ENSG00000175854

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000418976 ENSG00000175854 HEK293_OSMI2_2hA HEK293_TMG_2hB SWI5 protein_coding protein_coding 79.86375 131.6797 52.28691 8.088003 0.6236634 -1.332345 65.80058 107.15605 44.199648 8.1550122 1.2597672 -1.277415 0.8271292 0.8122333 0.8450 0.03276667 0.49267051 0.03884956 FALSE  
ENST00000608796 ENSG00000175854 HEK293_OSMI2_2hA HEK293_TMG_2hB SWI5 protein_coding protein_coding 79.86375 131.6797 52.28691 8.088003 0.6236634 -1.332345 10.60711 19.57484 5.069112 0.6489852 0.3168686 -1.947089 0.1262292 0.1501667 0.0971 -0.05306667 0.03884956 0.03884956 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000175854 E001 4.709178 0.0035351056 0.9952340780 1.000000000 9 128275379 128275438 60 + 0.691 0.697 0.027
ENSG00000175854 E002 5.525974 0.0030091380 0.7723355910 0.853040429 9 128275439 128275496 58 + 0.780 0.744 -0.143
ENSG00000175854 E003 2.394865 0.0067743147 0.7520965674 0.838383506 9 128276222 128276241 20 + 0.427 0.489 0.317
ENSG00000175854 E004 3.415148 0.0424493848 0.6360105154 0.750151785 9 128276242 128276255 14 + 0.510 0.605 0.441
ENSG00000175854 E005 22.761332 0.0187969658 0.8117997180 0.880583463 9 128276256 128276275 20 + 1.313 1.287 -0.090
ENSG00000175854 E006 53.908148 0.0118764995 0.9713806992 0.986122012 9 128276276 128276287 12 + 1.654 1.657 0.008
ENSG00000175854 E007 452.393690 0.0025651120 0.2010262778 0.327456323 9 128276288 128276402 115 + 2.545 2.584 0.131
ENSG00000175854 E008 124.867522 0.0053574217 0.0008604479 0.003474937 9 128276587 128276706 120 + 1.873 2.051 0.597
ENSG00000175854 E009 457.634187 0.0007549563 0.2563695674 0.393326317 9 128276707 128276755 49 + 2.597 2.577 -0.067
ENSG00000175854 E010 1.359820 0.0118377734 0.9965949647 1.000000000 9 128278634 128278681 48 + 0.326 0.330 0.028
ENSG00000175854 E011 741.022299 0.0001373408 0.6282187420 0.743796637 9 128284510 128284631 122 + 2.793 2.793 -0.002
ENSG00000175854 E012 3.584534 0.0088724783 0.8063649967 0.876917594 9 128285505 128285778 274 + 0.638 0.605 -0.146
ENSG00000175854 E013 699.397704 0.0011115523 0.0324690927 0.076753415 9 128285939 128286033 95 + 2.794 2.758 -0.119
ENSG00000175854 E014 16.493706 0.2509276627 0.5914346899 0.714216228 9 128286034 128286534 501 + 0.973 1.214 0.872
ENSG00000175854 E015 821.371974 0.0025544950 0.5492355869 0.678993717 9 128288652 128288990 339 + 2.821 2.838 0.057
ENSG00000175854 E016 5.976266 0.0027243690 0.7097376886 0.807030629 9 128313830 128313905 76 + 0.818 0.773 -0.180
ENSG00000175854 E017 2.025012 0.0070157323 0.1401172627 0.248177293 9 128314098 128314247 150 + 0.193 0.489 1.901
ENSG00000175854 E018 36.148364 0.0052353842 0.0269166217 0.065815757 9 128315424 128316123 700 + 1.603 1.447 -0.533