ENSG00000175066

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000392993 ENSG00000175066 HEK293_OSMI2_2hA HEK293_TMG_2hB GK5 protein_coding protein_coding 4.438172 1.733078 6.057958 0.2389272 0.2557123 1.799574 1.95030996 0.49298935 2.98739941 0.07937349 0.06067330 2.57511158 0.41969167 0.30036667 0.49450000 0.19413333 1.402411e-01 4.931282e-05 FALSE TRUE
ENST00000460544 ENSG00000175066 HEK293_OSMI2_2hA HEK293_TMG_2hB GK5 protein_coding retained_intron 4.438172 1.733078 6.057958 0.2389272 0.2557123 1.799574 0.28460959 0.00000000 0.23889327 0.00000000 0.12392522 4.63745531 0.06571250 0.00000000 0.04070000 0.04070000 4.617554e-01 4.931282e-05 FALSE TRUE
ENST00000466685 ENSG00000175066 HEK293_OSMI2_2hA HEK293_TMG_2hB GK5 protein_coding processed_transcript 4.438172 1.733078 6.057958 0.2389272 0.2557123 1.799574 0.05665781 0.11920976 0.08095053 0.08754870 0.08095053 -0.50656108 0.02010417 0.07576667 0.01246667 -0.06330000 4.588987e-01 4.931282e-05 FALSE TRUE
ENST00000472759 ENSG00000175066 HEK293_OSMI2_2hA HEK293_TMG_2hB GK5 protein_coding processed_transcript 4.438172 1.733078 6.057958 0.2389272 0.2557123 1.799574 0.05433714 0.24169471 0.00000000 0.24169471 0.00000000 -4.65360297 0.03246250 0.11036667 0.00000000 -0.11036667 5.753673e-01 4.931282e-05 FALSE TRUE
ENST00000480757 ENSG00000175066 HEK293_OSMI2_2hA HEK293_TMG_2hB GK5 protein_coding nonsense_mediated_decay 4.438172 1.733078 6.057958 0.2389272 0.2557123 1.799574 0.25399045 0.08378438 0.45439570 0.08378438 0.06211886 2.30793506 0.05534167 0.05153333 0.07596667 0.02443333 5.837267e-01 4.931282e-05 FALSE TRUE
ENST00000486459 ENSG00000175066 HEK293_OSMI2_2hA HEK293_TMG_2hB GK5 protein_coding protein_coding 4.438172 1.733078 6.057958 0.2389272 0.2557123 1.799574 0.33560998 0.27542143 0.17600438 0.11318159 0.11060339 -0.61775703 0.08432500 0.14903333 0.02843333 -0.12060000 1.033562e-01 4.931282e-05 FALSE TRUE
ENST00000492097 ENSG00000175066 HEK293_OSMI2_2hA HEK293_TMG_2hB GK5 protein_coding nonsense_mediated_decay 4.438172 1.733078 6.057958 0.2389272 0.2557123 1.799574 0.18149440 0.15928544 0.16245753 0.08005804 0.03033301 0.02678318 0.05436250 0.09266667 0.02733333 -0.06533333 3.702988e-01 4.931282e-05 TRUE TRUE
MSTRG.23841.2 ENSG00000175066 HEK293_OSMI2_2hA HEK293_TMG_2hB GK5 protein_coding   4.438172 1.733078 6.057958 0.2389272 0.2557123 1.799574 0.11029694 0.13623825 0.14434529 0.13623825 0.14434529 0.07784072 0.02418750 0.08380000 0.02226667 -0.06153333 8.716167e-01 4.931282e-05 FALSE TRUE
MSTRG.23841.3 ENSG00000175066 HEK293_OSMI2_2hA HEK293_TMG_2hB GK5 protein_coding   4.438172 1.733078 6.057958 0.2389272 0.2557123 1.799574 0.02813083 0.08206057 0.00000000 0.08206057 0.00000000 -3.20258332 0.01779583 0.05933333 0.00000000 -0.05933333 5.204088e-01 4.931282e-05 FALSE TRUE
MSTRG.23841.8 ENSG00000175066 HEK293_OSMI2_2hA HEK293_TMG_2hB GK5 protein_coding   4.438172 1.733078 6.057958 0.2389272 0.2557123 1.799574 0.71251672 0.05743660 1.33199442 0.05743660 0.04350384 4.31470311 0.11589167 0.02623333 0.22023333 0.19400000 4.931282e-05 4.931282e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000175066 E001 81.6612653 0.0250594009 1.111910e-03 4.343528e-03 3 142157527 142159079 1553 - 1.745 2.051 1.031
ENSG00000175066 E002 11.0956815 0.0020969474 1.740896e-02 4.584224e-02 3 142159080 142159170 91 - 0.919 1.202 1.031
ENSG00000175066 E003 176.6301374 0.0002367980 8.816180e-10 1.320524e-08 3 142159171 142163569 4399 - 2.187 2.008 -0.598
ENSG00000175066 E004 8.2995446 0.0040848822 6.162584e-02 1.293581e-01 3 142163570 142163571 2 - 0.927 0.658 -1.069
ENSG00000175066 E005 15.5182000 0.0016281909 7.439304e-04 3.057190e-03 3 142163572 142163818 247 - 1.189 0.776 -1.540
ENSG00000175066 E006 4.6565622 0.0034408422 1.873786e-01 3.104383e-01 3 142163819 142163822 4 - 0.714 0.496 -0.970
ENSG00000175066 E007 5.6757658 0.0032611576 1.873245e-02 4.874802e-02 3 142163823 142163908 86 - 0.804 0.384 -1.917
ENSG00000175066 E008 2.7313626 0.0067842578 1.170865e-01 2.157878e-01 3 142163909 142163918 10 - 0.548 0.233 -1.834
ENSG00000175066 E009 85.5778992 0.0004519892 3.882824e-02 8.886398e-02 3 142163919 142165565 1647 - 1.857 1.783 -0.250
ENSG00000175066 E010 17.2770367 0.0010862441 1.439309e-04 7.206322e-04 3 142165566 142165637 72 - 1.233 0.776 -1.695
ENSG00000175066 E011 19.9613061 0.0009445609 1.582294e-02 4.234209e-02 3 142165638 142165698 61 - 1.269 1.039 -0.823
ENSG00000175066 E012 19.6694975 0.0009416952 2.748485e-01 4.140661e-01 3 142165699 142165770 72 - 1.244 1.161 -0.294
ENSG00000175066 E013 1.6575400 0.0085574681 2.409471e-02 6.000776e-02 3 142170183 142170324 142 - 0.242 0.658 2.253
ENSG00000175066 E014 23.1727691 0.0168857366 6.632374e-01 7.713456e-01 3 142170325 142170458 134 - 1.297 1.273 -0.085
ENSG00000175066 E015 15.8484088 0.0299056052 7.367124e-01 8.271233e-01 3 142171419 142171478 60 - 1.141 1.117 -0.090
ENSG00000175066 E016 20.3795010 0.0214080006 7.729631e-01 8.534733e-01 3 142172353 142172456 104 - 1.226 1.275 0.173
ENSG00000175066 E017 0.5085815 0.3529402147 1.155987e-01 2.136963e-01 3 142173076 142173197 122 - 0.060 0.386 3.265
ENSG00000175066 E018 21.4024384 0.0172311739 8.818968e-01 9.283304e-01 3 142177482 142177576 95 - 1.258 1.258 0.000
ENSG00000175066 E019 1.5500344 0.0087974040 8.848559e-01 9.303915e-01 3 142177577 142177605 29 - 0.341 0.384 0.253
ENSG00000175066 E020 14.0448694 0.0661138504 7.432416e-01 8.319392e-01 3 142181461 142181461 1 - 1.095 1.047 -0.173
ENSG00000175066 E021 25.2776896 0.0184146775 7.176001e-01 8.129256e-01 3 142181462 142181565 104 - 1.330 1.308 -0.079
ENSG00000175066 E022 20.9659820 0.0156014579 1.206898e-01 2.209498e-01 3 142182923 142183049 127 - 1.279 1.117 -0.574
ENSG00000175066 E023 1.4112044 0.0099980154 7.633535e-01 8.466736e-01 3 142183050 142183488 439 - 0.310 0.384 0.445
ENSG00000175066 E024 15.0408458 0.0012581366 4.062661e-05 2.337612e-04 3 142184711 142185793 1083 - 0.998 1.401 1.433
ENSG00000175066 E025 21.2006360 0.0008948708 2.719619e-01 4.107790e-01 3 142185929 142185989 61 - 1.229 1.349 0.418
ENSG00000175066 E026 0.4502799 0.0326968049 7.165234e-01 8.121672e-01 3 142186166 142186193 28 - 0.161 0.000 -8.928
ENSG00000175066 E027 15.6273360 0.0194790265 8.477910e-01 9.054807e-01 3 142186194 142186267 74 - 1.126 1.120 -0.021
ENSG00000175066 E028 20.4858315 0.0008730222 5.172957e-02 1.122374e-01 3 142186452 142186513 62 - 1.198 1.388 0.667
ENSG00000175066 E029 21.4414626 0.0008613303 3.318352e-01 4.754698e-01 3 142187704 142187779 76 - 1.240 1.348 0.378
ENSG00000175066 E030 19.0299491 0.0009979059 6.496400e-01 7.608519e-01 3 142198802 142198856 55 - 1.221 1.202 -0.070
ENSG00000175066 E031 19.8819665 0.0011036236 6.313000e-01 7.463207e-01 3 142198857 142198933 77 - 1.221 1.289 0.238
ENSG00000175066 E032 1.6554663 0.0084416477 9.992841e-01 1.000000e+00 3 142204217 142204272 56 - 0.370 0.384 0.082
ENSG00000175066 E033 0.7750806 0.0205572437 9.299452e-01 9.599057e-01 3 142204273 142204296 24 - 0.203 0.233 0.253
ENSG00000175066 E034 0.7750806 0.0205572437 9.299452e-01 9.599057e-01 3 142204297 142204311 15 - 0.203 0.233 0.253
ENSG00000175066 E035 1.8568642 0.4739308247 3.288321e-01 4.723278e-01 3 142204312 142204602 291 - 0.339 0.582 1.255
ENSG00000175066 E036 0.7279398 0.2363221582 4.675876e-01 6.070959e-01 3 142204603 142204674 72 - 0.241 0.001 -8.811
ENSG00000175066 E037 0.8021360 0.6159995070 7.068692e-01 8.049424e-01 3 142204675 142204694 20 - 0.202 0.240 0.320
ENSG00000175066 E038 22.1715940 0.0018158397 6.150652e-01 7.333980e-01 3 142204695 142204788 94 - 1.266 1.334 0.239
ENSG00000175066 E039 18.3723813 0.0010543835 5.029621e-03 1.601261e-02 3 142213526 142213601 76 - 1.131 1.400 0.947
ENSG00000175066 E040 14.5806143 0.0021721925 2.840336e-03 9.795266e-03 3 142215599 142215688 90 - 1.023 1.334 1.110
ENSG00000175066 E041 6.4140425 0.0025568629 2.006511e-03 7.246460e-03 3 142215689 142215692 4 - 0.675 1.092 1.608
ENSG00000175066 E042 22.0964844 0.0011217238 2.066238e-02 5.287407e-02 3 142225309 142225599 291 - 1.221 1.436 0.748