ENSG00000175213

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000311764 ENSG00000175213 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF408 protein_coding protein_coding 18.38672 31.6598 13.4893 1.043102 0.4069213 -1.230223 15.967284 29.336279 10.731644 1.2781622 0.09698745 -1.4499628 0.84428333 0.92603333 0.79733333 -0.12870000 2.245645e-03 9.063245e-05 FALSE TRUE
ENST00000531866 ENSG00000175213 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF408 protein_coding retained_intron 18.38672 31.6598 13.4893 1.043102 0.4069213 -1.230223 1.069719 1.075611 1.256286 0.1131758 0.11436898 0.2220958 0.07029583 0.03426667 0.09283333 0.05856667 9.063245e-05 9.063245e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000175213 E001 9.078650 0.0137982065 1.170885e-02 3.283753e-02 11 46701030 46701030 1 + 1.145 0.850 -1.093
ENSG00000175213 E002 92.010607 0.0004180318 3.144431e-01 4.571571e-01 11 46701031 46701099 69 + 1.914 1.881 -0.111
ENSG00000175213 E003 2.138009 0.0068321356 3.756153e-02 8.651469e-02 11 46701208 46701398 191 + 0.679 0.338 -1.683
ENSG00000175213 E004 225.227733 0.0008817156 1.104137e-01 2.061451e-01 11 46701399 46701676 278 + 2.303 2.268 -0.116
ENSG00000175213 E005 7.567075 0.0076279185 8.993462e-03 2.626357e-02 11 46701677 46701903 227 + 1.091 0.784 -1.157
ENSG00000175213 E006 77.734307 0.0033152271 3.550302e-01 4.993794e-01 11 46702704 46702714 11 + 1.849 1.813 -0.122
ENSG00000175213 E007 127.324530 0.0003899277 1.286887e-01 2.323314e-01 11 46702715 46702765 51 + 2.064 2.020 -0.147
ENSG00000175213 E008 9.082314 0.0099000807 8.912105e-05 4.709019e-04 11 46702766 46702983 218 + 1.235 0.798 -1.616
ENSG00000175213 E009 248.687501 0.0008753220 1.028660e-01 1.948489e-01 11 46702984 46703243 260 + 2.346 2.312 -0.114
ENSG00000175213 E010 973.290707 0.0007756410 1.838330e-06 1.441558e-05 11 46704353 46705912 1560 + 2.864 2.928 0.213