ENSG00000171763

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000305560 ENSG00000171763 HEK293_OSMI2_2hA HEK293_TMG_2hB SPATA5L1 protein_coding protein_coding 9.291134 7.924797 11.4841 0.3511987 0.7530539 0.5346288 4.3220736 3.5183453 5.1441150 0.19800000 0.53800381 0.5467330 0.48482917 0.44606667 0.45733333 0.01126667 9.887352e-01 5.82399e-11 FALSE TRUE
ENST00000531624 ENSG00000171763 HEK293_OSMI2_2hA HEK293_TMG_2hB SPATA5L1 protein_coding protein_coding 9.291134 7.924797 11.4841 0.3511987 0.7530539 0.5346288 0.4800370 1.7419207 0.0000000 0.88390098 0.00000000 -7.4527937 0.05221667 0.21940000 0.00000000 -0.21940000 1.492956e-01 5.82399e-11 FALSE TRUE
ENST00000531970 ENSG00000171763 HEK293_OSMI2_2hA HEK293_TMG_2hB SPATA5L1 protein_coding nonsense_mediated_decay 9.291134 7.924797 11.4841 0.3511987 0.7530539 0.5346288 1.0428840 0.6084098 1.1414355 0.24038750 0.33430256 0.8967985 0.10990417 0.07450000 0.09756667 0.02306667 7.992617e-01 5.82399e-11 TRUE TRUE
ENST00000533199 ENSG00000171763 HEK293_OSMI2_2hA HEK293_TMG_2hB SPATA5L1 protein_coding retained_intron 9.291134 7.924797 11.4841 0.3511987 0.7530539 0.5346288 0.4900538 0.2887111 0.6029350 0.06911630 0.03897609 1.0369832 0.05262083 0.03583333 0.05306667 0.01723333 5.127716e-01 5.82399e-11   FALSE
ENST00000559860 ENSG00000171763 HEK293_OSMI2_2hA HEK293_TMG_2hB SPATA5L1 protein_coding retained_intron 9.291134 7.924797 11.4841 0.3511987 0.7530539 0.5346288 0.2902180 0.0000000 0.8091928 0.00000000 0.04552697 6.3561311 0.02859167 0.00000000 0.07103333 0.07103333 5.823990e-11 5.82399e-11 FALSE TRUE
MSTRG.10721.2 ENSG00000171763 HEK293_OSMI2_2hA HEK293_TMG_2hB SPATA5L1 protein_coding   9.291134 7.924797 11.4841 0.3511987 0.7530539 0.5346288 0.2717359 0.1100463 1.0195459 0.07679657 0.14713372 3.1003457 0.02716250 0.01466667 0.08833333 0.07366667 1.195431e-02 5.82399e-11 FALSE TRUE
MSTRG.10721.5 ENSG00000171763 HEK293_OSMI2_2hA HEK293_TMG_2hB SPATA5L1 protein_coding   9.291134 7.924797 11.4841 0.3511987 0.7530539 0.5346288 0.5581124 0.5319154 0.3455342 0.07762057 0.07086301 -0.6080793 0.06061250 0.06673333 0.02973333 -0.03700000 5.097903e-02 5.82399e-11 FALSE TRUE
MSTRG.10721.6 ENSG00000171763 HEK293_OSMI2_2hA HEK293_TMG_2hB SPATA5L1 protein_coding   9.291134 7.924797 11.4841 0.3511987 0.7530539 0.5346288 1.7001167 1.1254480 2.4213454 0.79106131 0.90731446 1.0984933 0.17065833 0.14283333 0.20290000 0.06006667 7.172651e-01 5.82399e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000171763 E001 4.8048368 0.0438560029 2.799437e-01 4.197428e-01 15 45402336 45402369 34 + 0.827 0.643 -0.750
ENSG00000171763 E002 5.3207920 0.0063621505 5.561173e-01 6.848437e-01 15 45402370 45402374 5 + 0.826 0.738 -0.354
ENSG00000171763 E003 98.3100987 0.0107934354 3.312166e-02 7.804961e-02 15 45402375 45403249 875 + 2.057 1.920 -0.457
ENSG00000171763 E004 60.9887466 0.0060832607 9.066050e-02 1.761651e-01 15 45403250 45403518 269 + 1.842 1.737 -0.353
ENSG00000171763 E005 69.3030573 0.0004451883 9.146598e-01 9.499827e-01 15 45405320 45405458 139 + 1.856 1.841 -0.051
ENSG00000171763 E006 48.0029614 0.0008401328 7.618298e-01 8.455962e-01 15 45405459 45405505 47 + 1.700 1.673 -0.092
ENSG00000171763 E007 1.6357727 0.0241636852 1.631079e-02 4.341327e-02 15 45407029 45407061 33 + 0.586 0.137 -2.945
ENSG00000171763 E008 5.7280322 0.0028807338 4.992518e-01 6.355703e-01 15 45407062 45407223 162 + 0.788 0.864 0.297
ENSG00000171763 E009 37.2990411 0.0006522748 7.471699e-01 8.347384e-01 15 45410372 45410372 1 + 1.574 1.581 0.024
ENSG00000171763 E010 96.0998219 0.0004179175 2.668466e-01 4.051036e-01 15 45410373 45410530 158 + 2.010 1.960 -0.167
ENSG00000171763 E011 101.0980929 0.0069480136 7.915940e-02 1.581677e-01 15 45414571 45414780 210 + 2.053 1.959 -0.315
ENSG00000171763 E012 118.9635573 0.0003189426 1.243238e-01 2.261624e-01 15 45415587 45415779 193 + 2.105 2.046 -0.197
ENSG00000171763 E013 7.8593145 0.0078366370 2.557977e-02 6.307691e-02 15 45415780 45415809 30 + 1.052 0.766 -1.087
ENSG00000171763 E014 1.8102039 0.0077043786 8.466376e-03 2.495223e-02 15 45415810 45415905 96 + 0.617 0.137 -3.079
ENSG00000171763 E015 6.9782354 0.0025947803 7.312757e-02 1.485200e-01 15 45416854 45417268 415 + 0.993 0.765 -0.874
ENSG00000171763 E016 64.5730591 0.0003794388 6.726465e-02 1.388090e-01 15 45417269 45417310 42 + 1.783 1.850 0.227
ENSG00000171763 E017 82.9876793 0.0038074185 7.598430e-02 1.530856e-01 15 45417311 45417399 89 + 1.889 1.959 0.234
ENSG00000171763 E018 0.5911862 0.0172671820 7.000358e-02 1.433758e-01 15 45417695 45417794 100 + 0.331 0.000 -10.377
ENSG00000171763 E019 84.9178143 0.0008194846 1.292239e-05 8.363596e-05 15 45418557 45418682 126 + 1.852 2.008 0.524
ENSG00000171763 E020 85.5877420 0.0003508646 5.073704e-08 5.461973e-07 15 45421043 45421415 373 + 1.835 2.025 0.642