ENSG00000175395

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000302609 ENSG00000175395 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF25 protein_coding protein_coding 0.8014788 0.1534881 1.510005 0.01566989 0.1410474 3.216819 0.52951773 0.1534881 0.8776840 0.01566989 0.04893045 2.440861 0.79020417 1 0.5886333 -0.4113667 0.006249313 0.006249313 FALSE TRUE
ENST00000374633 ENSG00000175395 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF25 protein_coding processed_transcript 0.8014788 0.1534881 1.510005 0.01566989 0.1410474 3.216819 0.09605906 0.0000000 0.1955506 0.00000000 0.09976574 4.361422 0.08293333 0 0.1423667 0.1423667 0.721553858 0.006249313 FALSE TRUE
MSTRG.3795.3 ENSG00000175395 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF25 protein_coding   0.8014788 0.1534881 1.510005 0.01566989 0.1410474 3.216819 0.16736852 0.0000000 0.4367707 0.00000000 0.21509159 5.481463 0.12132500 0 0.2690000 0.2690000 0.183904074 0.006249313 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000175395 E001 12.524798 0.001574287 0.000305231 0.00140070 10 37949573 37950566 994 - 0.920 1.270 1.268
ENSG00000175395 E002 40.557323 0.010864776 0.488267975 0.62586455 10 37950567 37952974 2408 - 1.464 1.490 0.088
ENSG00000175395 E003 4.813490 0.004477711 0.297841014 0.43921710 10 37952975 37953062 88 - 0.663 0.442 -1.030
ENSG00000175395 E004 5.836960 0.002946929 0.033201403 0.07819948 10 37953063 37953195 133 - 0.745 0.275 -2.370
ENSG00000175395 E005 0.000000       10 37953283 37953364 82 -      
ENSG00000175395 E006 3.544122 0.004825174 0.194104331 0.31892948 10 37953695 37953758 64 - 0.575 0.275 -1.647
ENSG00000175395 E007 4.735027 0.003626439 0.076563164 0.15399258 10 37957020 37957115 96 - 0.674 0.275 -2.077
ENSG00000175395 E008 4.348335 0.004929952 0.390765707 0.53471652 10 37957420 37957546 127 - 0.627 0.442 -0.877
ENSG00000175395 E009 0.000000       10 37967917 37967939 23 -      
ENSG00000175395 E010 2.192125 0.459654335 0.294194371 0.43529616 10 37971708 37971807 100 - 0.445 0.000 -11.043
ENSG00000175395 E011 1.173953 0.012915416 0.368099660 0.51249633 10 37971915 37972021 107 - 0.292 0.000 -10.785
ENSG00000175395 E012 1.109319 0.011888959 0.830872231 0.89395244 10 37976506 37976647 142 - 0.236 0.275 0.293