ENSG00000173744

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000310078 ENSG00000173744 HEK293_OSMI2_2hA HEK293_TMG_2hB AGFG1 protein_coding protein_coding 14.6567 7.868037 21.8882 0.3023146 0.3551508 1.474904 5.277256 3.6035772 8.095989 0.5360090 0.3709671 1.1655605 0.36841667 0.4605333 0.37040000 -0.09013333 6.139753e-01 2.466974e-19 FALSE TRUE
ENST00000409171 ENSG00000173744 HEK293_OSMI2_2hA HEK293_TMG_2hB AGFG1 protein_coding protein_coding 14.6567 7.868037 21.8882 0.3023146 0.3551508 1.474904 1.259132 0.8174469 1.765165 0.1529788 0.2701235 1.1012148 0.08503333 0.1054667 0.08026667 -0.02520000 7.179753e-01 2.466974e-19 FALSE TRUE
ENST00000456594 ENSG00000173744 HEK293_OSMI2_2hA HEK293_TMG_2hB AGFG1 protein_coding protein_coding 14.6567 7.868037 21.8882 0.3023146 0.3551508 1.474904 5.227432 3.4470124 6.744328 0.7297298 0.1211136 0.9662866 0.38070833 0.4340000 0.30836667 -0.12563333 4.295070e-01 2.466974e-19 FALSE FALSE
ENST00000486932 ENSG00000173744 HEK293_OSMI2_2hA HEK293_TMG_2hB AGFG1 protein_coding processed_transcript 14.6567 7.868037 21.8882 0.3023146 0.3551508 1.474904 2.396889 0.0000000 4.967003 0.0000000 0.6833317 8.9591336 0.12352917 0.0000000 0.22646667 0.22646667 2.466974e-19 2.466974e-19   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000173744 E001 0.1515154 0.0426859790 8.882470e-01   2 227472132 227472151 20 + 0.096 0.000 -10.888
ENSG00000173744 E002 0.1515154 0.0426859790 8.882470e-01   2 227472152 227472155 4 + 0.096 0.000 -10.884
ENSG00000173744 E003 18.0647984 0.0213792217 1.039119e-06 8.590794e-06 2 227472156 227472368 213 + 1.438 0.817 -2.249
ENSG00000173744 E004 7.4342740 0.0363447530 2.425063e-03 8.537061e-03 2 227472369 227472371 3 + 1.066 0.553 -2.050
ENSG00000173744 E005 8.9599802 0.0309075297 1.368831e-02 3.751144e-02 2 227472372 227472384 13 + 1.109 0.724 -1.463
ENSG00000173744 E006 84.8198390 0.0003104941 6.019531e-08 6.379760e-07 2 227472385 227472588 204 + 2.011 1.769 -0.814
ENSG00000173744 E007 109.0335333 0.0002983475 1.269487e-03 4.875538e-03 2 227491547 227491640 94 + 2.085 1.952 -0.445
ENSG00000173744 E008 0.1515154 0.0426859790 8.882470e-01   2 227508272 227508650 379 + 0.096 0.000 -10.884
ENSG00000173744 E009 0.0000000       2 227510721 227510864 144 +      
ENSG00000173744 E010 113.2835575 0.0006666874 4.448135e-02 9.925579e-02 2 227519948 227520045 98 + 2.087 1.996 -0.306
ENSG00000173744 E011 80.8972318 0.0003296840 1.757629e-01 2.956830e-01 2 227520046 227520063 18 + 1.936 1.863 -0.246
ENSG00000173744 E012 188.2627708 0.0035647622 1.814452e-01 3.030598e-01 2 227523763 227523925 163 + 2.299 2.224 -0.251
ENSG00000173744 E013 185.8903821 0.0102999167 7.963396e-01 8.700004e-01 2 227524762 227524915 154 + 2.280 2.239 -0.137
ENSG00000173744 E014 157.1700999 0.0011472814 3.713706e-01 5.158070e-01 2 227531091 227531210 120 + 2.188 2.196 0.027
ENSG00000173744 E015 0.2966881 0.0290785164 4.236378e-01   2 227532138 227532209 72 + 0.175 0.000 -11.884
ENSG00000173744 E016 222.7851050 0.0002934839 9.332792e-01 9.619645e-01 2 227533549 227533758 210 + 2.349 2.333 -0.056
ENSG00000173744 E017 134.9349230 0.0003013349 6.130759e-03 1.895261e-02 2 227534845 227534907 63 + 2.098 2.165 0.222
ENSG00000173744 E018 157.9816332 0.0003642112 3.473855e-03 1.164984e-02 2 227534908 227535025 118 + 2.169 2.235 0.222
ENSG00000173744 E019 133.5809126 0.0002769621 5.377468e-04 2.301209e-03 2 227536625 227536704 80 + 2.087 2.176 0.297
ENSG00000173744 E020 124.7602054 0.0002568091 1.415925e-02 3.858720e-02 2 227536901 227536954 54 + 2.070 2.130 0.202
ENSG00000173744 E021 124.8352651 0.0003280288 1.325974e-01 2.377026e-01 2 227536955 227536993 39 + 2.082 2.112 0.101
ENSG00000173744 E022 0.3030308 0.3133249867 5.158091e-01   2 227550059 227550106 48 + 0.175 0.000 -11.890
ENSG00000173744 E023 196.9740745 0.0002017647 8.494719e-05 4.514542e-04 2 227551959 227552117 159 + 2.257 2.338 0.271
ENSG00000173744 E024 68.2027093 0.0003609219 1.166414e-03 4.526755e-03 2 227553704 227553709 6 + 1.781 1.902 0.408
ENSG00000173744 E025 142.3418551 0.0002162647 7.451913e-05 4.018738e-04 2 227553710 227553795 86 + 2.108 2.207 0.333
ENSG00000173744 E026 895.5835559 0.0090814358 1.395390e-01 2.473889e-01 2 227554436 227561217 6782 + 2.973 2.905 -0.229