ENSG00000175265

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000359187 ENSG00000175265 HEK293_OSMI2_2hA HEK293_TMG_2hB GOLGA8A protein_coding protein_coding 11.02785 6.546034 10.39775 1.473182 0.2663785 0.6667622 0.1648537 0.7389706 0.0000000 0.1484765 0.0000000 -6.2268372 0.02610000 0.12123333 0.0000000 -0.12123333 3.099910e-10 9.212412e-17 FALSE TRUE
ENST00000432566 ENSG00000175265 HEK293_OSMI2_2hA HEK293_TMG_2hB GOLGA8A protein_coding protein_coding 11.02785 6.546034 10.39775 1.473182 0.2663785 0.6667622 4.2523267 3.0934516 2.7429338 0.7336541 0.4190015 -0.1729037 0.37330833 0.47313333 0.2620667 -0.21106667 2.356727e-02 9.212412e-17 FALSE TRUE
ENST00000473125 ENSG00000175265 HEK293_OSMI2_2hA HEK293_TMG_2hB GOLGA8A protein_coding retained_intron 11.02785 6.546034 10.39775 1.473182 0.2663785 0.6667622 4.5832041 2.1962685 4.3028302 0.6272858 0.6618584 0.9670266 0.41772917 0.32780000 0.4158667 0.08806667 6.376566e-01 9.212412e-17 FALSE TRUE
MSTRG.10452.12 ENSG00000175265 HEK293_OSMI2_2hA HEK293_TMG_2hB GOLGA8A protein_coding   11.02785 6.546034 10.39775 1.473182 0.2663785 0.6667622 0.6843193 0.4818219 0.9435201 0.1015367 0.2878291 0.9551275 0.06356667 0.07406667 0.0903000 0.01623333 8.903203e-01 9.212412e-17 FALSE TRUE
MSTRG.10452.5 ENSG00000175265 HEK293_OSMI2_2hA HEK293_TMG_2hB GOLGA8A protein_coding   11.02785 6.546034 10.39775 1.473182 0.2663785 0.6667622 1.0610687 0.0000000 1.8074840 0.0000000 0.1652068 7.5057989 0.08685833 0.00000000 0.1741667 0.17416667 9.212412e-17 9.212412e-17 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000175265 E001 0.5931727 0.8628147904 2.844340e-01 4.246705e-01 15 34379068 34379070 3 - 0.000 0.357 11.755
ENSG00000175265 E002 229.6205618 0.0092474104 5.848619e-05 3.239181e-04 15 34379071 34379956 886 - 2.245 2.447 0.673
ENSG00000175265 E003 100.2957382 0.0009587536 5.189087e-06 3.679243e-05 15 34379957 34380303 347 - 1.880 2.076 0.658
ENSG00000175265 E004 173.9327269 0.0002366838 7.827745e-02 1.567063e-01 15 34380304 34381114 811 - 2.243 2.228 -0.051
ENSG00000175265 E005 42.7760034 0.0165964345 4.901853e-01 6.275395e-01 15 34381115 34381197 83 - 1.657 1.625 -0.109
ENSG00000175265 E006 28.8964586 0.0188366044 2.913602e-01 4.322218e-01 15 34381198 34381206 9 - 1.511 1.437 -0.251
ENSG00000175265 E007 30.5133844 0.0025057853 3.417399e-01 4.856651e-01 15 34381207 34381274 68 - 1.511 1.475 -0.124
ENSG00000175265 E008 9.8110148 0.0023335162 2.162582e-01 3.459041e-01 15 34381275 34381306 32 - 0.932 1.088 0.575
ENSG00000175265 E009 5.3196792 0.0037598657 1.552808e-02 4.168800e-02 15 34381307 34381307 1 - 0.570 0.923 1.440
ENSG00000175265 E010 5.8335461 0.0029237691 3.139038e-02 7.466414e-02 15 34381308 34381309 2 - 0.636 0.941 1.218
ENSG00000175265 E011 10.6329957 0.0016649180 2.756530e-01 4.149943e-01 15 34381310 34381366 57 - 0.976 1.112 0.498
ENSG00000175265 E012 9.9277272 0.0078558103 2.075568e-01 3.355497e-01 15 34381367 34381386 20 - 1.085 0.975 -0.405
ENSG00000175265 E013 31.9476442 0.0100164526 2.438436e-02 6.061201e-02 15 34381387 34381613 227 - 1.574 1.435 -0.476
ENSG00000175265 E014 8.1369704 0.0035690250 6.806155e-02 1.401482e-01 15 34381701 34381856 156 - 1.028 0.844 -0.693
ENSG00000175265 E015 1.2942435 0.0124657699 6.285840e-01 7.441165e-01 15 34381968 34382068 101 - 0.280 0.381 0.635
ENSG00000175265 E016 0.0000000       15 34382151 34382251 101 -      
ENSG00000175265 E017 0.0000000       15 34382386 34382526 141 -      
ENSG00000175265 E018 0.0000000       15 34382527 34382529 3 -      
ENSG00000175265 E019 0.0000000       15 34382797 34383053 257 -      
ENSG00000175265 E020 0.0000000       15 34383441 34383528 88 -      
ENSG00000175265 E021 0.0000000       15 34383746 34383853 108 -      
ENSG00000175265 E022 0.2966881 0.0290785164 2.262659e-01   15 34383961 34384047 87 - 0.205 0.000 -14.705
ENSG00000175265 E023 0.4355181 0.6322948789 3.068547e-01 4.490330e-01 15 34385048 34385157 110 - 0.279 0.000 -14.177
ENSG00000175265 E024 0.0000000       15 34385254 34385338 85 -      
ENSG00000175265 E025 1.0425076 0.0379223708 3.720103e-01 5.164835e-01 15 34385427 34385474 48 - 0.205 0.381 1.217
ENSG00000175265 E026 2.3313560 0.0061027262 2.619449e-01 3.996625e-01 15 34385740 34385778 39 - 0.604 0.440 -0.785
ENSG00000175265 E027 7.7738226 0.0239890075 6.109774e-01 7.302103e-01 15 34386625 34386741 117 - 0.961 0.923 -0.144
ENSG00000175265 E028 16.7408422 0.0153017525 1.058403e-01 1.993590e-01 15 34386742 34386911 170 - 1.308 1.178 -0.457
ENSG00000175265 E029 10.8279176 0.0034396934 5.334855e-01 6.656824e-01 15 34386912 34386957 46 - 1.085 1.049 -0.134
ENSG00000175265 E030 3.5646259 0.0283412735 9.791075e-01 9.909869e-01 15 34386958 34387125 168 - 0.635 0.656 0.090
ENSG00000175265 E031 0.1451727 0.0426970668 5.306953e-01   15 34387126 34387263 138 - 0.115 0.000 -13.658
ENSG00000175265 E032 0.0000000       15 34387264 34387550 287 -      
ENSG00000175265 E033 0.0000000       15 34387551 34387832 282 -      
ENSG00000175265 E034 0.0000000       15 34387833 34387952 120 -      
ENSG00000175265 E035 0.1451727 0.0426970668 5.306953e-01   15 34389644 34389768 125 - 0.115 0.000 -13.658
ENSG00000175265 E036 1.4749052 0.0727155731 4.285330e-01 5.712731e-01 15 34391123 34391265 143 - 0.449 0.314 -0.774
ENSG00000175265 E037 1.8626276 0.0108017037 4.492101e-01 5.904046e-01 15 34391266 34391352 87 - 0.493 0.381 -0.589
ENSG00000175265 E038 3.6118699 0.0044975619 2.834419e-01 4.235465e-01 15 34391353 34391426 74 - 0.718 0.581 -0.590
ENSG00000175265 E039 10.4215425 0.0154692968 4.038241e-03 1.324915e-02 15 34398634 34398729 96 - 1.182 0.866 -1.160
ENSG00000175265 E040 11.4608159 0.0018570246 2.003667e-03 7.237431e-03 15 34399155 34399203 49 - 1.207 0.923 -1.034
ENSG00000175265 E041 8.6854210 0.0025348553 4.435290e-02 9.902797e-02 15 34400712 34400759 48 - 1.063 0.865 -0.741
ENSG00000175265 E042 2.2069951 0.1241710833 9.090366e-01 9.462166e-01 15 34404917 34404969 53 - 0.493 0.497 0.023
ENSG00000175265 E043 5.2955360 0.0469955124 1.110516e-01 2.070851e-01 15 34404970 34405055 86 - 0.883 0.661 -0.891
ENSG00000175265 E044 3.6860817 0.0053979250 3.588371e-02 8.335759e-02 15 34406674 34406676 3 - 0.788 0.492 -1.285
ENSG00000175265 E045 14.9740662 0.0013318328 2.123469e-05 1.308627e-04 15 34406677 34406870 194 - 1.345 0.990 -1.269
ENSG00000175265 E046 4.1634039 0.0045125516 1.019902e-02 2.925587e-02 15 34407524 34407575 52 - 0.847 0.492 -1.519
ENSG00000175265 E047 16.1032266 0.0011651381 6.905423e-04 2.864551e-03 15 34407576 34407735 160 - 1.339 1.075 -0.937
ENSG00000175265 E048 6.2273012 0.0357129236 1.679414e-01 2.855242e-01 15 34428615 34428704 90 - 0.916 0.752 -0.639
ENSG00000175265 E049 0.2214452 0.0353966359 4.489765e-01   15 34428705 34428925 221 - 0.000 0.131 13.052
ENSG00000175265 E050 15.9355072 0.0082036952 4.257187e-01 5.686345e-01 15 34435383 34435471 89 - 1.246 1.198 -0.168
ENSG00000175265 E051 13.7493251 0.0617177955 5.681393e-01 6.948468e-01 15 34437398 34437997 600 - 1.190 1.133 -0.203