ENSG00000171174

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000302188 ENSG00000171174 HEK293_OSMI2_2hA HEK293_TMG_2hB RBKS protein_coding protein_coding 2.424172 3.091489 3.386539 0.4472587 0.2253132 0.1311041 2.1734367 2.96025288 3.0045346 0.4657193 0.28847971 0.02134954 0.8948292 0.95333333 0.88363333 -0.06970000 0.14809759 0.02658403 FALSE  
ENST00000449378 ENSG00000171174 HEK293_OSMI2_2hA HEK293_TMG_2hB RBKS protein_coding nonsense_mediated_decay 2.424172 3.091489 3.386539 0.4472587 0.2253132 0.1311041 0.1378153 0.05771504 0.2666284 0.0170419 0.01120973 2.03040124 0.0604750 0.01783333 0.07906667 0.06123333 0.02658403 0.02658403 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000171174 E001 4.7544247 0.0033746058 0.2050054038 0.332411746 2 27781379 27781572 194 - 0.849 0.679 -0.682
ENSG00000171174 E002 30.7595530 0.0008021709 0.2727448035 0.411697267 2 27781573 27781788 216 - 1.471 1.536 0.221
ENSG00000171174 E003 0.8157519 0.0202549314 0.6076684919 0.727591440 2 27809687 27810045 359 - 0.303 0.212 -0.681
ENSG00000171174 E004 41.1231915 0.0005810084 0.0548652867 0.117763749 2 27827567 27827755 189 - 1.572 1.668 0.328
ENSG00000171174 E005 28.8756173 0.0007089312 0.1775882130 0.298107819 2 27832686 27832777 92 - 1.430 1.511 0.280
ENSG00000171174 E006 41.4323484 0.0012576830 0.5854011495 0.709099749 2 27843067 27843231 165 - 1.647 1.618 -0.098
ENSG00000171174 E007 24.5803556 0.0008772615 0.0980834099 0.187602813 2 27847042 27847104 63 - 1.466 1.356 -0.380
ENSG00000171174 E008 23.7402130 0.0009330839 0.4105843693 0.554186660 2 27848034 27848097 64 - 1.424 1.368 -0.195
ENSG00000171174 E009 22.8757310 0.0025930728 0.8172247833 0.884412233 2 27858439 27858500 62 - 1.366 1.380 0.050
ENSG00000171174 E010 25.3647548 0.0025430795 0.7480023231 0.835377736 2 27858501 27858571 71 - 1.408 1.428 0.071
ENSG00000171174 E011 0.0000000       2 27861413 27861546 134 -      
ENSG00000171174 E012 4.8799951 0.0034773735 0.0001333705 0.000673885 2 27869772 27870890 1119 - 0.989 0.461 -2.212
ENSG00000171174 E013 23.2264080 0.0009048810 0.9879745762 0.996526357 2 27890257 27890681 425 - 1.390 1.391 0.002