ENSG00000174672

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000308219 ENSG00000174672 HEK293_OSMI2_2hA HEK293_TMG_2hB BRSK2 protein_coding protein_coding 23.92249 29.69491 20.62902 2.847508 0.7698457 -0.5253265 3.4701356 5.237399 4.111922 0.6381193 0.3237939 -0.34828507 0.13952500 0.17610000 0.20026667 0.02416667 7.365822e-01 3.23588e-26 FALSE TRUE
ENST00000526678 ENSG00000174672 HEK293_OSMI2_2hA HEK293_TMG_2hB BRSK2 protein_coding protein_coding 23.92249 29.69491 20.62902 2.847508 0.7698457 -0.5253265 6.4936689 6.851387 5.589590 0.3802640 0.3903226 -0.29317896 0.28755000 0.23326667 0.27200000 0.03873333 6.067025e-01 3.23588e-26 FALSE TRUE
ENST00000526768 ENSG00000174672 HEK293_OSMI2_2hA HEK293_TMG_2hB BRSK2 protein_coding retained_intron 23.92249 29.69491 20.62902 2.847508 0.7698457 -0.5253265 1.7303602 1.070842 1.106580 0.2649469 0.1925665 0.04693173 0.07571250 0.03516667 0.05336667 0.01820000 3.722368e-01 3.23588e-26 TRUE FALSE
ENST00000529951 ENSG00000174672 HEK293_OSMI2_2hA HEK293_TMG_2hB BRSK2 protein_coding nonsense_mediated_decay 23.92249 29.69491 20.62902 2.847508 0.7698457 -0.5253265 1.0050976 1.660078 0.000000 0.6496374 0.0000000 -7.38377139 0.03604583 0.05976667 0.00000000 -0.05976667 1.169784e-05 3.23588e-26 TRUE TRUE
ENST00000531078 ENSG00000174672 HEK293_OSMI2_2hA HEK293_TMG_2hB BRSK2 protein_coding retained_intron 23.92249 29.69491 20.62902 2.847508 0.7698457 -0.5253265 0.4082176 0.000000 1.174633 0.0000000 0.2400838 6.88829589 0.02262917 0.00000000 0.05623333 0.05623333 3.304516e-15 3.23588e-26 TRUE TRUE
ENST00000531932 ENSG00000174672 HEK293_OSMI2_2hA HEK293_TMG_2hB BRSK2 protein_coding retained_intron 23.92249 29.69491 20.62902 2.847508 0.7698457 -0.5253265 3.0135368 2.881433 3.033727 0.4774177 0.3258815 0.07405444 0.12864583 0.09603333 0.14650000 0.05046667 1.040832e-01 3.23588e-26 FALSE FALSE
ENST00000544817 ENSG00000174672 HEK293_OSMI2_2hA HEK293_TMG_2hB BRSK2 protein_coding protein_coding 23.92249 29.69491 20.62902 2.847508 0.7698457 -0.5253265 1.1097467 2.960622 0.000000 0.4309022 0.0000000 -8.21462146 0.03851250 0.09950000 0.00000000 -0.09950000 3.235880e-26 3.23588e-26 TRUE FALSE
MSTRG.4940.7 ENSG00000174672 HEK293_OSMI2_2hA HEK293_TMG_2hB BRSK2 protein_coding   23.92249 29.69491 20.62902 2.847508 0.7698457 -0.5253265 3.5656833 5.808435 2.814543 0.5588322 0.2063444 -1.04261356 0.14154167 0.19636667 0.13613333 -0.06023333 3.690126e-02 3.23588e-26 FALSE TRUE
MSTRG.4940.9 ENSG00000174672 HEK293_OSMI2_2hA HEK293_TMG_2hB BRSK2 protein_coding   23.92249 29.69491 20.62902 2.847508 0.7698457 -0.5253265 2.2017518 2.771293 2.142405 0.9318244 0.2996211 -0.36980641 0.09062083 0.08886667 0.10356667 0.01470000 8.258811e-01 3.23588e-26 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000174672 E001 0.0000000       11 1389867 1389898 32 +      
ENSG00000174672 E002 0.0000000       11 1389899 1389900 2 +      
ENSG00000174672 E003 0.0000000       11 1389901 1389933 33 +      
ENSG00000174672 E004 10.3323291 0.0016838146 2.571193e-25 2.889115e-23 11 1389934 1390201 268 + 1.473 0.238 -5.296
ENSG00000174672 E005 31.5496227 0.0038231644 6.831035e-13 1.722145e-11 11 1390202 1390284 83 + 1.747 1.252 -1.700
ENSG00000174672 E006 70.1650187 0.0171751159 5.281158e-04 2.264738e-03 11 1390285 1390375 91 + 1.978 1.718 -0.876
ENSG00000174672 E007 0.1817044 0.0393651673 8.144273e-01   11 1409400 1409631 232 + 0.000 0.095 8.262
ENSG00000174672 E008 0.1817044 0.0393651673 8.144273e-01   11 1409759 1409859 101 + 0.000 0.095 8.262
ENSG00000174672 E009 0.0000000       11 1410443 1410906 464 +      
ENSG00000174672 E010 0.0000000       11 1411152 1411633 482 +      
ENSG00000174672 E011 114.2077417 0.0066991126 1.678918e-03 6.215505e-03 11 1436040 1436134 95 + 2.132 1.966 -0.554
ENSG00000174672 E012 130.5196966 0.0039802680 1.115642e-02 3.154225e-02 11 1438306 1438391 86 + 2.160 2.043 -0.392
ENSG00000174672 E013 87.7575170 0.0046310794 2.435727e-03 8.569009e-03 11 1440788 1440798 11 + 2.013 1.855 -0.533
ENSG00000174672 E014 189.5555548 0.0027286112 2.049923e-02 5.254459e-02 11 1440799 1440928 130 + 2.301 2.214 -0.293
ENSG00000174672 E015 0.5212538 0.0212757527 9.300339e-01 9.599659e-01 11 1442445 1442489 45 + 0.157 0.172 0.164
ENSG00000174672 E016 203.6497061 0.0008386543 8.117801e-02 1.613806e-01 11 1442490 1442606 117 + 2.312 2.255 -0.190
ENSG00000174672 E017 122.7877690 0.0003329325 9.851226e-01 9.947102e-01 11 1443106 1443139 34 + 2.064 2.054 -0.031
ENSG00000174672 E018 161.9652071 0.0002350142 9.333325e-01 9.619817e-01 11 1443335 1443403 69 + 2.183 2.172 -0.038
ENSG00000174672 E019 184.0946011 0.0005224419 3.787616e-01 5.230585e-01 11 1443489 1443635 147 + 2.254 2.220 -0.112
ENSG00000174672 E020 82.3440082 0.0096097695 3.035006e-01 4.454184e-01 11 1444971 1445002 32 + 1.934 1.864 -0.235
ENSG00000174672 E021 154.4336892 0.0011088268 2.610085e-03 9.094928e-03 11 1445294 1445458 165 + 2.229 2.123 -0.355
ENSG00000174672 E022 127.6259011 0.0075622208 2.809456e-01 4.208081e-01 11 1445571 1445668 98 + 2.120 2.056 -0.213
ENSG00000174672 E023 151.1812582 0.0007435814 2.497589e-01 3.856398e-01 11 1445757 1445907 151 + 2.127 2.157 0.098
ENSG00000174672 E024 36.0278899 0.0006155370 2.676501e-02 6.550168e-02 11 1447798 1447863 66 + 1.440 1.572 0.452
ENSG00000174672 E025 97.1512310 0.0003824475 3.212476e-04 1.465891e-03 11 1449776 1449836 61 + 1.859 1.993 0.451
ENSG00000174672 E026 107.6859950 0.0003230181 4.404429e-03 1.427653e-02 11 1450587 1450657 71 + 1.933 2.030 0.325
ENSG00000174672 E027 98.9869540 0.0005272380 3.971139e-02 9.048839e-02 11 1450658 1450684 27 + 1.913 1.985 0.241
ENSG00000174672 E028 184.2595830 0.0002323509 8.743247e-02 1.712465e-01 11 1450685 1450794 110 + 2.203 2.243 0.131
ENSG00000174672 E029 165.3946737 0.0015604846 8.981945e-01 9.390431e-01 11 1451371 1451419 49 + 2.187 2.184 -0.007
ENSG00000174672 E030 28.6663525 0.0108236794 6.836734e-04 2.839289e-03 11 1453838 1454426 589 + 1.603 1.322 -0.969
ENSG00000174672 E031 13.9298551 0.0117834716 1.633887e-02 4.347279e-02 11 1454427 1454484 58 + 1.294 1.042 -0.899
ENSG00000174672 E032 265.0125400 0.0001657815 8.079000e-01 8.779368e-01 11 1454485 1454608 124 + 2.398 2.384 -0.048
ENSG00000174672 E033 346.2223842 0.0001515468 2.158478e-02 5.481905e-02 11 1456348 1456528 181 + 2.477 2.515 0.126
ENSG00000174672 E034 203.4569580 0.0001973601 1.028064e-02 2.945831e-02 11 1456598 1456687 90 + 2.232 2.292 0.199
ENSG00000174672 E035 51.7089145 0.0004777922 1.354791e-01 2.417841e-01 11 1456955 1457049 95 + 1.635 1.705 0.237
ENSG00000174672 E036 127.3131993 0.0003586109 4.351339e-01 5.773636e-01 11 1459192 1459236 45 + 2.057 2.075 0.059
ENSG00000174672 E037 78.4913290 0.0014082193 4.808519e-01 6.190687e-01 11 1459237 1459239 3 + 1.849 1.872 0.078
ENSG00000174672 E038 14.7043998 0.0012760969 5.156363e-05 2.895508e-04 11 1459240 1459447 208 + 1.373 1.011 -1.289
ENSG00000174672 E039 8.9262778 0.0705216314 3.227560e-01 4.660820e-01 11 1460500 1460547 48 + 1.059 0.904 -0.577
ENSG00000174672 E040 107.0957668 0.0045452836 7.643959e-01 8.474787e-01 11 1460548 1460969 422 + 1.991 2.000 0.030
ENSG00000174672 E041 633.2870265 0.0006113501 5.143245e-17 2.287259e-15 11 1460970 1462689 1720 + 2.665 2.809 0.478