ENSG00000171497

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000307720 ENSG00000171497 HEK293_OSMI2_2hA HEK293_TMG_2hB PPID protein_coding protein_coding 33.515 21.12241 41.58209 0.5807465 0.2733606 0.9768519 29.836013 20.4766426 37.37683 0.6722724 0.6632707 0.8678466 0.89557917 0.969133333 0.8991333 -0.07000000 0.004603443 0.004603443 FALSE  
ENST00000512699 ENSG00000171497 HEK293_OSMI2_2hA HEK293_TMG_2hB PPID protein_coding nonsense_mediated_decay 33.515 21.12241 41.58209 0.5807465 0.2733606 0.9768519 2.548868 0.1426262 2.97958 0.1426262 0.6496816 4.2918683 0.07132917 0.007133333 0.0715000 0.06436667 0.042826691 0.004603443 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000171497 E001 349.14421 0.0050758632 4.126296e-05 2.370987e-04 4 158709127 158709760 634 - 2.462 2.628 0.553
ENSG00000171497 E002 227.06219 0.0003679214 9.130120e-02 1.771572e-01 4 158709761 158709824 64 - 2.329 2.385 0.185
ENSG00000171497 E003 21.61218 0.0073236872 3.201378e-01 4.632537e-01 4 158709942 158710627 686 - 1.378 1.309 -0.240
ENSG00000171497 E004 221.95618 0.0008352240 5.886568e-01 7.118509e-01 4 158710628 158710670 43 - 2.332 2.362 0.097
ENSG00000171497 E005 269.86564 0.0005661254 9.949750e-01 1.000000e+00 4 158710762 158710848 87 - 2.423 2.437 0.048
ENSG00000171497 E006 280.67070 0.0002283584 8.819454e-01 9.283586e-01 4 158713119 158713260 142 - 2.436 2.453 0.057
ENSG00000171497 E007 178.24445 0.0029261585 3.744903e-02 8.630908e-02 4 158715297 158715403 107 - 2.206 2.294 0.293
ENSG00000171497 E008 213.61700 0.0021873624 3.750378e-01 5.194490e-01 4 158715562 158715668 107 - 2.306 2.347 0.137
ENSG00000171497 E009 138.61357 0.0003387758 5.721800e-01 6.983280e-01 4 158715669 158715684 16 - 2.139 2.135 -0.013
ENSG00000171497 E010 322.70199 0.0014508175 2.642513e-01 4.022125e-01 4 158717012 158717200 189 - 2.509 2.494 -0.051
ENSG00000171497 E011 213.40958 0.0024952043 1.438343e-01 2.532291e-01 4 158719180 158719286 107 - 2.340 2.304 -0.121
ENSG00000171497 E012 260.71843 0.0001747221 9.502890e-09 1.177047e-07 4 158721343 158721483 141 - 2.457 2.343 -0.381
ENSG00000171497 E013 196.84312 0.0006868210 1.030402e-08 1.266917e-07 4 158723204 158723396 193 - 2.346 2.199 -0.491