ENSG00000172171

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000541382 ENSG00000172171 HEK293_OSMI2_2hA HEK293_TMG_2hB TEFM protein_coding retained_intron 9.520956 4.015837 15.20562 0.5202624 0.2795654 1.918192 2.9653438 0.5474017 5.8511250 0.54740170 0.6543514 3.394388 0.24506667 0.1201000 0.38400000 0.26390000 0.147116017 0.003746737 FALSE  
ENST00000580840 ENSG00000172171 HEK293_OSMI2_2hA HEK293_TMG_2hB TEFM protein_coding protein_coding 9.520956 4.015837 15.20562 0.5202624 0.2795654 1.918192 0.7236761 0.4221408 0.5970926 0.02898522 0.0893378 0.490415 0.09959167 0.1074667 0.03913333 -0.06833333 0.003746737 0.003746737 FALSE  
ENST00000581216 ENSG00000172171 HEK293_OSMI2_2hA HEK293_TMG_2hB TEFM protein_coding protein_coding 9.520956 4.015837 15.20562 0.5202624 0.2795654 1.918192 5.4497569 3.0100704 7.8769832 0.49775957 0.2610074 1.384891 0.62227500 0.7644667 0.51893333 -0.24553333 0.173816828 0.003746737 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000172171 E001 1.564796 0.1038325645 0.6530008819 0.763598452 17 30897336 30897760 425 - 0.416 0.314 -0.599
ENSG00000172171 E002 1.186752 0.1658117294 0.0868165172 0.170300680 17 30898908 30898985 78 - 0.415 0.001 -10.238
ENSG00000172171 E003 90.629352 0.0007252440 0.0287568579 0.069516013 17 30898986 30899606 621 - 1.909 1.983 0.249
ENSG00000172171 E004 13.633224 0.0013508828 0.0006012896 0.002537931 17 30899607 30900412 806 - 1.017 1.324 1.095
ENSG00000172171 E005 51.923558 0.0004946941 0.7520152809 0.838347156 17 30900413 30900521 109 - 1.699 1.682 -0.059
ENSG00000172171 E006 32.185025 0.0007243791 0.5228187102 0.656562790 17 30900522 30900562 41 - 1.506 1.463 -0.147
ENSG00000172171 E007 1.401627 0.2211629353 0.3959806426 0.539766909 17 30904039 30904065 27 - 0.415 0.187 -1.574
ENSG00000172171 E008 122.687780 0.0002831646 0.1482047021 0.259239520 17 30904066 30904529 464 - 2.076 2.033 -0.145
ENSG00000172171 E009 34.379413 0.0032639663 0.1196765112 0.219508322 17 30906168 30906238 71 - 1.552 1.446 -0.363