ENSG00000174903

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000311481 ENSG00000174903 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB1B protein_coding protein_coding 205.2958 381.1177 107.4964 18.42751 1.609616 -1.825852 156.16462 290.65615 75.75936 11.460818 1.201724 -1.939677 0.7510583 0.7633333 0.7049000 -0.05843333 0.018396468 0.003533633 FALSE  
MSTRG.5763.3 ENSG00000174903 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB1B protein_coding   205.2958 381.1177 107.4964 18.42751 1.609616 -1.825852 39.32575 69.00622 27.47292 6.351377 1.475357 -1.328400 0.2019500 0.1805333 0.2553333 0.07480000 0.003533633 0.003533633 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000174903 E001 2.502459 0.0061906997 5.208123e-01 6.547506e-01 11 66268508 66268554 47 + 0.347 0.489 0.766
ENSG00000174903 E002 4.524541 0.0045438496 2.520575e-01 3.883787e-01 11 66268555 66268589 35 + 0.770 0.615 -0.648
ENSG00000174903 E003 357.765044 0.0010546617 3.660812e-05 2.129519e-04 11 66268590 66268638 49 + 2.512 2.429 -0.277
ENSG00000174903 E004 1129.692840 0.0023381910 3.474192e-05 2.032911e-04 11 66268639 66268693 55 + 3.006 2.931 -0.247
ENSG00000174903 E005 14.035195 0.0014227178 2.276116e-03 8.078599e-03 11 66269563 66269694 132 + 1.287 1.015 -0.972
ENSG00000174903 E006 1656.675594 0.0012330886 8.962553e-08 9.178489e-07 11 66271797 66271869 73 + 3.168 3.098 -0.231
ENSG00000174903 E007 2314.432476 0.0009010255 2.943967e-09 4.001440e-08 11 66272157 66272252 96 + 3.307 3.245 -0.204
ENSG00000174903 E008 2400.790875 0.0005095414 8.056629e-06 5.466510e-05 11 66272365 66272460 96 + 3.295 3.268 -0.091
ENSG00000174903 E009 5.642082 0.0333375104 6.847267e-05 3.727569e-04 11 66273006 66273075 70 + 1.122 0.556 -2.235
ENSG00000174903 E010 2606.691760 0.0000481059 8.863786e-01 9.313290e-01 11 66275804 66275935 132 + 3.286 3.312 0.088
ENSG00000174903 E011 1008.666066 0.0008273656 1.181001e-01 2.172121e-01 11 66276044 66276046 3 + 2.848 2.908 0.200
ENSG00000174903 E012 9641.510367 0.0012874236 6.595255e-13 1.668291e-11 11 66276047 66277492 1446 + 3.788 3.897 0.362