ENSG00000172671

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000344646 ENSG00000172671 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFAND4 protein_coding protein_coding 5.067562 2.529166 6.568717 0.1742625 0.2056393 1.373452 0.92891014 0.7841856 1.4258264 0.02919019 0.14682657 0.8543332 0.20397083 0.31430000 0.2188000 -0.09550000 0.3450577403 0.0002483447 FALSE TRUE
ENST00000374370 ENSG00000172671 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFAND4 protein_coding processed_transcript 5.067562 2.529166 6.568717 0.1742625 0.2056393 1.373452 0.05953924 0.1845761 0.0000000 0.18457605 0.00000000 -4.2822623 0.02410000 0.07000000 0.0000000 -0.07000000 0.6340673771 0.0002483447 FALSE TRUE
ENST00000484333 ENSG00000172671 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFAND4 protein_coding processed_transcript 5.067562 2.529166 6.568717 0.1742625 0.2056393 1.373452 0.93115076 0.6263716 1.0107396 0.12162601 0.08302606 0.6816735 0.20710833 0.24753333 0.1542667 -0.09326667 0.2093164604 0.0002483447   FALSE
ENST00000497028 ENSG00000172671 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFAND4 protein_coding processed_transcript 5.067562 2.529166 6.568717 0.1742625 0.2056393 1.373452 0.57248216 0.1502088 0.8171788 0.08977784 0.36783090 2.3682462 0.09902917 0.05613333 0.1213667 0.06523333 0.6103965301 0.0002483447   FALSE
MSTRG.3885.10 ENSG00000172671 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFAND4 protein_coding   5.067562 2.529166 6.568717 0.1742625 0.2056393 1.373452 0.27479009 0.0000000 0.8919892 0.00000000 0.35260592 6.4950383 0.04555417 0.00000000 0.1329333 0.13293333 0.0002483447 0.0002483447 FALSE TRUE
MSTRG.3885.13 ENSG00000172671 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFAND4 protein_coding   5.067562 2.529166 6.568717 0.1742625 0.2056393 1.373452 0.59143611 0.1129535 0.8968734 0.11295349 0.03514892 2.8827886 0.12277500 0.05163333 0.1371333 0.08550000 0.2759476296 0.0002483447 TRUE TRUE
MSTRG.3885.5 ENSG00000172671 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFAND4 protein_coding   5.067562 2.529166 6.568717 0.1742625 0.2056393 1.373452 0.46183283 0.1855082 0.0000000 0.18550823 0.00000000 -4.2891575 0.08110417 0.06716667 0.0000000 -0.06716667 0.5818873929 0.0002483447 FALSE TRUE
MSTRG.3885.7 ENSG00000172671 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFAND4 protein_coding   5.067562 2.529166 6.568717 0.1742625 0.2056393 1.373452 0.55828139 0.0000000 0.6490974 0.00000000 0.33033329 6.0424199 0.09280417 0.00000000 0.1020667 0.10206667 0.3259763267 0.0002483447 FALSE TRUE
MSTRG.3885.8 ENSG00000172671 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFAND4 protein_coding   5.067562 2.529166 6.568717 0.1742625 0.2056393 1.373452 0.21247875 0.2121639 0.0000000 0.12980923 0.00000000 -4.4735526 0.03277500 0.07936667 0.0000000 -0.07936667 0.0433863950 0.0002483447 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000172671 E001 0.4448795 0.3847858413 7.093588e-02 1.448920e-01 10 45615500 45615500 1 - 0.000 0.376 11.683
ENSG00000172671 E002 0.4448795 0.3847858413 7.093588e-02 1.448920e-01 10 45615501 45615598 98 - 0.000 0.376 13.972
ENSG00000172671 E003 23.5571617 0.0009218863 2.730481e-04 1.269994e-03 10 45615599 45616262 664 - 1.258 1.517 0.897
ENSG00000172671 E004 9.9976199 0.0017192279 5.819663e-03 1.813626e-02 10 45616263 45616382 120 - 0.904 1.190 1.044
ENSG00000172671 E005 3.3547348 0.0048268610 2.790553e-01 4.187494e-01 10 45616383 45616384 2 - 0.560 0.728 0.722
ENSG00000172671 E006 3.3547348 0.0048268610 2.790553e-01 4.187494e-01 10 45616385 45616385 1 - 0.560 0.728 0.722
ENSG00000172671 E007 3.7276954 0.0042223374 2.319115e-01 3.647324e-01 10 45616386 45616388 3 - 0.586 0.765 0.759
ENSG00000172671 E008 3.8728681 0.0042129486 2.980650e-01 4.394599e-01 10 45616389 45616390 2 - 0.610 0.765 0.652
ENSG00000172671 E009 5.6693373 0.0033229472 6.005184e-02 1.266886e-01 10 45616391 45616404 14 - 0.695 0.941 0.969
ENSG00000172671 E010 9.3428957 0.0019501411 2.657145e-02 6.509947e-02 10 45616405 45616486 82 - 0.892 1.132 0.884
ENSG00000172671 E011 8.9827238 0.0019759514 2.199723e-01 3.503791e-01 10 45616487 45616571 85 - 0.927 1.065 0.509
ENSG00000172671 E012 10.5618408 0.0017026020 1.302460e-01 2.345060e-01 10 45617765 45618139 375 - 0.990 1.147 0.569
ENSG00000172671 E013 16.1203154 0.0077333236 6.427022e-01 7.554397e-01 10 45618140 45618260 121 - 1.202 1.252 0.176
ENSG00000172671 E014 3.0558937 0.0068295809 1.191086e-03 4.611079e-03 10 45620780 45620865 86 - 0.365 0.861 2.250
ENSG00000172671 E015 2.0262724 0.1535707123 4.811722e-02 1.058361e-01 10 45620866 45620927 62 - 0.274 0.687 2.136
ENSG00000172671 E016 9.0133797 0.0295822909 4.115304e-01 5.550878e-01 10 45624583 45624637 55 - 0.949 1.061 0.415
ENSG00000172671 E017 31.0791082 0.0009326901 1.129002e-01 2.097745e-01 10 45625951 45626257 307 - 1.450 1.553 0.353
ENSG00000172671 E018 37.0213197 0.0006732822 1.947229e-01 3.196513e-01 10 45626258 45626570 313 - 1.530 1.608 0.266
ENSG00000172671 E019 49.7041858 0.0053544773 8.060263e-01 8.766894e-01 10 45626571 45627105 535 - 1.693 1.682 -0.035
ENSG00000172671 E020 3.2964452 0.0054257543 2.832347e-01 4.233270e-01 10 45639706 45639815 110 - 0.560 0.727 0.720
ENSG00000172671 E021 25.6106750 0.0008571358 3.636678e-01 5.080898e-01 10 45639816 45639963 148 - 1.426 1.366 -0.210
ENSG00000172671 E022 0.9545818 0.0140410915 9.222893e-01 9.549787e-01 10 45640241 45640421 181 - 0.274 0.293 0.138
ENSG00000172671 E023 27.9929254 0.0008021709 2.646430e-02 6.487721e-02 10 45648294 45648418 125 - 1.489 1.338 -0.524
ENSG00000172671 E024 21.0531685 0.0009813560 1.205572e-01 2.207624e-01 10 45648419 45648474 56 - 1.361 1.241 -0.419
ENSG00000172671 E025 22.9191431 0.0023835682 1.435160e-01 2.528026e-01 10 45648475 45648534 60 - 1.396 1.286 -0.383
ENSG00000172671 E026 3.1627385 0.0086019592 2.637418e-01 4.016487e-01 10 45648963 45649104 142 - 0.654 0.467 -0.860
ENSG00000172671 E027 0.1482932 0.0411772654 2.238476e-01   10 45649732 45649779 48 - 0.000 0.171 12.787
ENSG00000172671 E028 1.6952202 0.0083114980 3.250585e-01 4.684917e-01 10 45650125 45651625 1501 - 0.473 0.293 -1.032
ENSG00000172671 E029 20.6869758 0.0012150213 1.927440e-01 3.172136e-01 10 45651966 45652033 68 - 1.352 1.253 -0.348
ENSG00000172671 E030 15.5275999 0.0012768136 3.658323e-01 5.101381e-01 10 45652984 45653059 76 - 1.225 1.147 -0.278
ENSG00000172671 E031 0.2924217 0.0271968184 5.138505e-01   10 45662580 45662681 102 - 0.158 0.000 -11.947
ENSG00000172671 E032 36.2657125 0.0006414430 1.797557e-03 6.591726e-03 10 45663542 45663842 301 - 1.607 1.417 -0.654
ENSG00000172671 E033 4.0759852 0.0046267612 1.346831e-02 3.700766e-02 10 45672250 45672271 22 - 0.797 0.389 -1.863
ENSG00000172671 E034 23.2752413 0.0008050453 2.944756e-06 2.207221e-05 10 45672272 45672678 407 - 1.467 1.083 -1.350
ENSG00000172671 E035 4.0085696 0.0128462494 1.601874e-02 4.277604e-02 10 45672679 45672780 102 - 0.797 0.388 -1.864