ENSG00000174953

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000308361 ENSG00000174953 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX36 protein_coding protein_coding 29.00642 9.345973 42.91088 0.8817982 1.845346 2.19772 4.274641 2.1814586 5.340412 0.16372883 0.6328009 1.287759 0.1602875 0.2365000 0.1242000 -0.112300000 0.007295056 0.006875118 FALSE TRUE
ENST00000329463 ENSG00000174953 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX36 protein_coding protein_coding 29.00642 9.345973 42.91088 0.8817982 1.845346 2.19772 7.543231 0.9586624 12.781821 0.49130540 0.4843937 3.723084 0.2152583 0.1004333 0.2985333 0.198100000 0.397213270 0.006875118 FALSE TRUE
ENST00000495598 ENSG00000174953 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX36 protein_coding retained_intron 29.00642 9.345973 42.91088 0.8817982 1.845346 2.19772 4.630506 2.4383298 5.185388 0.26042150 0.6748934 1.085434 0.2104583 0.2646667 0.1207000 -0.143966667 0.006875118 0.006875118 FALSE TRUE
ENST00000496811 ENSG00000174953 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX36 protein_coding protein_coding 29.00642 9.345973 42.91088 0.8817982 1.845346 2.19772 3.479353 1.3086869 6.266481 0.03784019 0.2138602 2.250854 0.1253208 0.1417333 0.1463000 0.004566667 0.952569977 0.006875118 FALSE TRUE
MSTRG.23945.3 ENSG00000174953 HEK293_OSMI2_2hA HEK293_TMG_2hB DHX36 protein_coding   29.00642 9.345973 42.91088 0.8817982 1.845346 2.19772 6.061773 1.5421223 9.166380 0.18838626 0.7554583 2.563683 0.1877417 0.1672333 0.2153000 0.048066667 0.577354708 0.006875118 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000174953 E001 115.9017881 0.0002608839 3.212286e-04 1.465840e-03 3 154272546 154273660 1115 - 1.946 2.083 0.461
ENSG00000174953 E002 47.0777763 0.0005715202 2.147716e-01 3.441646e-01 3 154273661 154273905 245 - 1.611 1.527 -0.287
ENSG00000174953 E003 26.9586594 0.0050981803 1.839764e-01 3.062022e-01 3 154273906 154274046 141 - 1.389 1.263 -0.439
ENSG00000174953 E004 135.0968883 0.0003520364 8.293470e-03 2.452709e-02 3 154274047 154275667 1621 - 2.065 1.957 -0.363
ENSG00000174953 E005 132.2613332 0.0193190680 7.450397e-05 4.018035e-04 3 154275668 154276028 361 - 1.952 2.259 1.028
ENSG00000174953 E006 130.2292066 0.0006462223 4.500052e-10 7.108491e-09 3 154276029 154276170 142 - 1.970 2.197 0.758
ENSG00000174953 E007 229.9606672 0.0003785776 3.070565e-07 2.834979e-06 3 154276171 154276356 186 - 2.240 2.383 0.476
ENSG00000174953 E008 2.8914228 0.0060233800 8.048272e-01 8.758184e-01 3 154276553 154276746 194 - 0.537 0.489 -0.229
ENSG00000174953 E009 208.2774419 0.0022095541 4.225909e-05 2.422274e-04 3 154276747 154276899 153 - 2.196 2.342 0.489
ENSG00000174953 E010 113.9897090 0.0003479745 3.049523e-06 2.277818e-05 3 154277598 154277624 27 - 1.929 2.107 0.598
ENSG00000174953 E011 104.8009296 0.0028633120 6.163366e-04 2.593353e-03 3 154277625 154277634 10 - 1.899 2.059 0.537
ENSG00000174953 E012 149.2237405 0.0009559286 9.978254e-05 5.208294e-04 3 154277635 154277718 84 - 2.054 2.195 0.470
ENSG00000174953 E013 3.6203947 0.0043054226 1.825386e-01 3.043993e-01 3 154277719 154277894 176 - 0.639 0.378 -1.273
ENSG00000174953 E014 48.4534747 0.0085335941 3.490045e-05 2.041052e-04 3 154278495 154280578 2084 - 1.663 1.295 -1.266
ENSG00000174953 E015 118.1176485 0.0008290028 1.378820e-05 8.867611e-05 3 154280579 154280669 91 - 1.942 2.113 0.572
ENSG00000174953 E016 128.8803505 0.0002868026 1.662500e-04 8.191295e-04 3 154280763 154280862 100 - 1.991 2.128 0.459
ENSG00000174953 E017 153.3367667 0.0002625466 3.792685e-04 1.694486e-03 3 154283188 154283271 84 - 2.070 2.189 0.399
ENSG00000174953 E018 125.1076615 0.0002733111 1.419046e-01 2.506423e-01 3 154284583 154284652 70 - 1.997 2.052 0.185
ENSG00000174953 E019 77.2977146 0.0003668328 4.359741e-01 5.781140e-01 3 154284653 154284669 17 - 1.794 1.830 0.124
ENSG00000174953 E020 0.6235652 0.0192882656 7.126676e-01 8.093291e-01 3 154284781 154284813 33 - 0.164 0.229 0.602
ENSG00000174953 E021 197.1145264 0.0001919764 5.267721e-03 1.666005e-02 3 154284814 154284987 174 - 2.188 2.270 0.277
ENSG00000174953 E022 149.2898047 0.0002399354 1.436514e-01 2.529769e-01 3 154288866 154288964 99 - 2.076 2.126 0.168
ENSG00000174953 E023 131.8704355 0.0002230622 1.346714e-01 2.406464e-01 3 154289709 154289826 118 - 2.021 2.076 0.182
ENSG00000174953 E024 158.5624259 0.0002500853 7.173643e-01 8.127752e-01 3 154292551 154292694 144 - 2.113 2.124 0.037
ENSG00000174953 E025 110.4646768 0.0002863054 4.311670e-01 5.737498e-01 3 154293748 154293812 65 - 1.965 1.929 -0.119
ENSG00000174953 E026 74.5121430 0.0005827418 2.340868e-01 3.672542e-01 3 154295284 154295297 14 - 1.801 1.736 -0.219
ENSG00000174953 E027 84.1148692 0.0012819165 2.200013e-01 3.504199e-01 3 154295298 154295339 42 - 1.853 1.788 -0.219
ENSG00000174953 E028 109.8642964 0.0005812659 3.896448e-01 5.337240e-01 3 154299838 154299925 88 - 1.962 1.923 -0.132
ENSG00000174953 E029 123.1919365 0.0006087412 3.296181e-02 7.772709e-02 3 154300594 154300696 103 - 2.023 1.930 -0.312
ENSG00000174953 E030 173.1341983 0.0002511414 7.774764e-09 9.779601e-08 3 154300987 154301127 141 - 2.189 1.970 -0.732
ENSG00000174953 E031 79.0578586 0.0010643764 4.528937e-05 2.579036e-04 3 154303329 154303343 15 - 1.852 1.616 -0.799
ENSG00000174953 E032 116.3715518 0.0124117973 5.399692e-04 2.309728e-03 3 154303344 154303410 67 - 2.020 1.775 -0.826
ENSG00000174953 E033 175.6704975 0.0002505611 2.268895e-08 2.618393e-07 3 154304806 154304972 167 - 2.195 1.986 -0.699
ENSG00000174953 E034 118.2793067 0.0037906385 4.161283e-04 1.836259e-03 3 154305094 154305168 75 - 2.021 1.831 -0.639
ENSG00000174953 E035 0.1472490 0.0429161890 1.000000e+00   3 154305169 154305337 169 - 0.062 0.000 -8.292
ENSG00000174953 E036 107.8102533 0.0074370168 2.671381e-02 6.539581e-02 3 154306216 154306295 80 - 1.973 1.833 -0.470
ENSG00000174953 E037 144.9211731 0.0046566069 6.491190e-04 2.712973e-03 3 154309653 154309795 143 - 2.107 1.927 -0.604
ENSG00000174953 E038 72.5502876 0.0086764357 7.104074e-02 1.450718e-01 3 154309796 154309823 28 - 1.804 1.670 -0.452
ENSG00000174953 E039 76.7830522 0.0042796479 1.981591e-03 7.169630e-03 3 154311636 154311674 39 - 1.838 1.637 -0.680
ENSG00000174953 E040 0.5244761 0.2634098711 5.128952e-01 6.477721e-01 3 154311675 154312248 574 - 0.116 0.232 1.207
ENSG00000174953 E041 4.1050256 0.0038976913 1.173995e-01 2.162405e-01 3 154314651 154315045 395 - 0.574 0.816 1.016
ENSG00000174953 E042 208.1326812 0.0054167538 8.943253e-06 6.013816e-05 3 154315046 154315280 235 - 2.268 2.048 -0.736
ENSG00000174953 E043 151.6529989 0.0009450236 2.000497e-12 4.670218e-11 3 154316039 154316163 125 - 2.144 1.831 -1.052
ENSG00000174953 E044 0.1451727 0.0424476404 1.000000e+00   3 154319081 154319284 204 - 0.062 0.000 -8.292
ENSG00000174953 E045 95.6532226 0.0037630130 6.730927e-07 5.798790e-06 3 154324174 154324487 314 - 1.946 1.644 -1.020