ENSG00000176087

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000323146 ENSG00000176087 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC35A4 protein_coding protein_coding 95.22488 156.3829 63.00827 7.240932 0.8198682 -1.311333 79.43400 135.22215 46.82260 4.867011 0.8814653 -1.5298532 0.8203417 0.8655333 0.7430333 -0.1225000 2.390924e-08 1.214372e-09 FALSE TRUE
ENST00000623481 ENSG00000176087 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC35A4 protein_coding protein_coding 95.22488 156.3829 63.00827 7.240932 0.8198682 -1.311333 14.22861 19.78068 14.46002 2.288465 0.1030363 -0.4517538 0.1606250 0.1256667 0.2295333 0.1038667 3.112413e-07 1.214372e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000176087 E001 179.27331 0.0011999420 1.064348e-12 2.599426e-11 5 140564446 140564818 373 + 2.284 2.174 -0.367
ENSG00000176087 E002 77.61714 0.0009678274 5.091665e-07 4.498021e-06 5 140564819 140564827 9 + 1.921 1.816 -0.355
ENSG00000176087 E003 390.06545 0.0050277310 4.078074e-03 1.336272e-02 5 140564828 140564858 31 + 2.538 2.551 0.042
ENSG00000176087 E004 25.68801 0.0012848279 1.129027e-16 4.820618e-15 5 140564891 140565747 857 + 1.651 1.192 -1.588
ENSG00000176087 E005 13.20305 0.0013214807 6.952917e-05 3.777377e-04 5 140565748 140565872 125 + 1.282 1.023 -0.928
ENSG00000176087 E006 725.16564 0.0038029010 2.167099e-03 7.741554e-03 5 140565873 140565971 99 + 2.797 2.824 0.091
ENSG00000176087 E007 7979.28870 0.0046252476 3.469625e-06 2.562413e-05 5 140566565 140569100 2536 + 3.747 3.897 0.497