ENSG00000174775

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000311189 ENSG00000174775 HEK293_OSMI2_2hA HEK293_TMG_2hB HRAS protein_coding protein_coding 140.8919 275.1703 70.15438 2.295392 2.442668 -1.971566 66.442872 144.19926 30.335054 3.6810674 1.5879006 -2.248630 0.44458333 0.5242000 0.4323333 -0.0918666667 0.02811777 0.01765213 FALSE  
ENST00000417302 ENSG00000174775 HEK293_OSMI2_2hA HEK293_TMG_2hB HRAS protein_coding protein_coding 140.8919 275.1703 70.15438 2.295392 2.442668 -1.971566 7.209369 15.54083 3.358211 0.6561324 0.3467431 -2.206938 0.05099167 0.0565000 0.0483000 -0.0082000000 0.66265281 0.01765213 FALSE  
ENST00000451590 ENSG00000174775 HEK293_OSMI2_2hA HEK293_TMG_2hB HRAS protein_coding protein_coding 140.8919 275.1703 70.15438 2.295392 2.442668 -1.971566 16.921375 40.32884 10.214635 2.3443768 1.1260555 -1.980120 0.11001667 0.1465333 0.1471000 0.0005666667 1.00000000 0.01765213 FALSE  
ENST00000479482 ENSG00000174775 HEK293_OSMI2_2hA HEK293_TMG_2hB HRAS protein_coding retained_intron 140.8919 275.1703 70.15438 2.295392 2.442668 -1.971566 24.655339 40.03849 13.875546 4.0593321 2.3593173 -1.528164 0.19722083 0.1456000 0.1959000 0.0503000000 0.47217599 0.01765213 FALSE  
MSTRG.4901.8 ENSG00000174775 HEK293_OSMI2_2hA HEK293_TMG_2hB HRAS protein_coding   140.8919 275.1703 70.15438 2.295392 2.442668 -1.971566 10.270234 11.15962 4.610169 2.1269885 1.0948494 -1.273563 0.08566250 0.0405000 0.0668000 0.0263000000 0.48964511 0.01765213 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000174775 E001 0.1472490 4.337560e-02 8.584302e-02   11 531239 531932 694 - 0.234 0.000 -10.856
ENSG00000174775 E002 0.2214452 4.036109e-02 1.000000e+00   11 531933 532241 309 - 0.000 0.060 9.126
ENSG00000174775 E003 7.3679557 5.304408e-02 6.397381e-03 1.965561e-02 11 532242 532242 1 - 0.234 0.857 3.117
ENSG00000174775 E004 20.1302901 1.593873e-02 3.061711e-02 7.317906e-02 11 532243 532252 10 - 1.378 1.147 -0.811
ENSG00000174775 E005 86.0477499 1.755791e-02 3.588960e-01 5.032625e-01 11 532253 532261 9 - 1.860 1.782 -0.262
ENSG00000174775 E006 217.6555387 1.112384e-02 3.203613e-01 4.634707e-01 11 532262 532274 13 - 2.245 2.182 -0.213
ENSG00000174775 E007 1668.5930514 1.220386e-04 8.973111e-05 4.737422e-04 11 532275 532522 248 - 3.037 3.078 0.138
ENSG00000174775 E008 452.8853261 1.035748e-03 4.472564e-01 5.886577e-01 11 532523 532630 108 - 2.493 2.507 0.047
ENSG00000174775 E009 733.7188235 1.101662e-04 6.399427e-08 6.747661e-07 11 532631 532641 11 - 2.633 2.729 0.317
ENSG00000174775 E010 1505.9334995 1.484125e-04 5.945825e-10 9.198920e-09 11 532642 532755 114 - 2.963 3.038 0.252
ENSG00000174775 E011 140.9329805 3.407671e-04 7.962257e-01 8.699110e-01 11 533277 533353 77 - 1.995 2.000 0.016
ENSG00000174775 E012 80.2566002 3.723079e-04 2.751377e-01 4.143997e-01 11 533354 533358 5 - 1.810 1.748 -0.211
ENSG00000174775 E013 1876.5617951 1.454238e-04 6.772524e-02 1.395801e-01 11 533453 533612 160 - 3.110 3.125 0.050
ENSG00000174775 E014 852.5394928 9.963735e-05 1.384552e-01 2.459439e-01 11 533766 533794 29 - 2.806 2.776 -0.099
ENSG00000174775 E015 1526.8542967 3.675323e-04 8.908183e-05 4.707077e-04 11 533795 533944 150 - 3.083 3.023 -0.198
ENSG00000174775 E016 14.0938024 2.937722e-03 2.366164e-07 2.235947e-06 11 533945 533976 32 - 1.427 0.924 -1.798
ENSG00000174775 E017 813.8244879 7.875313e-04 6.993711e-04 2.896742e-03 11 534212 534278 67 - 2.822 2.747 -0.247
ENSG00000174775 E018 624.9148127 6.351843e-04 1.349518e-03 5.141626e-03 11 534279 534375 97 - 2.708 2.633 -0.249
ENSG00000174775 E019 18.2097467 6.460901e-03 9.551191e-10 1.421513e-08 11 534518 534690 173 - 1.557 0.996 -1.974
ENSG00000174775 E020 4.0302443 1.011854e-01 1.760913e-02 4.629084e-02 11 535257 535415 159 - 0.946 0.470 -2.006
ENSG00000174775 E021 192.2305112 3.174765e-03 1.710628e-05 1.077443e-04 11 535416 535603 188 - 2.286 2.103 -0.612
ENSG00000174775 E022 0.0000000       11 536336 536852 517 -      
ENSG00000174775 E023 0.0000000       11 536853 537321 469 -