   17 32755663 32755688 26 - 0.120 0.125 0.064
ENSG00000176658 E034 0.1515154 0.0424475024 4.637749e-01   17 32755689 32755719 31 - 0.120 0.000 -13.456
ENSG00000176658 E035 0.0000000       17 32756207 32756262 56 -      
ENSG00000176658 E036 0.0000000       17 32759812 32760126 315 -      
ENSG00000176658 E037 77.2772873 0.0004203385 5.852120e-03 1.822356e-02 17 32760290 32760404 115 - 1.924 1.861 -0.212
ENSG00000176658 E038 86.2761045 0.0008271155 6.475789e-07 5.601145e-06 17 32760482 32760627 146 - 2.014 1.867 -0.496
ENSG00000176658 E039 98.7241650 0.0003366860 2.443840e-07 2.303031e-06 17 32764878 32765081 204 - 2.067 1.932 -0.452
ENSG00000176658 E040 71.2111308 0.0006818458 3.177938e-03 1.079099e-02 17 32767636 32767752 117 - 1.899 1.821 -0.261
ENSG00000176658 E041 76.9597896 0.0005962295 5.686104e-06 3.995606e-05 17 32771125 32771220 96 - 1.959 1.821 -0.462
ENSG00000176658 E042 64.5008223 0.0081765180 8.327342e-04 3.375831e-03 17 32772789 32772842 54 - 1.892 1.735 -0.529
ENSG00000176658 E043 93.5713752 0.0054187928 1.823100e-03 6.673509e-03 17 32775864 32776029 166 - 2.029 1.920 -0.366
ENSG00000176658 E044 71.3378262 0.0036704831 3.515758e-06 2.592138e-05 17 32778480 32778573 94 - 1.948 1.763 -0.624
ENSG00000176658 E045 91.1774105 0.0003578145 1.130382e-17 5.506491e-16 17 32780576 32780784 209 - 2.090 1.819 -0.911
ENSG00000176658 E046 0.1451727 0.0429209612 4.644883e-01   17 32792464 32792591 128 - 0.120 0.000 -13.453
ENSG00000176658 E047 0.0000000       17 32794193 32794299 107 -      
ENSG00000176658 E048 0.0000000       17 32862904 32863043 140 -      
ENSG00000176658 E049 46.5771455 0.0005039933 2.478544e-14 7.690023e-13 17 32876778 32877177 400 - 1.829 1.477 -1.197