ENSG00000175348

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000309134 ENSG00000175348 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM9B protein_coding protein_coding 14.40551 12.5867 18.7536 1.929502 0.9589557 0.5748906 4.107192 5.061762 4.339312 1.1735395 1.4019772 -0.2216999 0.3114125 0.3937000 0.2293667 -0.16433333 0.20686644 0.01303995 FALSE FALSE
ENST00000525069 ENSG00000175348 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM9B protein_coding protein_coding 14.40551 12.5867 18.7536 1.929502 0.9589557 0.5748906 3.695849 1.536840 6.062008 0.3628034 0.7943555 1.9728494 0.2314708 0.1231333 0.3261333 0.20300000 0.01303995 0.01303995 FALSE TRUE
ENST00000534025 ENSG00000175348 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM9B protein_coding protein_coding 14.40551 12.5867 18.7536 1.929502 0.9589557 0.5748906 6.326669 5.949747 8.026895 0.6423309 0.7929738 0.4313871 0.4367250 0.4795333 0.4269000 -0.05263333 0.65195450 0.01303995 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000175348 E001 0.5932745 0.0193256438 8.543656e-01 9.098933e-01 11 8947202 8947293 92 - 0.198 0.238 0.333
ENSG00000175348 E002 77.1965321 0.0039573962 1.990001e-06 1.548826e-05 11 8947294 8947500 207 - 1.764 2.008 0.823
ENSG00000175348 E003 489.9316321 0.0020304339 1.720923e-01 2.909237e-01 11 8947501 8948475 975 - 2.678 2.712 0.113
ENSG00000175348 E004 3.5911467 0.0045387576 2.123454e-02 5.408180e-02 11 8952692 8953202 511 - 0.793 0.452 -1.511
ENSG00000175348 E005 102.3793315 0.0003695299 1.890369e-05 1.179051e-04 11 8953203 8953261 59 - 2.076 1.946 -0.436
ENSG00000175348 E006 104.5218874 0.0004137203 6.432160e-03 1.974613e-02 11 8953262 8953337 76 - 2.064 1.989 -0.253
ENSG00000175348 E007 96.7351904 0.0005312015 6.994046e-01 7.991695e-01 11 8956190 8956298 109 - 1.994 1.995 0.005
ENSG00000175348 E008 57.0540196 0.0033254107 4.711119e-01 6.102531e-01 11 8962092 8962183 92 - 1.779 1.752 -0.091
ENSG00000175348 E009 0.4783925 0.0212774960 6.999916e-01 7.995810e-01 11 8962695 8962852 158 - 0.198 0.135 -0.663
ENSG00000175348 E010 0.2944980 0.4571580554 3.929491e-01   11 8963991 8964208 218 - 0.198 0.000 -9.494
ENSG00000175348 E011 66.6994950 0.0037728612 7.123343e-01 8.091134e-01 11 8964209 8964442 234 - 1.820 1.850 0.101
ENSG00000175348 E012 7.3018162 0.0023983624 1.271996e-01 2.302331e-01 11 8964890 8965011 122 - 0.999 0.836 -0.618