ENSG00000170525

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000379775 ENSG00000170525 HEK293_OSMI2_2hA HEK293_TMG_2hB PFKFB3 protein_coding protein_coding 23.67595 29.83801 19.08164 1.409194 0.08851811 -0.6446935 1.139504 1.354449 2.1530023 0.3151726 0.3810356 0.66471648 0.04936667 0.04543333 0.11296667 0.06753333 0.03484622 0.03484622 FALSE TRUE
ENST00000379789 ENSG00000170525 HEK293_OSMI2_2hA HEK293_TMG_2hB PFKFB3 protein_coding protein_coding 23.67595 29.83801 19.08164 1.409194 0.08851811 -0.6446935 13.681380 14.487893 11.9056249 1.1751435 0.7182223 -0.28298868 0.59269583 0.48546667 0.62363333 0.13816667 0.10304989 0.03484622 FALSE TRUE
ENST00000441697 ENSG00000170525 HEK293_OSMI2_2hA HEK293_TMG_2hB PFKFB3 protein_coding protein_coding 23.67595 29.83801 19.08164 1.409194 0.08851811 -0.6446935 3.671489 8.867953 0.5690926 1.2810906 0.2848294 -3.93836124 0.13487083 0.29770000 0.02993333 -0.26776667 0.05105490 0.03484622 FALSE TRUE
MSTRG.3496.1 ENSG00000170525 HEK293_OSMI2_2hA HEK293_TMG_2hB PFKFB3 protein_coding   23.67595 29.83801 19.08164 1.409194 0.08851811 -0.6446935 2.644705 3.000987 2.8865449 0.4227067 0.2362764 -0.05590353 0.11336250 0.10003333 0.15136667 0.05133333 0.13981572 0.03484622 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000170525 E001 13.6752760 0.0013312185 1.815162e-01 3.031430e-01 10 6144918 6144933 16 + 1.201 1.112 -0.317
ENSG00000170525 E002 60.7702914 0.0007830757 2.921752e-05 1.743142e-04 10 6144934 6145013 80 + 1.864 1.705 -0.535
ENSG00000170525 E003 0.0000000       10 6146316 6146496 181 +      
ENSG00000170525 E004 5.7422963 0.0030050661 2.463056e-01 3.816233e-01 10 6163833 6164011 179 + 0.683 0.869 0.745
ENSG00000170525 E005 0.0000000       10 6202866 6202876 11 +      
ENSG00000170525 E006 0.0000000       10 6202877 6202899 23 +      
ENSG00000170525 E007 0.0000000       10 6202900 6202931 32 +      
ENSG00000170525 E008 26.9585975 0.0024288454 9.564742e-04 3.809745e-03 10 6202932 6203336 405 + 1.543 1.350 -0.667
ENSG00000170525 E009 129.1756980 0.0005148102 8.937765e-08 9.156069e-07 10 6213623 6213748 126 + 2.179 2.042 -0.457
ENSG00000170525 E010 126.9808926 0.0004886486 5.617973e-04 2.391328e-03 10 6215221 6215317 97 + 2.140 2.063 -0.256
ENSG00000170525 E011 131.3936320 0.0004416394 2.409505e-01 3.752906e-01 10 6216125 6216191 67 + 2.110 2.108 -0.007
ENSG00000170525 E012 138.0380950 0.0006152604 1.649764e-01 2.816672e-01 10 6216706 6216780 75 + 2.135 2.126 -0.029
ENSG00000170525 E013 114.9771516 0.0002654590 1.598280e-03 5.955731e-03 10 6217135 6217191 57 + 2.091 2.022 -0.234
ENSG00000170525 E014 179.6339860 0.0007842965 1.027314e-02 2.943860e-02 10 6219569 6219693 125 + 2.265 2.226 -0.129
ENSG00000170525 E015 192.2102741 0.0010381371 1.245166e-03 4.793987e-03 10 6220658 6220865 208 + 2.307 2.249 -0.193
ENSG00000170525 E016 74.9902486 0.0003541521 2.920293e-01 4.329589e-01 10 6221381 6221403 23 + 1.873 1.864 -0.028
ENSG00000170525 E017 142.5400461 0.0002571962 4.188319e-01 5.620829e-01 10 6221404 6221527 124 + 2.135 2.146 0.036
ENSG00000170525 E018 120.6248376 0.0003064946 6.924757e-01 7.937189e-01 10 6221641 6221745 105 + 2.054 2.076 0.074
ENSG00000170525 E019 1.1501176 0.0126131638 9.904093e-02 1.890022e-01 10 6222708 6222854 147 + 0.464 0.192 -1.779
ENSG00000170525 E020 138.1289717 0.0020863005 3.964079e-01 5.401744e-01 10 6222855 6222984 130 + 2.125 2.129 0.013
ENSG00000170525 E021 119.3222248 0.0005265212 9.811396e-03 2.829735e-02 10 6223958 6224020 63 + 2.097 2.045 -0.173
ENSG00000170525 E022 88.2607882 0.0005677820 3.971248e-03 1.306102e-02 10 6224149 6224165 17 + 1.984 1.908 -0.255
ENSG00000170525 E023 106.5147290 0.0021051211 2.192582e-02 5.551996e-02 10 6224166 6224213 48 + 2.052 1.996 -0.190
ENSG00000170525 E024 0.5911836 0.0390226773 1.665511e-01 2.837081e-01 10 6224506 6224623 118 + 0.000 0.264 9.869
ENSG00000170525 E025 0.8836184 0.0170725960 9.934308e-01 1.000000e+00 10 6225161 6225248 88 + 0.246 0.263 0.129
ENSG00000170525 E026 165.2206474 0.0005039432 9.954970e-01 1.000000e+00 10 6226192 6226365 174 + 2.180 2.216 0.119
ENSG00000170525 E027 2.0723178 0.0708865122 5.434925e-01 6.740967e-01 10 6226366 6226447 82 + 0.517 0.429 -0.443
ENSG00000170525 E028 2.5088045 0.0058492438 9.912761e-01 9.986442e-01 10 6228196 6228218 23 + 0.517 0.544 0.127
ENSG00000170525 E029 1.8403016 0.0082818050 8.005164e-01 8.728571e-01 10 6228219 6228295 77 + 0.402 0.469 0.349
ENSG00000170525 E030 1.3212103 0.0123040775 1.385226e-01 2.460452e-01 10 6229109 6229180 72 + 0.140 0.426 2.130
ENSG00000170525 E031 2.5162800 0.0063452181 6.146981e-01 7.331032e-01 10 6231263 6231314 52 + 0.565 0.508 -0.265
ENSG00000170525 E032 1710.7662909 0.0012556470 1.998307e-15 7.257923e-14 10 6232895 6235532 2638 + 3.130 3.264 0.445
ENSG00000170525 E033 4.6371571 0.0035956345 5.137571e-02 1.115988e-01 10 6254178 6254644 467 + 0.517 0.834 1.349