ENSG00000173327

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000309100 ENSG00000173327 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP3K11 protein_coding protein_coding 23.177 43.51407 14.31641 1.846129 0.3855874 -1.603136 15.296854 28.873397 8.93626648 2.6630662 1.25981782 -1.6908828 0.64499167 0.6609 0.620433333 -0.04046667 0.882137804 0.004090028 FALSE TRUE
ENST00000524856 ENSG00000173327 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP3K11 protein_coding retained_intron 23.177 43.51407 14.31641 1.846129 0.3855874 -1.603136 3.150435 4.768989 2.89376756 0.6764806 0.18429743 -0.7187795 0.15557500 0.1087 0.202266667 0.09356667 0.011458450 0.004090028 TRUE TRUE
ENST00000532507 ENSG00000173327 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP3K11 protein_coding protein_coding 23.177 43.51407 14.31641 1.846129 0.3855874 -1.603136 1.983513 6.241410 0.06048492 2.2421569 0.06048492 -6.4707233 0.05827917 0.1471 0.004066667 -0.14303333 0.004090028 0.004090028 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000173327 E001 3.5619979 0.0984284444 1.214302e-02 3.387511e-02 11 65597756 65597756 1 - 0.904 0.427 -2.069
ENSG00000173327 E002 5.4033161 0.0741695849 8.661952e-02 1.699899e-01 11 65597757 65597757 1 - 0.934 0.638 -1.183
ENSG00000173327 E003 290.5097033 0.0022287907 1.316805e-06 1.065679e-05 11 65597758 65598019 262 - 2.226 2.401 0.586
ENSG00000173327 E004 770.4528206 0.0040224468 5.678637e-05 3.155304e-04 11 65598020 65598574 555 - 2.683 2.819 0.453
ENSG00000173327 E005 155.9222459 0.0005099780 1.100540e-02 3.117480e-02 11 65598575 65598628 54 - 2.022 2.119 0.325
ENSG00000173327 E006 136.5814919 0.0002420256 1.701049e-01 2.883230e-01 11 65599394 65599450 57 - 1.998 2.053 0.184
ENSG00000173327 E007 235.6219865 0.0001884862 5.554857e-01 6.843362e-01 11 65599451 65599768 318 - 2.260 2.281 0.068
ENSG00000173327 E008 1.5049910 0.0111686796 4.319987e-01 5.744790e-01 11 65605593 65605642 50 - 0.199 0.357 1.130
ENSG00000173327 E009 1.3932309 0.0098925567 9.294676e-01 9.595995e-01 11 65605722 65605760 39 - 0.335 0.320 -0.094
ENSG00000173327 E010 182.8043523 0.0002052812 6.929214e-02 1.421892e-01 11 65605761 65605852 92 - 2.117 2.180 0.210
ENSG00000173327 E011 229.4062967 0.0002206699 4.534637e-02 1.008278e-01 11 65605946 65606081 136 - 2.215 2.277 0.207
ENSG00000173327 E012 0.5159433 0.2213310650 1.650370e-01 2.817447e-01 11 65606082 65606396 315 - 0.335 0.074 -2.658
ENSG00000173327 E013 1.2575084 0.1217402697 1.383910e-01 2.458506e-01 11 65606435 65606690 256 - 0.000 0.356 10.322
ENSG00000173327 E014 186.8681845 0.0019326032 4.806651e-01 6.188996e-01 11 65606691 65606804 114 - 2.191 2.172 -0.065
ENSG00000173327 E015 0.6255398 0.0195414649 9.600643e-01 9.789089e-01 11 65606805 65607026 222 - 0.199 0.189 -0.098
ENSG00000173327 E016 0.5085815 0.2350472526 6.951894e-01 7.958231e-01 11 65607165 65607269 105 - 0.199 0.135 -0.671
ENSG00000173327 E017 222.4782843 0.0003306361 3.560161e-02 8.281296e-02 11 65607270 65607513 244 - 2.295 2.240 -0.186
ENSG00000173327 E018 159.2149139 0.0002116587 7.620771e-01 8.457667e-01 11 65607641 65607697 57 - 2.097 2.111 0.047
ENSG00000173327 E019 110.9794852 0.0002593553 9.840146e-01 9.940860e-01 11 65607698 65607705 8 - 1.949 1.952 0.010
ENSG00000173327 E020 220.0071801 0.0001901434 8.792959e-01 9.264737e-01 11 65607706 65607801 96 - 2.242 2.250 0.028
ENSG00000173327 E021 140.6129682 0.0002797317 7.725483e-01 8.531863e-01 11 65607802 65607816 15 - 2.046 2.060 0.048
ENSG00000173327 E022 8.9044887 0.0097265349 8.849674e-01 9.304726e-01 11 65607817 65607921 105 - 0.903 0.926 0.088
ENSG00000173327 E023 135.5687944 0.0011545397 7.379854e-01 8.279931e-01 11 65607922 65607929 8 - 2.027 2.045 0.060
ENSG00000173327 E024 224.0326333 0.0002418381 7.405537e-01 8.299456e-01 11 65607930 65608023 94 - 2.262 2.256 -0.018
ENSG00000173327 E025 177.5241197 0.0002095713 3.650728e-02 8.454695e-02 11 65608024 65608070 47 - 2.203 2.142 -0.204
ENSG00000173327 E026 4.7512726 0.0110178869 5.954901e-02 1.258465e-01 11 65608071 65608267 197 - 0.870 0.594 -1.133
ENSG00000173327 E027 310.3681048 0.0001795076 9.369760e-02 1.808992e-01 11 65608268 65608434 167 - 2.425 2.389 -0.120
ENSG00000173327 E028 131.3784857 0.0024066528 1.323635e-01 2.373890e-01 11 65608435 65608448 14 - 2.070 2.011 -0.197
ENSG00000173327 E029 15.0138561 0.0056940610 5.948778e-01 7.170475e-01 11 65608449 65608548 100 - 1.158 1.107 -0.183
ENSG00000173327 E030 4.6811513 0.0036270517 8.642946e-01 9.165199e-01 11 65608549 65608631 83 - 0.653 0.683 0.127
ENSG00000173327 E031 34.9785549 0.0006245841 5.968747e-01 7.187286e-01 11 65608632 65610811 2180 - 1.487 1.454 -0.113
ENSG00000173327 E032 29.6775775 0.0061016777 4.358532e-04 1.912297e-03 11 65610812 65613017 2206 - 1.589 1.324 -0.915
ENSG00000173327 E033 732.0220169 0.0015547079 4.637394e-01 6.035092e-01 11 65613018 65614009 992 - 2.777 2.768 -0.029
ENSG00000173327 E034 224.8528082 0.0093190777 2.496201e-01 3.854694e-01 11 65614010 65614317 308 - 2.299 2.249 -0.166
ENSG00000173327 E035 207.8477927 0.0064024873 2.380892e-07 2.248855e-06 11 65614318 65615119 802 - 2.396 2.167 -0.767
ENSG00000173327 E036 81.7588459 0.0020079344 1.318921e-11 2.693968e-10 11 65615120 65615382 263 - 2.046 1.739 -1.034