ENSG00000173166

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000308091 ENSG00000173166 HEK293_OSMI2_2hA HEK293_TMG_2hB RAPH1 protein_coding protein_coding 2.640271 1.865384 3.388621 0.1338607 0.1354897 0.8577633 0.2044374 0.02695399 0.5054956 0.013711 0.06630875 3.8021581 0.07007917 0.01546667 0.14810000 0.13263333 2.681378e-04 1.459939e-06 FALSE TRUE
ENST00000319170 ENSG00000173166 HEK293_OSMI2_2hA HEK293_TMG_2hB RAPH1 protein_coding protein_coding 2.640271 1.865384 3.388621 0.1338607 0.1354897 0.8577633 2.2015833 1.82246826 2.4230699 0.148071 0.14109618 0.4089896 0.85529167 0.97556667 0.71553333 -0.26003333 1.459939e-06 1.459939e-06 FALSE TRUE
ENST00000439222 ENSG00000173166 HEK293_OSMI2_2hA HEK293_TMG_2hB RAPH1 protein_coding protein_coding 2.640271 1.865384 3.388621 0.1338607 0.1354897 0.8577633 0.1385754 0.00000000 0.2653502 0.000000 0.17030767 4.7831960 0.04301667 0.00000000 0.08146667 0.08146667 3.376881e-01 1.459939e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000173166 E001 0.1515154 0.042482839 7.530300e-01   2 203394345 203395089 745 - 0.077 0.000 -10.220
ENSG00000173166 E002 416.5700634 0.012930755 2.204727e-21 1.652865e-19 2 203433682 203439436 5755 - 2.374 2.858 1.613
ENSG00000173166 E003 70.6447821 0.006330087 4.153084e-03 1.357235e-02 2 203439437 203441413 1977 - 1.792 1.867 0.255
ENSG00000173166 E004 5.3972125 0.003823415 1.490868e-03 5.606870e-03 2 203441470 203442077 608 - 0.848 0.570 -1.157
ENSG00000173166 E005 0.7687379 0.073836474 4.167956e-01 5.601716e-01 2 203442078 203442080 3 - 0.250 0.188 -0.521
ENSG00000173166 E006 0.5117906 0.193182869 9.188853e-01 9.527633e-01 2 203444631 203444849 219 - 0.143 0.190 0.499
ENSG00000173166 E007 0.2966881 0.027442404 3.652229e-01   2 203444850 203444867 18 - 0.143 0.000 -13.258
ENSG00000173166 E008 14.4940038 0.001301155 6.429029e-03 1.973716e-02 2 203444868 203445010 143 - 1.166 1.130 -0.129
ENSG00000173166 E009 17.7549375 0.001090747 2.174710e-03 7.764404e-03 2 203447959 203448079 121 - 1.250 1.209 -0.143
ENSG00000173166 E010 14.7327291 0.001203768 6.801205e-04 2.826517e-03 2 203448738 203448836 99 - 1.194 1.093 -0.360
ENSG00000173166 E011 14.7466571 0.001186117 1.004894e-02 2.888220e-02 2 203454430 203454540 111 - 1.166 1.147 -0.068
ENSG00000173166 E012 15.1712164 0.005711854 6.186296e-04 2.601849e-03 2 203455437 203455580 144 - 1.200 1.075 -0.446
ENSG00000173166 E013 12.4080593 0.127124916 6.375756e-03 1.959965e-02 2 203457530 203457595 66 - 1.161 0.880 -1.033
ENSG00000173166 E014 18.4024553 0.003280116 3.074114e-08 3.452378e-07 2 203459907 203460028 122 - 1.320 1.011 -1.106
ENSG00000173166 E015 16.8641689 0.093411904 3.676374e-02 8.501907e-02 2 203461249 203461408 160 - 1.248 1.124 -0.441
ENSG00000173166 E016 9.3201626 0.013980133 2.080766e-01 3.361701e-01 2 203461848 203461925 78 - 0.954 1.012 0.215
ENSG00000173166 E017 2.5359536 0.007752958 2.145240e-03 7.675921e-03 2 203470239 203470313 75 - 0.615 0.188 -2.523
ENSG00000173166 E018 2.5057767 0.017034969 1.878200e-02 4.885750e-02 2 203477076 203477156 81 - 0.594 0.319 -1.433
ENSG00000173166 E019 16.1119604 0.001081942 2.696527e-07 2.519228e-06 2 203489584 203489799 216 - 1.264 0.986 -0.999
ENSG00000173166 E020 10.3868557 0.001658499 1.720778e-07 1.670491e-06 2 203489800 203489890 91 - 1.117 0.681 -1.671
ENSG00000173166 E021 13.0839864 0.001356358 2.742511e-07 2.557773e-06 2 203489891 203489993 103 - 1.189 0.843 -1.273
ENSG00000173166 E022 10.5698012 0.001845912 3.633375e-04 1.632086e-03 2 203489994 203490045 52 - 1.069 0.876 -0.719
ENSG00000173166 E023 12.3568242 0.003764652 4.440128e-05 2.533336e-04 2 203490046 203490086 41 - 1.142 0.907 -0.865
ENSG00000173166 E024 7.8067275 0.018656491 2.193247e-03 7.821106e-03 2 203490087 203490089 3 - 0.963 0.730 -0.906
ENSG00000173166 E025 13.9033348 0.001323179 8.437977e-05 4.487786e-04 2 203491214 203491319 106 - 1.183 1.010 -0.626
ENSG00000173166 E026 0.0000000       2 203495163 203495233 71 -      
ENSG00000173166 E027 11.6262936 0.010388059 2.325131e-04 1.102077e-03 2 203495234 203495353 120 - 1.124 0.906 -0.800
ENSG00000173166 E028 0.0000000       2 203533365 203533433 69 -      
ENSG00000173166 E029 7.3740033 0.022987721 3.971089e-03 1.306073e-02 2 203535111 203535335 225 - 0.945 0.728 -0.842