ENSG00000176597

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000326505 ENSG00000176597 HEK293_OSMI2_2hA HEK293_TMG_2hB B3GNT5 protein_coding protein_coding 3.384271 0.8028974 5.738083 0.03621862 0.1595431 2.821936 2.1408175 0.6481654 3.71219039 0.02793121 0.56576071 2.499630 0.6472333 0.8079667 0.6429 -0.1650667 3.814734e-01 1.772882e-05 FALSE TRUE
ENST00000460419 ENSG00000176597 HEK293_OSMI2_2hA HEK293_TMG_2hB B3GNT5 protein_coding protein_coding 3.384271 0.8028974 5.738083 0.03621862 0.1595431 2.821936 0.8539969 0.0000000 1.69815756 0.00000000 0.34261553 7.416297 0.1519417 0.0000000 0.2996 0.2996000 1.109924e-04 1.772882e-05 FALSE TRUE
ENST00000480551 ENSG00000176597 HEK293_OSMI2_2hA HEK293_TMG_2hB B3GNT5 protein_coding processed_transcript 3.384271 0.8028974 5.738083 0.03621862 0.1595431 2.821936 0.1007263 0.1437430 0.09144523 0.01057808 0.03049819 -0.599820 0.1063042 0.1792333 0.0157 -0.1635333 1.772882e-05 1.772882e-05 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000176597 E001 1.7778249 0.008133366 1.329354e-01 2.381960e-01 3 183253253 183253282 30 + 0.393 0.000 -10.447
ENSG00000176597 E002 3.3999817 0.004497936 2.048758e-02 5.251969e-02 3 183253283 183253352 70 + 0.582 0.000 -12.310
ENSG00000176597 E003 6.4863329 0.003870546 7.577714e-01 8.424737e-01 3 183253353 183253472 120 + 0.753 0.726 -0.109
ENSG00000176597 E004 0.0000000       3 183253473 183253554 82 +      
ENSG00000176597 E005 0.0000000       3 183253555 183253562 8 +      
ENSG00000176597 E006 0.2924217 0.029078516 1.000000e+00   3 183253563 183253675 113 + 0.095 0.000 -9.265
ENSG00000176597 E007 7.6917734 0.061986285 1.581595e-01 2.726621e-01 3 183253795 183254233 439 + 0.838 0.557 -1.175
ENSG00000176597 E008 0.1472490 0.042872016 8.365268e-01   3 183254625 183254763 139 + 0.050 0.000 -8.278
ENSG00000176597 E009 0.0000000       3 183265246 183265301 56 +      
ENSG00000176597 E010 0.0000000       3 183265302 183265310 9 +      
ENSG00000176597 E011 0.0000000       3 183265311 183265320 10 +      
ENSG00000176597 E012 0.0000000       3 183265321 183265343 23 +      
ENSG00000176597 E013 0.0000000       3 183265344 183265506 163 +      
ENSG00000176597 E014 0.0000000       3 183265507 183265523 17 +      
ENSG00000176597 E015 0.0000000       3 183265524 183265574 51 +      
ENSG00000176597 E016 0.1472490 0.042872016 8.365268e-01   3 183265966 183266124 159 + 0.050 0.000 -8.278
ENSG00000176597 E017 65.0645801 0.001116166 4.130916e-13 1.076612e-11 3 183269498 183270253 756 + 1.721 1.171 -1.900
ENSG00000176597 E018 234.1273460 0.007178922 3.741315e-02 8.624632e-02 3 183270254 183273385 3132 + 2.211 2.282 0.238
ENSG00000176597 E019 0.0000000       3 183287480 183287604 125 +      
ENSG00000176597 E020 3.8278116 0.005521353 3.678045e-09 4.907696e-08 3 183287605 183287868 264 + 0.269 1.145 3.918
ENSG00000176597 E021 7.2053869 0.006234459 2.405948e-13 6.482713e-12 3 183296146 183298504 2359 + 0.489 1.386 3.483