ENSG00000175221

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000325464 ENSG00000175221 HEK293_OSMI2_2hA HEK293_TMG_2hB MED16 protein_coding protein_coding 41.69124 80.06145 22.33428 5.08941 0.3812407 -1.841382 1.252628 2.115606 2.3124402 1.3712024 2.3124402 0.1277670 0.03775417 0.02456667 0.10500000 0.08043333 0.72152562 9.741472e-18 FALSE TRUE
ENST00000395808 ENSG00000175221 HEK293_OSMI2_2hA HEK293_TMG_2hB MED16 protein_coding protein_coding 41.69124 80.06145 22.33428 5.08941 0.3812407 -1.841382 4.270717 7.716106 1.7107191 1.0817938 0.2464438 -2.1667299 0.11042500 0.09583333 0.07630000 -0.01953333 0.59016913 9.741472e-18 FALSE TRUE
MSTRG.16016.12 ENSG00000175221 HEK293_OSMI2_2hA HEK293_TMG_2hB MED16 protein_coding   41.69124 80.06145 22.33428 5.08941 0.3812407 -1.841382 12.637497 18.619152 9.7683266 3.0766060 0.9722220 -0.9299025 0.34070833 0.23156667 0.43906667 0.20750000 0.01835800 9.741472e-18 FALSE TRUE
MSTRG.16016.13 ENSG00000175221 HEK293_OSMI2_2hA HEK293_TMG_2hB MED16 protein_coding   41.69124 80.06145 22.33428 5.08941 0.3812407 -1.841382 6.430065 13.905954 3.0680617 0.9880235 0.1104335 -2.1766457 0.13841250 0.17356667 0.13733333 -0.03623333 0.05201918 9.741472e-18 FALSE TRUE
MSTRG.16016.3 ENSG00000175221 HEK293_OSMI2_2hA HEK293_TMG_2hB MED16 protein_coding   41.69124 80.06145 22.33428 5.08941 0.3812407 -1.841382 8.008898 12.509245 4.5634467 0.9883740 2.3444591 -1.4527938 0.21183750 0.15650000 0.20233333 0.04583333 0.97799011 9.741472e-18 FALSE TRUE
MSTRG.16016.4 ENSG00000175221 HEK293_OSMI2_2hA HEK293_TMG_2hB MED16 protein_coding   41.69124 80.06145 22.33428 5.08941 0.3812407 -1.841382 7.177426 20.857887 0.5696061 3.2647234 0.5696061 -5.1700678 0.12095833 0.26320000 0.02466667 -0.23853333 0.03753766 9.741472e-18 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000175221 E001 8.0268256 0.0563933335 1.962369e-01 3.215180e-01 19 867630 867852 223 - 1.020 0.813 -0.787
ENSG00000175221 E002 10.9119788 0.0030096195 7.986406e-01 8.715191e-01 19 867853 867961 109 - 0.933 0.966 0.124
ENSG00000175221 E003 11.7942493 0.0019637080 3.486833e-01 4.929164e-01 19 867962 867962 1 - 0.900 1.017 0.440
ENSG00000175221 E004 16.2851739 0.0221410033 1.409261e-01 2.492952e-01 19 867963 867963 1 - 0.963 1.157 0.704
ENSG00000175221 E005 19.2972336 0.0740228465 2.881091e-01 4.286542e-01 19 867964 867964 1 - 1.068 1.222 0.552
ENSG00000175221 E006 28.7825823 0.0067611473 9.780044e-02 1.871517e-01 19 867965 867966 2 - 1.224 1.383 0.554
ENSG00000175221 E007 49.3279626 0.0322126638 4.953920e-02 1.083487e-01 19 867967 867976 10 - 1.407 1.621 0.732
ENSG00000175221 E008 54.7012845 0.0341417580 2.250756e-02 5.673595e-02 19 867977 867978 2 - 1.418 1.670 0.861
ENSG00000175221 E009 502.0967574 0.0041270159 4.973340e-08 5.364598e-07 19 867979 868251 273 - 2.408 2.619 0.703
ENSG00000175221 E010 529.5363740 0.0016456886 1.681514e-10 2.856508e-09 19 868252 868410 159 - 2.452 2.638 0.621
ENSG00000175221 E011 215.0578968 0.0027657543 3.805211e-04 1.699321e-03 19 868411 868415 5 - 2.093 2.244 0.505
ENSG00000175221 E012 390.7710348 0.0001806320 1.443513e-06 1.158768e-05 19 868416 868499 84 - 2.377 2.495 0.394
ENSG00000175221 E013 363.6663459 0.0001634999 1.250239e-03 4.810781e-03 19 868863 868946 84 - 2.374 2.455 0.270
ENSG00000175221 E014 593.8919374 0.0004815010 1.216060e-06 9.918159e-06 19 871037 871253 217 - 2.566 2.673 0.356
ENSG00000175221 E015 0.1472490 0.0428273545 1.167639e-01   19 871606 871662 57 - 0.212 0.000 -11.238
ENSG00000175221 E016 599.7054199 0.0004496915 3.761472e-04 1.681889e-03 19 871926 872118 193 - 2.596 2.673 0.256
ENSG00000175221 E017 0.3729606 0.0298368792 3.353570e-01 4.791352e-01 19 872913 873009 97 - 0.212 0.068 -1.906
ENSG00000175221 E018 364.9140751 0.0001794109 1.169483e-01 2.156044e-01 19 873449 873535 87 - 2.411 2.450 0.131
ENSG00000175221 E019 252.0733713 0.0003176701 9.213205e-01 9.543279e-01 19 873536 873582 47 - 2.283 2.282 -0.004
ENSG00000175221 E020 284.0147204 0.0004554272 7.619931e-02 1.534280e-01 19 875244 875331 88 - 2.372 2.326 -0.155
ENSG00000175221 E021 281.6958891 0.0004034081 2.739326e-01 4.130344e-01 19 875332 875454 123 - 2.357 2.329 -0.093
ENSG00000175221 E022 376.6985483 0.0001391978 5.196076e-03 1.646342e-02 19 876974 877180 207 - 2.401 2.469 0.227
ENSG00000175221 E023 533.6122956 0.0001278783 9.635938e-01 9.810487e-01 19 879937 880148 212 - 2.607 2.609 0.008
ENSG00000175221 E024 500.4048773 0.0003018704 5.861555e-03 1.824934e-02 19 881559 881714 156 - 2.625 2.571 -0.180
ENSG00000175221 E025 359.4296026 0.0011760379 1.467901e-02 3.976740e-02 19 884903 885008 106 - 2.490 2.427 -0.212
ENSG00000175221 E026 253.6584783 0.0002618943 4.626489e-03 1.489286e-02 19 885770 885821 52 - 2.348 2.272 -0.256
ENSG00000175221 E027 570.2786603 0.0029236870 1.869792e-06 1.464351e-05 19 885822 886201 380 - 2.749 2.613 -0.453
ENSG00000175221 E028 424.6212702 0.0028101352 3.697589e-05 2.148632e-04 19 889638 889807 170 - 2.614 2.488 -0.418
ENSG00000175221 E029 434.1160013 0.0001651400 8.420565e-15 2.801635e-13 19 890137 890244 108 - 2.644 2.489 -0.517
ENSG00000175221 E030 6.7408349 0.0024160483 4.913803e-01 6.286502e-01 19 890245 890423 179 - 0.864 0.769 -0.372
ENSG00000175221 E031 245.4439919 0.0002000007 9.373693e-05 4.925303e-04 19 890963 890983 21 - 2.359 2.254 -0.352
ENSG00000175221 E032 435.9494170 0.0001571624 5.890318e-09 7.594278e-08 19 890984 891149 166 - 2.617 2.500 -0.391
ENSG00000175221 E033 6.0969223 0.0605600386 8.847610e-01 9.303291e-01 19 892347 893085 739 - 0.781 0.762 -0.075
ENSG00000175221 E034 211.1841813 0.0009354632 4.295876e-04 1.888350e-03 19 893086 893218 133 - 2.297 2.187 -0.370