ENSG00000175643

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000312499 ENSG00000175643 HEK293_OSMI2_2hA HEK293_TMG_2hB RMI2 protein_coding protein_coding 31.09839 43.7021 21.80022 2.444774 0.5291542 -1.003028 16.12891 17.40152 15.315053 2.130537 0.3017338 -0.1841499 0.5412208 0.4009 0.7031667 0.3022667 0.0001575177 0.0001575177 FALSE TRUE
ENST00000576027 ENSG00000175643 HEK293_OSMI2_2hA HEK293_TMG_2hB RMI2 protein_coding protein_coding 31.09839 43.7021 21.80022 2.444774 0.5291542 -1.003028 14.75280 26.06121 6.356168 3.432648 0.5159389 -2.0339603 0.4516375 0.5934 0.2910333 -0.3023667 0.0001698650 0.0001575177   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000175643 E001 0.0000000       16 11249619 11249626 8 +      
ENSG00000175643 E002 0.0000000       16 11249627 11249648 22 +      
ENSG00000175643 E003 0.0000000       16 11249649 11249778 130 +      
ENSG00000175643 E004 0.0000000       16 11295216 11295294 79 +      
ENSG00000175643 E005 0.0000000       16 11312829 11312899 71 +      
ENSG00000175643 E006 0.0000000       16 11312900 11312937 38 +      
ENSG00000175643 E007 0.0000000       16 11316141 11316451 311 +      
ENSG00000175643 E008 0.0000000       16 11316452 11316884 433 +      
ENSG00000175643 E009 0.1482932 0.040962210 9.259894e-01   16 11316885 11317036 152 + 0.000 0.113 8.013
ENSG00000175643 E010 8.9130262 0.003997824 4.469523e-10 7.066159e-09 16 11345450 11345458 9 + 1.211 0.714 -1.871
ENSG00000175643 E011 17.1462430 0.022575510 1.574976e-09 2.251883e-08 16 11345459 11345469 11 + 1.458 1.012 -1.579
ENSG00000175643 E012 30.6822384 0.044834111 3.269820e-09 4.405189e-08 16 11345470 11345543 74 + 1.714 1.218 -1.710
ENSG00000175643 E013 109.8924277 0.006400435 1.520616e-07 1.491232e-06 16 11345544 11345766 223 + 2.061 2.021 -0.134
ENSG00000175643 E014 1040.8963229 0.014925287 5.710700e-12 1.236853e-10 16 11350642 11351760 1119 + 2.819 3.107 0.960
ENSG00000175643 E015 0.8396112 0.689535566 7.994992e-01 8.720956e-01 16 11379822 11379887 66 + 0.233 0.280 0.353
ENSG00000175643 E016 5.6273020 0.016856627 7.889273e-01 8.648900e-01 16 11380798 11381662 865 + 0.721 0.862 0.560