ENSG00000172458

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000304920 ENSG00000172458 HEK293_OSMI2_2hA HEK293_TMG_2hB IL17D protein_coding protein_coding 1.729635 2.418718 1.613077 0.0522236 0.1602556 -0.581464 1.1769793 2.25706184 0.9244382 0.04549167 0.4776438 -1.278653 0.6949167 0.93440000 0.5216000 -0.4128000 0.36909394 0.03925679 FALSE TRUE
ENST00000468605 ENSG00000172458 HEK293_OSMI2_2hA HEK293_TMG_2hB IL17D protein_coding nonsense_mediated_decay 1.729635 2.418718 1.613077 0.0522236 0.1602556 -0.581464 0.1534988 0.07016426 0.1799798 0.03510833 0.1799798 1.244815 0.1265042 0.02870000 0.1370333 0.1083333 0.93838831 0.03925679 TRUE FALSE
ENST00000682841 ENSG00000172458 HEK293_OSMI2_2hA HEK293_TMG_2hB IL17D protein_coding protein_coding 1.729635 2.418718 1.613077 0.0522236 0.1602556 -0.581464 0.1336438 0.03589502 0.3590334 0.03589502 0.1459436 3.007342 0.0913375 0.01423333 0.2465000 0.2322667 0.03925679 0.03925679 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000172458 E001 0.0000000       13 20701995 20702016 22 +      
ENSG00000172458 E002 0.0000000       13 20702017 20702126 110 +      
ENSG00000172458 E003 0.0000000       13 20702127 20702134 8 +      
ENSG00000172458 E004 2.5423069 0.0066834577 4.338633e-03 1.409291e-02 13 20702135 20703320 1186 + 0.768 0.311 -2.218
ENSG00000172458 E005 0.0000000       13 20703321 20703342 22 +      
ENSG00000172458 E006 0.1515154 0.0448897125 3.277665e-01   13 20703343 20703444 102 + 0.148 0.000 -9.749
ENSG00000172458 E007 0.2924217 0.0290785164 1.103850e-01   13 20703445 20703627 183 + 0.258 0.000 -10.740
ENSG00000172458 E008 0.9244060 0.0139458449 2.912539e-02 7.024555e-02 13 20703699 20703995 297 + 0.481 0.101 -2.956
ENSG00000172458 E009 2.9433696 0.0057460556 6.663425e-05 3.638466e-04 13 20703996 20704078 83 + 0.874 0.251 -3.048
ENSG00000172458 E010 7.0375655 0.0030346039 3.105854e-02 7.402629e-02 13 20704079 20704291 213 + 1.030 0.776 -0.970
ENSG00000172458 E011 0.8576959 0.4087568664 6.033238e-01 7.241376e-01 13 20717098 20717369 272 + 0.347 0.187 -1.189
ENSG00000172458 E012 1.1448190 0.0125541395 3.558943e-01 5.002567e-01 13 20720546 20720769 224 + 0.419 0.251 -1.048
ENSG00000172458 E013 103.3304436 0.0005129716 1.622457e-09 2.313484e-08 13 20721636 20723300 1665 + 1.949 2.023 0.248