ENSG00000176387

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000326152 ENSG00000176387 HEK293_OSMI2_2hA HEK293_TMG_2hB HSD11B2 protein_coding protein_coding 1.477148 2.975141 0.4705098 0.4402768 0.07292756 -2.635161 0.8860361 1.7872683 0.40493047 0.1919369 0.05446861 -2.114864 0.61922917 0.6094000 0.87096667 0.26156667 0.043683433 0.000915399 FALSE TRUE
ENST00000566606 ENSG00000176387 HEK293_OSMI2_2hA HEK293_TMG_2hB HSD11B2 protein_coding nonsense_mediated_decay 1.477148 2.975141 0.4705098 0.4402768 0.07292756 -2.635161 0.1005790 0.2611790 0.03296551 0.1684890 0.03296551 -2.657995 0.06377083 0.0866000 0.07560000 -0.01100000 0.950003237 0.000915399 FALSE FALSE
ENST00000567684 ENSG00000176387 HEK293_OSMI2_2hA HEK293_TMG_2hB HSD11B2 protein_coding processed_transcript 1.477148 2.975141 0.4705098 0.4402768 0.07292756 -2.635161 0.3259917 0.8039093 0.00000000 0.2180602 0.00000000 -6.346796 0.18249583 0.2706667 0.00000000 -0.27066667 0.000915399 0.000915399 FALSE FALSE
ENST00000569303 ENSG00000176387 HEK293_OSMI2_2hA HEK293_TMG_2hB HSD11B2 protein_coding retained_intron 1.477148 2.975141 0.4705098 0.4402768 0.07292756 -2.635161 0.1645415 0.1227843 0.03261378 0.1227843 0.03261378 -1.639693 0.13451250 0.0333000 0.05343333 0.02013333 0.803809582 0.000915399   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000176387 E001 3.5925837 0.0073647313 0.02741737 0.06683916 16 67430652 67430779 128 + 0.001 0.561 10.825
ENSG00000176387 E002 0.0000000       16 67431121 67431121 1 +      
ENSG00000176387 E003 0.0000000       16 67431122 67431148 27 +      
ENSG00000176387 E004 0.1515154 0.0483415581 0.05679083   16 67431149 67431365 217 + 0.256 0.000 -12.949
ENSG00000176387 E005 1.1427281 0.0581980336 0.23820126 0.37205470 16 67431366 67431513 148 + 0.416 0.186 -1.589
ENSG00000176387 E006 1.0934354 0.1737356942 0.82636340 0.89073649 16 67432634 67433184 551 + 0.256 0.223 -0.260
ENSG00000176387 E007 2.4251280 0.0090446153 0.87242479 0.92195508 16 67435533 67435627 95 + 0.416 0.390 -0.144
ENSG00000176387 E008 15.0979337 0.0013583743 0.21089957 0.33960600 16 67435628 67435840 213 + 0.871 1.037 0.624
ENSG00000176387 E009 14.4649067 0.0015167634 0.26259895 0.40037878 16 67435957 67436142 186 + 0.871 1.021 0.565
ENSG00000176387 E010 12.4639206 0.0015584993 0.52014982 0.65416082 16 67436249 67436386 138 + 0.871 0.963 0.348
ENSG00000176387 E011 47.5293495 0.0007771626 0.03244802 0.07671414 16 67436588 67437553 966 + 1.579 1.483 -0.328