ENSG00000176444

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000355560 ENSG00000176444 HEK293_OSMI2_2hA HEK293_TMG_2hB CLK2 protein_coding protein_coding 69.18289 59.71622 70.86549 1.517305 3.790623 0.2469224 5.379656 6.8595874 2.479065 0.9987623 1.8696544 -1.4646196 0.07997083 0.11536667 0.03660000 -0.07876667 4.219208e-01 1.406899e-16 FALSE TRUE
ENST00000361168 ENSG00000176444 HEK293_OSMI2_2hA HEK293_TMG_2hB CLK2 protein_coding protein_coding 69.18289 59.71622 70.86549 1.517305 3.790623 0.2469224 5.733676 6.0880542 8.859507 1.2908325 2.0791098 0.5405050 0.08292083 0.10143333 0.12406667 0.02263333 8.372787e-01 1.406899e-16 FALSE TRUE
ENST00000368361 ENSG00000176444 HEK293_OSMI2_2hA HEK293_TMG_2hB CLK2 protein_coding protein_coding 69.18289 59.71622 70.86549 1.517305 3.790623 0.2469224 22.472084 27.6690309 18.906376 1.6150284 0.4243795 -0.5491577 0.33488333 0.46333333 0.26880000 -0.19453333 6.136329e-05 1.406899e-16 FALSE TRUE
ENST00000484699 ENSG00000176444 HEK293_OSMI2_2hA HEK293_TMG_2hB CLK2 protein_coding processed_transcript 69.18289 59.71622 70.86549 1.517305 3.790623 0.2469224 11.310881 2.8148252 14.574072 0.4268838 2.1379033 2.3681601 0.15635000 0.04706667 0.20373333 0.15666667 3.922683e-10 1.406899e-16 FALSE TRUE
ENST00000497188 ENSG00000176444 HEK293_OSMI2_2hA HEK293_TMG_2hB CLK2 protein_coding processed_transcript 69.18289 59.71622 70.86549 1.517305 3.790623 0.2469224 11.425623 4.5858138 13.002185 0.8058410 0.7876366 1.5014710 0.15926250 0.07623333 0.18336667 0.10713333 5.228063e-06 1.406899e-16 TRUE TRUE
MSTRG.2309.1 ENSG00000176444 HEK293_OSMI2_2hA HEK293_TMG_2hB CLK2 protein_coding   69.18289 59.71622 70.86549 1.517305 3.790623 0.2469224 2.582264 0.9344025 5.358503 0.1980303 1.0032071 2.5070461 0.03700417 0.01566667 0.07666667 0.06100000 2.235138e-04 1.406899e-16 FALSE TRUE
MSTRG.2309.7 ENSG00000176444 HEK293_OSMI2_2hA HEK293_TMG_2hB CLK2 protein_coding   69.18289 59.71622 70.86549 1.517305 3.790623 0.2469224 1.943600 5.1138027 0.000000 1.3516711 0.0000000 -9.0010711 0.03132917 0.08680000 0.00000000 -0.08680000 1.406899e-16 1.406899e-16 FALSE TRUE
MSTRG.2309.8 ENSG00000176444 HEK293_OSMI2_2hA HEK293_TMG_2hB CLK2 protein_coding   69.18289 59.71622 70.86549 1.517305 3.790623 0.2469224 5.521127 2.9614085 5.440367 2.3155847 2.7820803 0.8752064 0.07722500 0.04790000 0.07503333 0.02713333 9.096208e-01 1.406899e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000176444 E001 11.98831 0.0070538146 3.565271e-08 3.954044e-07 1 155262734 155262867 134 - 1.349 0.755 -2.185
ENSG00000176444 E002 16.99571 0.0497658026 8.012147e-01 8.733335e-01 1 155262868 155262873 6 - 1.238 1.272 0.121
ENSG00000176444 E003 20.16118 0.0456727549 2.097413e-01 3.382438e-01 1 155262874 155262879 6 - 1.229 1.390 0.561
ENSG00000176444 E004 32.71850 0.0122343304 5.058776e-01 6.415909e-01 1 155262880 155262885 6 - 1.498 1.552 0.186
ENSG00000176444 E005 87.05264 0.0260778724 1.197306e-01 2.195885e-01 1 155262886 155262922 37 - 1.859 1.998 0.470
ENSG00000176444 E006 921.82220 0.0032375668 1.691371e-10 2.871450e-09 1 155262923 155263400 478 - 2.852 3.030 0.589
ENSG00000176444 E007 548.89669 0.0011808980 6.530355e-09 8.336070e-08 1 155263950 155264040 91 - 2.660 2.786 0.419
ENSG00000176444 E008 52.08544 0.0004201602 1.204184e-07 1.204801e-06 1 155264041 155264220 180 - 1.848 1.586 -0.886
ENSG00000176444 E009 536.32547 0.0013411394 1.060886e-03 4.169569e-03 1 155264221 155264300 80 - 2.682 2.755 0.245
ENSG00000176444 E010 51.71160 0.0004543666 1.249512e-09 1.817260e-08 1 155264301 155264467 167 - 1.860 1.557 -1.027
ENSG00000176444 E011 466.87743 0.0001413484 2.050389e-04 9.856133e-04 1 155264468 155264550 83 - 2.630 2.690 0.200
ENSG00000176444 E012 568.01471 0.0001126181 5.004320e-03 1.594171e-02 1 155264645 155264774 130 - 2.726 2.766 0.133
ENSG00000176444 E013 448.61263 0.0001610310 1.732876e-02 4.566787e-02 1 155265860 155265954 95 - 2.625 2.664 0.129
ENSG00000176444 E014 556.24907 0.0001926776 2.661465e-03 9.256932e-03 1 155266729 155266895 167 - 2.714 2.759 0.150
ENSG00000176444 E015 459.82890 0.0001341514 1.968444e-01 3.222938e-01 1 155268010 155268093 84 - 2.646 2.665 0.064
ENSG00000176444 E016 298.48399 0.0001541068 5.098836e-01 6.451641e-01 1 155268094 155268126 33 - 2.464 2.475 0.037
ENSG00000176444 E017 358.20326 0.0017909164 8.389079e-01 8.993242e-01 1 155268293 155268359 67 - 2.554 2.546 -0.025
ENSG00000176444 E018 175.22238 0.0050686334 5.139673e-27 6.637325e-25 1 155268360 155268707 348 - 2.448 1.963 -1.621
ENSG00000176444 E019 300.23626 0.0009891814 2.620007e-01 3.997202e-01 1 155268708 155268792 85 - 2.458 2.483 0.085
ENSG00000176444 E020 108.97165 0.0004055915 2.856866e-01 4.260404e-01 1 155268793 155268795 3 - 2.015 2.050 0.115
ENSG00000176444 E021 121.04547 0.0180525912 5.088874e-13 1.308813e-11 1 155268796 155269165 370 - 2.303 1.766 -1.803
ENSG00000176444 E022 157.51804 0.0086360888 1.314077e-22 1.121892e-20 1 155269166 155269487 322 - 2.418 1.877 -1.813
ENSG00000176444 E023 534.19939 0.0019566162 9.070885e-04 3.638699e-03 1 155269488 155269713 226 - 2.771 2.684 -0.290
ENSG00000176444 E024 123.22596 0.0031126209 6.070028e-05 3.348910e-04 1 155269714 155269716 3 - 2.178 2.008 -0.569
ENSG00000176444 E025 463.32744 0.0026567134 1.317037e-04 6.663692e-04 1 155270808 155270977 170 - 2.723 2.610 -0.377
ENSG00000176444 E026 273.73615 0.0010757928 1.328868e-04 6.716199e-04 1 155273201 155273504 304 - 2.488 2.385 -0.343
ENSG00000176444 E027 0.00000       1 155278375 155278491 117 -