ENSG00000174165

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000310442 ENSG00000174165 HEK293_OSMI2_2hA HEK293_TMG_2hB ZDHHC24 protein_coding protein_coding 14.65677 25.74654 8.408847 1.595927 0.1871036 -1.613244 2.948009 4.381581 1.786804 0.3364989 0.17161082 -1.289307 0.2193250 0.1698667 0.2118333 0.04196667 0.32528630 0.001091158 FALSE TRUE
MSTRG.5779.4 ENSG00000174165 HEK293_OSMI2_2hA HEK293_TMG_2hB ZDHHC24 protein_coding   14.65677 25.74654 8.408847 1.595927 0.1871036 -1.613244 10.855837 20.583135 5.745823 1.1089352 0.09183158 -1.839069 0.7119792 0.8003333 0.6843667 -0.11596667 0.01000706 0.001091158 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000174165 E001 5.5283438 0.003281583 3.877449e-04 1.727346e-03 11 66520637 66521081 445 - 1.054 0.598 -1.803
ENSG00000174165 E002 7.2748443 0.002287069 1.277865e-06 1.037445e-05 11 66521082 66521466 385 - 1.209 0.652 -2.125
ENSG00000174165 E003 2.2990867 0.071695709 2.423118e-01 3.769013e-01 11 66524155 66524276 122 - 0.607 0.382 -1.110
ENSG00000174165 E004 1.6702255 0.009263400 5.434867e-02 1.168588e-01 11 66526936 66527006 71 - 0.606 0.272 -1.802
ENSG00000174165 E005 0.5503986 0.020371439 6.333276e-01 7.480502e-01 11 66529312 66529488 177 - 0.206 0.130 -0.800
ENSG00000174165 E006 33.5616025 0.002540316 2.257145e-03 8.021487e-03 11 66535626 66535828 203 - 1.209 1.481 0.945
ENSG00000174165 E007 164.8686063 0.001447199 7.619831e-02 1.534276e-01 11 66535829 66536867 1039 - 2.050 2.129 0.263
ENSG00000174165 E008 159.9491374 0.001360261 5.675132e-06 3.989080e-05 11 66536868 66538832 1965 - 2.213 2.066 -0.493
ENSG00000174165 E009 863.5011449 0.003046505 3.462293e-03 1.161765e-02 11 66538833 66539824 992 - 2.774 2.845 0.235
ENSG00000174165 E010 2.5390821 0.006130981 3.626870e-02 8.409929e-02 11 66542484 66542828 345 - 0.721 0.379 -1.609
ENSG00000174165 E011 437.6192519 0.001022394 7.322901e-01 8.237791e-01 11 66543704 66543981 278 - 2.518 2.538 0.065
ENSG00000174165 E012 253.9875321 0.007165059 2.483521e-02 6.153728e-02 11 66545723 66546235 513 - 2.365 2.283 -0.271