ENSG00000174428

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000472837 ENSG00000174428 HEK293_OSMI2_2hA HEK293_TMG_2hB GTF2IRD2B protein_coding protein_coding 12.54633 17.63016 11.9097 1.447596 0.6140056 -0.5655157 1.025480 0.3970829 2.277922 0.09162185 0.3885875 2.4906436 0.1048667 0.0232000 0.1937667 0.17056667 2.937939e-06 1.158629e-14 FALSE TRUE
ENST00000614064 ENSG00000174428 HEK293_OSMI2_2hA HEK293_TMG_2hB GTF2IRD2B protein_coding protein_coding 12.54633 17.63016 11.9097 1.447596 0.6140056 -0.5655157 8.339322 13.9684531 5.718802 1.12496178 0.1763547 -1.2868993 0.6117458 0.7924667 0.4823333 -0.31013333 1.158629e-14 1.158629e-14 FALSE FALSE
MSTRG.30061.1 ENSG00000174428 HEK293_OSMI2_2hA HEK293_TMG_2hB GTF2IRD2B protein_coding   12.54633 17.63016 11.9097 1.447596 0.6140056 -0.5655157 1.842570 2.2041571 1.906610 0.30106546 0.5610754 -0.2082004 0.1587542 0.1239667 0.1572333 0.03326667 8.523503e-01 1.158629e-14 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000174428 E001 7.6737275 0.0023230293 3.901558e-01 5.341677e-01 7 75092573 75092589 17 + 0.886 0.987 0.381
ENSG00000174428 E002 8.3337121 0.0024105632 5.984365e-01 7.200325e-01 7 75092590 75092591 2 + 0.942 1.004 0.230
ENSG00000174428 E003 10.6879824 0.0016029418 5.822641e-01 7.066507e-01 7 75092592 75092599 8 + 1.037 1.096 0.214
ENSG00000174428 E004 16.7960637 0.0011485738 4.970368e-01 6.336020e-01 7 75092600 75092611 12 + 1.265 1.216 -0.171
ENSG00000174428 E005 32.0468399 0.0009337324 3.070176e-01 4.491880e-01 7 75092612 75092765 154 + 1.534 1.482 -0.179
ENSG00000174428 E006 0.8126314 0.0153787590 3.315304e-01 4.751682e-01 7 75093851 75095029 1179 + 0.316 0.145 -1.433
ENSG00000174428 E007 15.8502207 0.0047598658 3.491015e-02 8.148369e-02 7 75108960 75109063 104 + 1.289 1.111 -0.633
ENSG00000174428 E008 13.1851362 0.0014086856 8.793095e-01 9.264783e-01 7 75112397 75112535 139 + 1.141 1.160 0.068
ENSG00000174428 E009 5.0339640 0.0033596326 2.155677e-05 1.326189e-04 7 75112536 75112699 164 + 0.416 1.005 2.504
ENSG00000174428 E010 0.0000000       7 75120627 75120890 264 +      
ENSG00000174428 E011 11.1339047 0.0016123293 1.139564e-01 2.112831e-01 7 75120891 75121010 120 + 1.004 1.160 0.565
ENSG00000174428 E012 7.8030030 0.0023212167 4.156210e-02 9.392934e-02 7 75123136 75123319 184 + 0.821 1.053 0.873
ENSG00000174428 E013 0.2214452 0.0396032012 3.391703e-01   7 75123320 75123487 168 + 0.000 0.145 10.651
ENSG00000174428 E014 0.3697384 0.0257211835 1.156969e-01 2.138155e-01 7 75123488 75123516 29 + 0.000 0.253 11.614
ENSG00000174428 E015 1.1051668 0.1680729813 2.054091e-01 3.329267e-01 7 75123517 75123877 361 + 0.416 0.146 -2.002
ENSG00000174428 E016 0.0000000       7 75125657 75125915 259 +      
ENSG00000174428 E017 1.4069355 0.0785677648 2.514634e-01 3.877123e-01 7 75125916 75125970 55 + 0.256 0.476 1.312
ENSG00000174428 E018 0.5180316 0.0285977286 4.060443e-02 9.213322e-02 7 75126342 75126385 44 + 0.000 0.340 12.138
ENSG00000174428 E019 2.8647220 0.0056074644 6.575297e-06 4.555886e-05 7 75126386 75126495 110 + 0.103 0.841 4.472
ENSG00000174428 E020 0.0000000       7 75133135 75133154 20 +      
ENSG00000174428 E021 0.0000000       7 75133155 75133212 58 +      
ENSG00000174428 E022 0.0000000       7 75135001 75135057 57 +      
ENSG00000174428 E023 0.0000000       7 75136785 75136850 66 +      
ENSG00000174428 E024 0.0000000       7 75138950 75139027 78 +      
ENSG00000174428 E025 0.0000000       7 75141103 75141186 84 +      
ENSG00000174428 E026 0.4762024 0.1760897889 8.396902e-01 8.998625e-01 7 75142482 75142665 184 + 0.186 0.145 -0.431
ENSG00000174428 E027 0.9117205 0.0273125965 2.159383e-01 3.455316e-01 7 75143950 75143978 29 + 0.369 0.145 -1.756
ENSG00000174428 E028 1.1415848 0.0117333841 5.111060e-01 6.462146e-01 7 75143979 75143993 15 + 0.369 0.253 -0.755
ENSG00000174428 E029 0.0000000       7 75143994 75144068 75 +      
ENSG00000174428 E030 0.0000000       7 75146444 75146950 507 +      
ENSG00000174428 E031 34.3352453 0.0051611807 4.620017e-03 1.487604e-02 7 75147694 75149817 2124 + 1.617 1.451 -0.568