ENSG00000171016

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000302000 ENSG00000171016 HEK293_OSMI2_2hA HEK293_TMG_2hB PYGO1 protein_coding protein_coding 1.573759 0.3368383 2.952435 0.02830103 0.04922434 3.094448 0.70204913 0.2068826 1.3800824 0.1110087 0.01736529 2.680184 0.4642750 0.6160667 0.4678333 -0.1482333 0.743322081 0.002332486 FALSE TRUE
ENST00000563719 ENSG00000171016 HEK293_OSMI2_2hA HEK293_TMG_2hB PYGO1 protein_coding protein_coding 1.573759 0.3368383 2.952435 0.02830103 0.04922434 3.094448 0.05853653 0.1299557 0.0000000 0.1091415 0.00000000 -3.806898 0.1160583 0.3839333 0.0000000 -0.3839333 0.348822528 0.002332486 FALSE TRUE
ENST00000645724 ENSG00000171016 HEK293_OSMI2_2hA HEK293_TMG_2hB PYGO1 protein_coding protein_coding 1.573759 0.3368383 2.952435 0.02830103 0.04922434 3.094448 0.41527432 0.0000000 1.0520983 0.0000000 0.21881579 6.730773 0.2272000 0.0000000 0.3584333 0.3584333 0.002332486 0.002332486 FALSE TRUE
MSTRG.10841.3 ENSG00000171016 HEK293_OSMI2_2hA HEK293_TMG_2hB PYGO1 protein_coding   1.573759 0.3368383 2.952435 0.02830103 0.04922434 3.094448 0.39789941 0.0000000 0.5202543 0.0000000 0.26111742 5.728612 0.1924583 0.0000000 0.1737333 0.1737333 0.573424185 0.002332486 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000171016 E001 0.0000000       15 55538884 55538889 6 -      
ENSG00000171016 E002 5.1669253 0.0034552938 0.0006416767 0.002686313 15 55538890 55539094 205 - 0.579 1.044 1.850
ENSG00000171016 E003 4.9862650 0.0035229066 0.0004349264 0.001908850 15 55539095 55539134 40 - 0.562 1.044 1.928
ENSG00000171016 E004 95.0619025 0.0004604712 0.0002675861 0.001247622 15 55539135 55543583 4449 - 1.857 1.986 0.435
ENSG00000171016 E005 25.1693190 0.0008892649 0.6919235429 0.793278223 15 55543584 55544408 825 - 1.328 1.295 -0.116
ENSG00000171016 E006 44.1262592 0.0005578798 0.6792342343 0.783491583 15 55544409 55545225 817 - 1.551 1.577 0.089
ENSG00000171016 E007 24.0695816 0.0009775887 0.1143080810 0.211823218 15 55545226 55546004 779 - 1.331 1.188 -0.505
ENSG00000171016 E008 58.4841884 0.0005916963 0.0003537047 0.001594487 15 55546005 55547147 1143 - 1.714 1.505 -0.712
ENSG00000171016 E009 10.1916503 0.0017030132 0.0013002181 0.004977216 15 55548910 55548995 86 - 1.024 0.500 -2.145
ENSG00000171016 E010 10.3106850 0.0016154925 0.0048384252 0.015481441 15 55587835 55588345 511 - 1.024 0.589 -1.730
ENSG00000171016 E011 0.9117205 0.0583076974 1.0000000000 1.000000000 15 55588804 55588947 144 - 0.239 0.236 -0.026