ENSG00000171365

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000376088 ENSG00000171365 HEK293_OSMI2_2hA HEK293_TMG_2hB CLCN5 protein_coding protein_coding 3.334838 1.72671 3.539328 0.1621244 0.1017085 1.031189 0.36865668 0.1985953 0.3656420 0.07688363 0.04981414 0.8486518 0.1024625 0.1122000 0.1034333 -0.008766667 0.941202713 0.000522638 FALSE TRUE
ENST00000376091 ENSG00000171365 HEK293_OSMI2_2hA HEK293_TMG_2hB CLCN5 protein_coding protein_coding 3.334838 1.72671 3.539328 0.1621244 0.1017085 1.031189 1.59261288 0.8658179 2.0847907 0.29864689 0.26835310 1.2581033 0.4741125 0.4920333 0.5880667 0.096033333 0.837406612 0.000522638 FALSE TRUE
ENST00000376108 ENSG00000171365 HEK293_OSMI2_2hA HEK293_TMG_2hB CLCN5 protein_coding protein_coding 3.334838 1.72671 3.539328 0.1621244 0.1017085 1.031189 0.46598673 0.0000000 0.5964856 0.00000000 0.18045410 5.9224014 0.1292792 0.0000000 0.1668667 0.166866667 0.000522638 0.000522638 FALSE TRUE
ENST00000642383 ENSG00000171365 HEK293_OSMI2_2hA HEK293_TMG_2hB CLCN5 protein_coding protein_coding 3.334838 1.72671 3.539328 0.1621244 0.1017085 1.031189 0.31233976 0.5043071 0.0000000 0.28906116 0.00000000 -5.6845582 0.1200917 0.2858333 0.0000000 -0.285833333 0.095168191 0.000522638 FALSE TRUE
ENST00000642885 ENSG00000171365 HEK293_OSMI2_2hA HEK293_TMG_2hB CLCN5 protein_coding protein_coding 3.334838 1.72671 3.539328 0.1621244 0.1017085 1.031189 0.08503512 0.1579902 0.0000000 0.15799021 0.00000000 -4.0703052 0.0363500 0.1099667 0.0000000 -0.109966667 0.702853071 0.000522638 FALSE TRUE
MSTRG.34249.3 ENSG00000171365 HEK293_OSMI2_2hA HEK293_TMG_2hB CLCN5 protein_coding   3.334838 1.72671 3.539328 0.1621244 0.1017085 1.031189 0.31552062 0.0000000 0.3851610 0.00000000 0.21170401 5.3043688 0.0806625 0.0000000 0.1120333 0.112033333 0.316607173 0.000522638 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000171365 E001 1.2295131 0.1353096745 2.467074e-01 3.820834e-01 X 49922596 49922614 19 + 0.372 0.190 -1.302
ENSG00000171365 E002 2.6289876 0.0258547310 8.850490e-01 9.305171e-01 X 49922615 49922654 40 + 0.495 0.631 0.627
ENSG00000171365 E003 8.8635064 0.0019765522 3.437852e-01 4.878473e-01 X 49922655 49922792 138 + 0.959 0.990 0.113
ENSG00000171365 E004 0.0000000       X 49923404 49923406 3 +      
ENSG00000171365 E005 10.9139954 0.0016445537 1.149303e-02 3.233372e-02 X 49923407 49923482 76 + 1.079 0.939 -0.519
ENSG00000171365 E006 3.9095161 0.0041176729 8.847892e-01 9.303421e-01 X 49923483 49923741 259 + 0.632 0.731 0.419
ENSG00000171365 E007 16.8570240 0.0010756419 4.113157e-03 1.346045e-02 X 49925171 49925314 144 + 1.250 1.134 -0.413
ENSG00000171365 E008 10.5409090 0.0024162598 1.294611e-03 4.958158e-03 X 50042316 50042337 22 + 1.086 0.847 -0.891
ENSG00000171365 E009 15.7558083 0.0139548449 2.118151e-03 7.590747e-03 X 50042338 50042462 125 + 1.240 1.037 -0.730
ENSG00000171365 E010 0.4439371 0.0215670916 2.608020e-01 3.983893e-01 X 50042463 50042541 79 + 0.198 0.000 -15.282
ENSG00000171365 E011 0.7458221 0.0193294028 5.182159e-01 6.524780e-01 X 50050283 50050413 131 + 0.249 0.190 -0.495
ENSG00000171365 E012 2.6466710 0.0122485894 3.268518e-04 1.488094e-03 X 50054440 50054633 194 + 0.651 0.000 -17.592
ENSG00000171365 E013 2.3627821 0.0061298098 6.489247e-04 2.712221e-03 X 50067576 50067760 185 + 0.612 0.000 -17.464
ENSG00000171365 E014 0.0000000       X 50067964 50068015 52 +      
ENSG00000171365 E015 0.1451727 0.0437928003 1.000000e+00   X 50068112 50068164 53 + 0.077 0.000 -13.690
ENSG00000171365 E016 0.2987644 0.0271856860 4.757498e-01   X 50069501 50069554 54 + 0.142 0.000 -14.711
ENSG00000171365 E017 0.2987644 0.0271856860 4.757498e-01   X 50069555 50069744 190 + 0.142 0.000 -14.711
ENSG00000171365 E018 0.0000000       X 50069745 50069878 134 +      
ENSG00000171365 E019 17.6344084 0.0014240294 3.423600e-03 1.150779e-02 X 50069879 50070030 152 + 1.269 1.151 -0.416
ENSG00000171365 E020 17.3772467 0.0012567866 4.836168e-03 1.547526e-02 X 50072489 50072588 100 + 1.260 1.151 -0.384
ENSG00000171365 E021 24.7437959 0.0009234065 2.205104e-02 5.578634e-02 X 50075795 50075982 188 + 1.383 1.361 -0.078
ENSG00000171365 E022 14.7885610 0.0183941756 1.769813e-01 2.973175e-01 X 50080594 50080716 123 + 1.167 1.152 -0.054
ENSG00000171365 E023 7.9076869 0.0275136347 7.229371e-01 8.169076e-01 X 50081641 50081664 24 + 0.889 0.966 0.291
ENSG00000171365 E024 17.4875910 0.0145030933 4.914039e-01 6.286720e-01 X 50081665 50081847 183 + 1.210 1.281 0.247
ENSG00000171365 E025 0.0000000       X 50085452 50085634 183 +      
ENSG00000171365 E026 9.9267173 0.0017381315 7.134945e-02 1.455735e-01 X 50085980 50086060 81 + 1.027 0.965 -0.231
ENSG00000171365 E027 35.3561092 0.0050621705 1.441087e-04 7.213890e-04 X 50086328 50086870 543 + 1.558 1.428 -0.446
ENSG00000171365 E028 0.1482932 0.0408747238 2.445191e-01   X 50088413 50088484 72 + 0.000 0.190 15.157
ENSG00000171365 E029 23.8396030 0.0116347217 3.117491e-02 7.423974e-02 X 50088698 50088884 187 + 1.376 1.326 -0.174
ENSG00000171365 E030 47.7572979 0.0130331826 1.896635e-03 6.903603e-03 X 50090116 50090514 399 + 1.678 1.582 -0.326
ENSG00000171365 E031 32.3820703 0.0053439622 2.943630e-01 4.354624e-01 X 50090670 50090886 217 + 1.462 1.531 0.237
ENSG00000171365 E032 495.8969606 0.0157200924 2.587493e-07 2.425857e-06 X 50092129 50099235 7107 + 2.557 2.832 0.916