ENSG00000176454

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000314891 ENSG00000176454 HEK293_OSMI2_2hA HEK293_TMG_2hB LPCAT4 protein_coding protein_coding 21.52696 18.95756 18.09478 1.153943 0.8247746 -0.06716381 3.250011 4.7836000 0.6541258 0.2055846 0.06197402 -2.85158111 0.15627917 0.25496667 0.03600000 -0.21896667 1.320850e-19 1.32085e-19 FALSE TRUE
ENST00000562404 ENSG00000176454 HEK293_OSMI2_2hA HEK293_TMG_2hB LPCAT4 protein_coding retained_intron 21.52696 18.95756 18.09478 1.153943 0.8247746 -0.06716381 2.012897 0.9916999 2.6298574 0.2429072 0.48488369 1.39800971 0.09351250 0.05123333 0.14643333 0.09520000 1.162876e-02 1.32085e-19 FALSE FALSE
ENST00000563240 ENSG00000176454 HEK293_OSMI2_2hA HEK293_TMG_2hB LPCAT4 protein_coding retained_intron 21.52696 18.95756 18.09478 1.153943 0.8247746 -0.06716381 2.517578 1.3178476 2.4659022 0.0831348 0.27134984 0.89886470 0.11372083 0.06966667 0.13800000 0.06833333 2.901951e-02 1.32085e-19 FALSE TRUE
ENST00000563748 ENSG00000176454 HEK293_OSMI2_2hA HEK293_TMG_2hB LPCAT4 protein_coding retained_intron 21.52696 18.95756 18.09478 1.153943 0.8247746 -0.06716381 4.047484 3.2922622 3.4431292 0.1982785 0.42199092 0.06444964 0.18566667 0.17373333 0.18966667 0.01593333 8.354253e-01 1.32085e-19 TRUE TRUE
ENST00000569804 ENSG00000176454 HEK293_OSMI2_2hA HEK293_TMG_2hB LPCAT4 protein_coding retained_intron 21.52696 18.95756 18.09478 1.153943 0.8247746 -0.06716381 1.222246 0.0000000 1.5100451 0.0000000 0.13716522 7.24797036 0.05416667 0.00000000 0.08423333 0.08423333 1.566606e-18 1.32085e-19   FALSE
MSTRG.10446.3 ENSG00000176454 HEK293_OSMI2_2hA HEK293_TMG_2hB LPCAT4 protein_coding   21.52696 18.95756 18.09478 1.153943 0.8247746 -0.06716381 5.473044 6.6787962 4.1730198 0.8375687 0.09840070 -0.67720175 0.25883333 0.35016667 0.23156667 -0.11860000 1.305163e-02 1.32085e-19 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000176454 E001 0.8783198 0.0137038138 4.253225e-01 5.682655e-01 15 34358633 34358908 276 - 0.154 0.299 1.216
ENSG00000176454 E002 19.3515532 0.0011750771 6.893821e-01 7.912884e-01 15 34358909 34359025 117 - 1.257 1.292 0.122
ENSG00000176454 E003 64.6990858 0.0024034042 8.216205e-03 2.433182e-02 15 34359026 34359095 70 - 1.693 1.833 0.474
ENSG00000176454 E004 144.8265046 0.0048571463 4.851534e-03 1.551814e-02 15 34359096 34359302 207 - 2.045 2.174 0.432
ENSG00000176454 E005 113.7815599 0.0067488951 2.006290e-02 5.161752e-02 15 34359589 34359745 157 - 1.943 2.069 0.421
ENSG00000176454 E006 41.1602632 0.0107396733 1.920296e-05 1.196180e-04 15 34359746 34360110 365 - 1.775 1.466 -1.053
ENSG00000176454 E007 96.4581701 0.0024644470 1.974538e-01 3.230369e-01 15 34360111 34360209 99 - 1.919 1.979 0.202
ENSG00000176454 E008 10.2319185 0.0016478292 3.474000e-06 2.564744e-05 15 34360754 34360827 74 - 1.277 0.810 -1.718
ENSG00000176454 E009 16.2288620 0.0091011560 8.854110e-11 1.576934e-09 15 34360912 34361168 257 - 1.519 0.913 -2.158
ENSG00000176454 E010 12.4892317 0.0156838131 4.951010e-06 3.526674e-05 15 34361169 34361229 61 - 1.374 0.884 -1.766
ENSG00000176454 E011 14.8789408 0.0012243243 3.525367e-11 6.707736e-10 15 34361230 34361399 170 - 1.472 0.900 -2.044
ENSG00000176454 E012 93.7610864 0.0014660859 3.569496e-02 8.299240e-02 15 34361400 34361532 133 - 1.884 1.974 0.303
ENSG00000176454 E013 129.2826892 0.0003247946 3.014949e-01 4.433089e-01 15 34362196 34362321 126 - 2.059 2.095 0.120
ENSG00000176454 E014 64.4696076 0.0004658004 9.099815e-01 9.467683e-01 15 34362573 34362578 6 - 1.778 1.786 0.026
ENSG00000176454 E015 94.4190180 0.0003338435 5.062533e-01 6.419041e-01 15 34362579 34362655 77 - 1.930 1.958 0.093
ENSG00000176454 E016 5.3423730 0.0036926007 8.587775e-02 1.687860e-01 15 34362656 34362781 126 - 0.917 0.696 -0.873
ENSG00000176454 E017 73.8533368 0.0006802137 6.830665e-01 7.863834e-01 15 34362782 34362836 55 - 1.830 1.851 0.069
ENSG00000176454 E018 7.2950409 0.0728894886 4.756473e-01 6.143511e-01 15 34362837 34362890 54 - 0.981 0.842 -0.526
ENSG00000176454 E019 69.2954192 0.0004238779 7.325197e-01 8.239640e-01 15 34363422 34363456 35 - 1.802 1.819 0.059
ENSG00000176454 E020 15.8246201 0.0110837543 4.873939e-07 4.318770e-06 15 34363457 34363660 204 - 1.459 0.976 -1.715
ENSG00000176454 E021 84.6139557 0.0003277888 7.590303e-01 8.434872e-01 15 34363661 34363719 59 - 1.889 1.904 0.049
ENSG00000176454 E022 8.8918901 0.0038151288 4.741703e-05 2.686870e-04 15 34363720 34363740 21 - 1.203 0.757 -1.663
ENSG00000176454 E023 5.8197490 0.0089827690 7.178768e-03 2.168845e-02 15 34363946 34364012 67 - 1.017 0.673 -1.340
ENSG00000176454 E024 67.7384248 0.0025657117 3.743020e-02 8.627723e-02 15 34364013 34364073 61 - 1.725 1.834 0.368
ENSG00000176454 E025 3.8332290 0.0042396774 3.311255e-03 1.118016e-02 15 34364074 34364193 120 - 0.894 0.475 -1.785
ENSG00000176454 E026 110.5652665 0.0041938806 1.027044e-01 1.946248e-01 15 34364194 34364306 113 - 1.960 2.038 0.263
ENSG00000176454 E027 2.2142959 0.0067087939 1.239749e-01 2.256360e-01 15 34364607 34365007 401 - 0.645 0.396 -1.201
ENSG00000176454 E028 104.8353581 0.0013815675 7.486481e-01 8.358131e-01 15 34365008 34365098 91 - 1.997 1.987 -0.033
ENSG00000176454 E029 103.5124604 0.0006191410 8.320185e-01 8.947158e-01 15 34365099 34365228 130 - 1.989 1.984 -0.018
ENSG00000176454 E030 3.1773840 0.0113920491 2.390282e-02 5.960508e-02 15 34365229 34365558 330 - 0.787 0.438 -1.559
ENSG00000176454 E031 57.5530102 0.0004287596 1.395191e-01 2.473675e-01 15 34365559 34365701 143 - 1.681 1.756 0.255
ENSG00000176454 E032 8.3681166 0.0102395866 1.098200e-12 2.674861e-11 15 34365702 34365994 293 - 1.324 0.438 -3.529
ENSG00000176454 E033 3.9608706 0.0122508954 4.416292e-03 1.430886e-02 15 34366914 34366986 73 - 0.894 0.476 -1.781
ENSG00000176454 E034 21.2609562 0.0131536109 1.037002e-01 1.961353e-01 15 34366987 34367196 210 - 1.191 1.355 0.574