ENSG00000174996

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000316924 ENSG00000174996 HEK293_OSMI2_2hA HEK293_TMG_2hB KLC2 protein_coding protein_coding 61.41175 84.73953 44.15413 6.042729 1.009113 -0.9403303 6.522589 10.389222 4.601009 1.1897626 0.5046585 -1.1733212 0.10022917 0.12210000 0.10413333 -0.01796667 6.189739e-01 2.591163e-19 FALSE TRUE
ENST00000394065 ENSG00000174996 HEK293_OSMI2_2hA HEK293_TMG_2hB KLC2 protein_coding protein_coding 61.41175 84.73953 44.15413 6.042729 1.009113 -0.9403303 2.913860 1.971372 2.861282 0.3738190 0.3883312 0.5351958 0.05272917 0.02296667 0.06446667 0.04150000 8.090222e-05 2.591163e-19 FALSE TRUE
ENST00000394067 ENSG00000174996 HEK293_OSMI2_2hA HEK293_TMG_2hB KLC2 protein_coding protein_coding 61.41175 84.73953 44.15413 6.042729 1.009113 -0.9403303 24.446493 43.091803 12.965100 3.0697958 0.2445416 -1.7320025 0.36735417 0.50883333 0.29370000 -0.21513333 2.591163e-19 2.591163e-19 FALSE TRUE
ENST00000421552 ENSG00000174996 HEK293_OSMI2_2hA HEK293_TMG_2hB KLC2 protein_coding protein_coding 61.41175 84.73953 44.15413 6.042729 1.009113 -0.9403303 2.997776 3.682749 3.170105 2.2595431 0.1522647 -0.2156209 0.05025417 0.04030000 0.07173333 0.03143333 6.905866e-01 2.591163e-19 FALSE TRUE
ENST00000534023 ENSG00000174996 HEK293_OSMI2_2hA HEK293_TMG_2hB KLC2 protein_coding retained_intron 61.41175 84.73953 44.15413 6.042729 1.009113 -0.9403303 3.946144 0.891064 2.305267 0.5116128 0.7340367 1.3614772 0.07372500 0.01076667 0.05293333 0.04216667 3.477552e-01 2.591163e-19 FALSE TRUE
MSTRG.5759.8 ENSG00000174996 HEK293_OSMI2_2hA HEK293_TMG_2hB KLC2 protein_coding   61.41175 84.73953 44.15413 6.042729 1.009113 -0.9403303 13.122057 15.509774 11.104055 2.4503460 0.7873549 -0.4817223 0.22859167 0.18193333 0.25126667 0.06933333 1.761647e-01 2.591163e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000174996 E001 1.7444242 0.0083745011 3.470364e-03 1.164020e-02 11 66250295 66250339 45 + 0.700 0.213 -2.659
ENSG00000174996 E002 2.2842111 0.0072678225 4.910034e-01 6.283165e-01 11 66257294 66257488 195 + 0.545 0.430 -0.563
ENSG00000174996 E003 0.1817044 0.0392589601 1.000000e+00   11 66257620 66257629 10 + 0.000 0.083 9.014
ENSG00000174996 E004 1.0631447 0.0120095250 8.213893e-02 1.628978e-01 11 66257630 66257658 29 + 0.000 0.356 11.599
ENSG00000174996 E005 13.4856246 0.0075498750 1.325521e-01 2.376282e-01 11 66257659 66257702 44 + 1.205 1.057 -0.530
ENSG00000174996 E006 15.1087224 0.0058965058 3.901933e-02 8.923124e-02 11 66257703 66257718 16 + 1.279 1.088 -0.680
ENSG00000174996 E007 24.0190267 0.0174431514 1.615546e-02 4.307068e-02 11 66257719 66257730 12 + 1.485 1.266 -0.759
ENSG00000174996 E008 123.0106270 0.0214580831 1.662525e-01 2.833270e-01 11 66257731 66257807 77 + 2.095 2.010 -0.287
ENSG00000174996 E009 223.3645361 0.0121512163 3.069516e-01 4.491262e-01 11 66257808 66257871 64 + 2.322 2.280 -0.138
ENSG00000174996 E010 40.2989353 0.0209689749 6.848173e-07 5.892029e-06 11 66258036 66258544 509 + 1.823 1.396 -1.457
ENSG00000174996 E011 12.4396521 0.0016212880 4.096973e-06 2.971627e-05 11 66258545 66258546 2 + 1.343 0.913 -1.548
ENSG00000174996 E012 70.6578476 0.0010086639 6.196559e-05 3.411466e-04 11 66258547 66258583 37 + 1.914 1.736 -0.602
ENSG00000174996 E013 215.0346480 0.0113762891 8.455185e-02 1.666763e-01 11 66258584 66258592 9 + 2.333 2.252 -0.270
ENSG00000174996 E014 511.5174056 0.0081591818 2.019929e-01 3.286917e-01 11 66258593 66258822 230 + 2.681 2.640 -0.136
ENSG00000174996 E015 68.7349961 0.0061181372 4.158325e-21 3.031097e-19 11 66260913 66261741 829 + 2.100 1.573 -1.778
ENSG00000174996 E016 305.1911491 0.0034059111 3.212300e-01 4.644686e-01 11 66261742 66261835 94 + 2.442 2.419 -0.077
ENSG00000174996 E017 349.2714211 0.0018410847 2.767889e-01 4.162644e-01 11 66261836 66261929 94 + 2.499 2.478 -0.071
ENSG00000174996 E018 274.5017392 0.0012765505 3.952742e-02 9.015376e-02 11 66261930 66261972 43 + 2.416 2.366 -0.168
ENSG00000174996 E019 350.6027458 0.0002248077 9.389993e-04 3.750671e-03 11 66262123 66262192 70 + 2.531 2.467 -0.213
ENSG00000174996 E020 18.7647061 0.0035381514 1.677434e-07 1.631925e-06 11 66262193 66262655 463 + 1.506 1.080 -1.495
ENSG00000174996 E021 496.7674826 0.0004365831 1.049154e-01 1.979711e-01 11 66262814 66262917 104 + 2.652 2.628 -0.080
ENSG00000174996 E022 599.9409187 0.0001100496 1.675386e-07 1.630156e-06 11 66262918 66263036 119 + 2.772 2.695 -0.256
ENSG00000174996 E023 4.0317835 0.0086799613 7.424218e-03 2.232179e-02 11 66263037 66263062 26 + 0.904 0.522 -1.592
ENSG00000174996 E024 366.1317923 0.0001778736 8.027153e-05 4.291818e-04 11 66263660 66263681 22 + 2.558 2.483 -0.249
ENSG00000174996 E025 549.6546444 0.0004482264 2.762674e-05 1.657158e-04 11 66263682 66263747 66 + 2.732 2.661 -0.237
ENSG00000174996 E026 535.5095624 0.0005061716 8.506733e-03 2.505441e-02 11 66263851 66263910 60 + 2.700 2.657 -0.145
ENSG00000174996 E027 469.3765371 0.0001363873 8.226516e-03 2.435862e-02 11 66263911 66263952 42 + 2.641 2.599 -0.139
ENSG00000174996 E028 768.2153756 0.0001288006 7.934913e-01 8.679450e-01 11 66264046 66264219 174 + 2.823 2.826 0.011
ENSG00000174996 E029 545.0378478 0.0021242374 7.311194e-01 8.228550e-01 11 66264345 66264444 100 + 2.665 2.684 0.065
ENSG00000174996 E030 463.2272479 0.0005004967 7.698275e-03 2.302158e-02 11 66265023 66265072 50 + 2.558 2.623 0.215
ENSG00000174996 E031 457.0983446 0.0003379636 5.459882e-04 2.331894e-03 11 66265168 66265235 68 + 2.541 2.620 0.262
ENSG00000174996 E032 544.6347334 0.0001516618 1.913762e-05 1.192503e-04 11 66265655 66265763 109 + 2.612 2.696 0.280
ENSG00000174996 E033 573.8759242 0.0001153639 9.183198e-08 9.385678e-07 11 66265854 66266012 159 + 2.622 2.722 0.333
ENSG00000174996 E034 568.5645421 0.0015804126 1.996525e-04 9.623094e-04 11 66266093 66266217 125 + 2.617 2.721 0.346
ENSG00000174996 E035 416.2305898 0.0017317981 3.134944e-06 2.335361e-05 11 66266433 66266490 58 + 2.452 2.594 0.475
ENSG00000174996 E036 26.8917231 0.0102744864 4.680974e-04 2.038097e-03 11 66266491 66266872 382 + 1.575 1.291 -0.981
ENSG00000174996 E037 879.0088431 0.0023790784 5.194987e-06 3.682875e-05 11 66266873 66267188 316 + 2.784 2.917 0.441
ENSG00000174996 E038 788.0857159 0.0033168335 7.246141e-07 6.203723e-06 11 66267189 66267860 672 + 2.711 2.876 0.548