ENSG00000173226

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000310864 ENSG00000173226 HEK293_OSMI2_2hA HEK293_TMG_2hB IQCB1 protein_coding protein_coding 26.67198 12.52559 45.51177 1.410362 1.843919 1.860527 6.552804 5.2532713 9.501158 1.4301049 0.2749754 0.8536613 0.28082083 0.42243333 0.20920000 -0.213233333 0.2088312979 0.0006478047 FALSE TRUE
ENST00000349820 ENSG00000173226 HEK293_OSMI2_2hA HEK293_TMG_2hB IQCB1 protein_coding protein_coding 26.67198 12.52559 45.51177 1.410362 1.843919 1.860527 3.307491 1.6778120 5.564861 0.3274251 0.3598032 1.7237818 0.12762500 0.13426667 0.12310000 -0.011166667 0.9063714971 0.0006478047 FALSE TRUE
ENST00000393650 ENSG00000173226 HEK293_OSMI2_2hA HEK293_TMG_2hB IQCB1 protein_coding nonsense_mediated_decay 26.67198 12.52559 45.51177 1.410362 1.843919 1.860527 1.635079 0.7909113 2.531723 0.4005226 0.1178686 1.6660925 0.05408333 0.06383333 0.05596667 -0.007866667 0.9171729777 0.0006478047 TRUE TRUE
ENST00000471726 ENSG00000173226 HEK293_OSMI2_2hA HEK293_TMG_2hB IQCB1 protein_coding retained_intron 26.67198 12.52559 45.51177 1.410362 1.843919 1.860527 2.320123 0.4154361 4.290207 0.1151941 0.3808796 3.3373918 0.08153333 0.03233333 0.09483333 0.062500000 0.0006478047 0.0006478047   FALSE
MSTRG.23530.4 ENSG00000173226 HEK293_OSMI2_2hA HEK293_TMG_2hB IQCB1 protein_coding   26.67198 12.52559 45.51177 1.410362 1.843919 1.860527 3.212617 0.9814817 4.911672 0.3750192 0.8198027 2.3114904 0.12274167 0.08170000 0.10833333 0.026633333 0.7231375229 0.0006478047 TRUE TRUE
MSTRG.23530.6 ENSG00000173226 HEK293_OSMI2_2hA HEK293_TMG_2hB IQCB1 protein_coding   26.67198 12.52559 45.51177 1.410362 1.843919 1.860527 1.693333 1.4856006 4.158473 0.4890895 1.1046548 1.4787942 0.07008750 0.11323333 0.09360000 -0.019633333 0.8218458118 0.0006478047 FALSE TRUE
MSTRG.23530.9 ENSG00000173226 HEK293_OSMI2_2hA HEK293_TMG_2hB IQCB1 protein_coding   26.67198 12.52559 45.51177 1.410362 1.843919 1.860527 5.714788 1.7077770 10.552204 0.8833317 1.6428182 2.6202962 0.19810417 0.13626667 0.22970000 0.093433333 0.7251434405 0.0006478047 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000173226 E001 32.8421617 0.0305068187 3.732727e-03 1.239099e-02 3 121769761 121769993 233 - 1.362 1.711 1.196
ENSG00000173226 E002 64.3587059 0.0086834837 3.784363e-08 4.177950e-07 3 121769994 121770098 105 - 1.638 2.003 1.233
ENSG00000173226 E003 65.6863436 0.0109968780 1.167201e-05 7.637301e-05 3 121770099 121770138 40 - 1.671 1.988 1.071
ENSG00000173226 E004 117.9160168 0.0085810962 5.526777e-07 4.849163e-06 3 121770139 121770212 74 - 1.928 2.227 1.003
ENSG00000173226 E005 94.7065146 0.0029112781 4.139993e-10 6.584179e-09 3 121770213 121770217 5 - 1.835 2.132 0.995
ENSG00000173226 E006 92.7784151 0.0012575273 7.286048e-11 1.315467e-09 3 121770218 121770219 2 - 1.835 2.114 0.935
ENSG00000173226 E007 333.3659233 0.0008398162 2.414700e-11 4.727267e-10 3 121770220 121770574 355 - 2.431 2.600 0.561
ENSG00000173226 E008 217.7536941 0.0001668754 5.897043e-02 1.248717e-01 3 121772557 121772713 157 - 2.287 2.350 0.208
ENSG00000173226 E009 229.0186352 0.0001883986 5.896278e-02 1.248583e-01 3 121781743 121781874 132 - 2.341 2.308 -0.111
ENSG00000173226 E010 216.6697948 0.0001798291 1.941643e-02 5.023673e-02 3 121788284 121788432 149 - 2.320 2.274 -0.155
ENSG00000173226 E011 231.5128876 0.0027351425 1.790851e-01 2.999933e-01 3 121790073 121790215 143 - 2.343 2.308 -0.117
ENSG00000173226 E012 88.9202058 0.0003411811 3.642866e-02 8.440099e-02 3 121795457 121795460 4 - 1.943 1.870 -0.246
ENSG00000173226 E013 160.4644629 0.0004617491 3.115069e-02 7.419417e-02 3 121795461 121795566 106 - 2.192 2.137 -0.183
ENSG00000173226 E014 127.5242864 0.0020267108 1.546596e-01 2.679591e-01 3 121797118 121797187 70 - 2.089 2.043 -0.154
ENSG00000173226 E015 106.8213079 0.0003114519 6.112545e-01 7.303803e-01 3 121797188 121797227 40 - 2.000 1.995 -0.016
ENSG00000173226 E016 177.2395081 0.0029784856 1.398228e-02 3.818510e-02 3 121799196 121799374 179 - 2.241 2.157 -0.281
ENSG00000173226 E017 142.3008502 0.0002347813 6.396076e-05 3.508563e-04 3 121807344 121807443 100 - 2.157 2.037 -0.399
ENSG00000173226 E018 106.4241595 0.0003078201 1.090053e-04 5.634732e-04 3 121808916 121809009 94 - 2.036 1.900 -0.458
ENSG00000173226 E019 0.6266857 0.0169822963 3.598176e-01 5.041304e-01 3 121811816 121811865 50 - 0.146 0.313 1.402
ENSG00000173226 E020 139.5980799 0.0003116607 3.385253e-04 1.534713e-03 3 121826051 121826180 130 - 2.146 2.039 -0.358
ENSG00000173226 E021 96.9300714 0.0051695010 5.080451e-03 1.615021e-02 3 121828470 121828532 63 - 1.998 1.857 -0.475
ENSG00000173226 E022 105.2468128 0.0112809416 2.743085e-02 6.686773e-02 3 121828533 121828632 100 - 2.033 1.897 -0.457
ENSG00000173226 E023 117.5524400 0.0002867034 2.993634e-08 3.369785e-07 3 121828861 121828972 112 - 2.095 1.899 -0.656
ENSG00000173226 E024 24.6840943 0.0093219296 5.041227e-06 3.585139e-05 3 121833936 121834248 313 - 1.474 1.023 -1.593
ENSG00000173226 E025 14.9418722 0.0012050490 6.004293e-05 3.316603e-04 3 121834249 121834390 142 - 1.265 0.834 -1.579
ENSG00000173226 E026 88.6313713 0.0009966949 1.568078e-05 9.963701e-05 3 121834391 121834479 89 - 1.969 1.788 -0.612
ENSG00000173226 E027 56.9151097 0.0004731777 2.801755e-04 1.299095e-03 3 121834966 121835079 114 - 1.780 1.598 -0.616